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Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil
In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569210/ https://www.ncbi.nlm.nih.gov/pubmed/34737284 http://dx.doi.org/10.1038/s41467-021-26462-x |
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author | Pogodalla, Nicole Kranenburg, Holger Rey, Simone Rodrigues, Silke Cardona, Albert Klämbt, Christian |
author_facet | Pogodalla, Nicole Kranenburg, Holger Rey, Simone Rodrigues, Silke Cardona, Albert Klämbt, Christian |
author_sort | Pogodalla, Nicole |
collection | PubMed |
description | In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP(3)) and the Na(+)/K(+)-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP(2)) that is supported by a sub-membranous ß(Heavy)-Spectrin cytoskeleton. ß(Heavy)-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP(2) and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function. |
format | Online Article Text |
id | pubmed-8569210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85692102021-11-15 Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil Pogodalla, Nicole Kranenburg, Holger Rey, Simone Rodrigues, Silke Cardona, Albert Klämbt, Christian Nat Commun Article In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP(3)) and the Na(+)/K(+)-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP(2)) that is supported by a sub-membranous ß(Heavy)-Spectrin cytoskeleton. ß(Heavy)-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP(2) and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function. Nature Publishing Group UK 2021-11-04 /pmc/articles/PMC8569210/ /pubmed/34737284 http://dx.doi.org/10.1038/s41467-021-26462-x Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pogodalla, Nicole Kranenburg, Holger Rey, Simone Rodrigues, Silke Cardona, Albert Klämbt, Christian Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title | Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title_full | Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title_fullStr | Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title_full_unstemmed | Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title_short | Drosophila ß(Heavy)-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
title_sort | drosophila ß(heavy)-spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569210/ https://www.ncbi.nlm.nih.gov/pubmed/34737284 http://dx.doi.org/10.1038/s41467-021-26462-x |
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