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Biological evaluation of linalool on the function of blood vessels
Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569525/ https://www.ncbi.nlm.nih.gov/pubmed/34713293 http://dx.doi.org/10.3892/mmr.2021.12514 |
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author | Liang, Yunyu Zhong, Yan Li, Xinmei Xiao, Yingying Wu, Yu Xie, Pingchang |
author_facet | Liang, Yunyu Zhong, Yan Li, Xinmei Xiao, Yingying Wu, Yu Xie, Pingchang |
author_sort | Liang, Yunyu |
collection | PubMed |
description | Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explore the role and underlying mechanism of linalool in the physiological behavior of VSMCs. Angiotensin II (Ang II) was utilized to treat VSMCs, and MTT and western blotting assays were then employed to detect the effect of linalool on the induced proliferation and migration of VSMCs. The target gene of linalool was predicted by the SwissTargetPrediction website, and its expression level in VSMCs was determined using reverse transcription-quantitative PCR and western blotting. Next, the role of the target gene in the physiological behavior of VSMCs treated with linalool was examined, and the signaling pathway was explored. The results revealed that the proliferation and migration of VSMCs treated with Ang II were significantly promoted, and linalool could alleviate these effects in a dose-dependent manner. Cholinergic receptor muscarinic 3 (CHRM3), as a predicted target, was found to be highly expressed in Ang II-induced VSMCs, and CHRM3 overexpression could prevent the inhibitory effect of linalool on cell proliferation and migration. In addition, its overexpression caused an increase in the expression of proteins related to the MAPK signaling pathway. In conclusion, linalool inhibited the proliferation and migration of Ang II-induced VSMCs and blocked the MAPK signaling pathway by downregulating CHRM3. |
format | Online Article Text |
id | pubmed-8569525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-85695252021-12-07 Biological evaluation of linalool on the function of blood vessels Liang, Yunyu Zhong, Yan Li, Xinmei Xiao, Yingying Wu, Yu Xie, Pingchang Mol Med Rep Articles Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explore the role and underlying mechanism of linalool in the physiological behavior of VSMCs. Angiotensin II (Ang II) was utilized to treat VSMCs, and MTT and western blotting assays were then employed to detect the effect of linalool on the induced proliferation and migration of VSMCs. The target gene of linalool was predicted by the SwissTargetPrediction website, and its expression level in VSMCs was determined using reverse transcription-quantitative PCR and western blotting. Next, the role of the target gene in the physiological behavior of VSMCs treated with linalool was examined, and the signaling pathway was explored. The results revealed that the proliferation and migration of VSMCs treated with Ang II were significantly promoted, and linalool could alleviate these effects in a dose-dependent manner. Cholinergic receptor muscarinic 3 (CHRM3), as a predicted target, was found to be highly expressed in Ang II-induced VSMCs, and CHRM3 overexpression could prevent the inhibitory effect of linalool on cell proliferation and migration. In addition, its overexpression caused an increase in the expression of proteins related to the MAPK signaling pathway. In conclusion, linalool inhibited the proliferation and migration of Ang II-induced VSMCs and blocked the MAPK signaling pathway by downregulating CHRM3. D.A. Spandidos 2021-12 2021-10-28 /pmc/articles/PMC8569525/ /pubmed/34713293 http://dx.doi.org/10.3892/mmr.2021.12514 Text en Copyright: © Liang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liang, Yunyu Zhong, Yan Li, Xinmei Xiao, Yingying Wu, Yu Xie, Pingchang Biological evaluation of linalool on the function of blood vessels |
title | Biological evaluation of linalool on the function of blood vessels |
title_full | Biological evaluation of linalool on the function of blood vessels |
title_fullStr | Biological evaluation of linalool on the function of blood vessels |
title_full_unstemmed | Biological evaluation of linalool on the function of blood vessels |
title_short | Biological evaluation of linalool on the function of blood vessels |
title_sort | biological evaluation of linalool on the function of blood vessels |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569525/ https://www.ncbi.nlm.nih.gov/pubmed/34713293 http://dx.doi.org/10.3892/mmr.2021.12514 |
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