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Biological evaluation of linalool on the function of blood vessels

Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explo...

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Autores principales: Liang, Yunyu, Zhong, Yan, Li, Xinmei, Xiao, Yingying, Wu, Yu, Xie, Pingchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569525/
https://www.ncbi.nlm.nih.gov/pubmed/34713293
http://dx.doi.org/10.3892/mmr.2021.12514
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author Liang, Yunyu
Zhong, Yan
Li, Xinmei
Xiao, Yingying
Wu, Yu
Xie, Pingchang
author_facet Liang, Yunyu
Zhong, Yan
Li, Xinmei
Xiao, Yingying
Wu, Yu
Xie, Pingchang
author_sort Liang, Yunyu
collection PubMed
description Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explore the role and underlying mechanism of linalool in the physiological behavior of VSMCs. Angiotensin II (Ang II) was utilized to treat VSMCs, and MTT and western blotting assays were then employed to detect the effect of linalool on the induced proliferation and migration of VSMCs. The target gene of linalool was predicted by the SwissTargetPrediction website, and its expression level in VSMCs was determined using reverse transcription-quantitative PCR and western blotting. Next, the role of the target gene in the physiological behavior of VSMCs treated with linalool was examined, and the signaling pathway was explored. The results revealed that the proliferation and migration of VSMCs treated with Ang II were significantly promoted, and linalool could alleviate these effects in a dose-dependent manner. Cholinergic receptor muscarinic 3 (CHRM3), as a predicted target, was found to be highly expressed in Ang II-induced VSMCs, and CHRM3 overexpression could prevent the inhibitory effect of linalool on cell proliferation and migration. In addition, its overexpression caused an increase in the expression of proteins related to the MAPK signaling pathway. In conclusion, linalool inhibited the proliferation and migration of Ang II-induced VSMCs and blocked the MAPK signaling pathway by downregulating CHRM3.
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spelling pubmed-85695252021-12-07 Biological evaluation of linalool on the function of blood vessels Liang, Yunyu Zhong, Yan Li, Xinmei Xiao, Yingying Wu, Yu Xie, Pingchang Mol Med Rep Articles Long-term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explore the role and underlying mechanism of linalool in the physiological behavior of VSMCs. Angiotensin II (Ang II) was utilized to treat VSMCs, and MTT and western blotting assays were then employed to detect the effect of linalool on the induced proliferation and migration of VSMCs. The target gene of linalool was predicted by the SwissTargetPrediction website, and its expression level in VSMCs was determined using reverse transcription-quantitative PCR and western blotting. Next, the role of the target gene in the physiological behavior of VSMCs treated with linalool was examined, and the signaling pathway was explored. The results revealed that the proliferation and migration of VSMCs treated with Ang II were significantly promoted, and linalool could alleviate these effects in a dose-dependent manner. Cholinergic receptor muscarinic 3 (CHRM3), as a predicted target, was found to be highly expressed in Ang II-induced VSMCs, and CHRM3 overexpression could prevent the inhibitory effect of linalool on cell proliferation and migration. In addition, its overexpression caused an increase in the expression of proteins related to the MAPK signaling pathway. In conclusion, linalool inhibited the proliferation and migration of Ang II-induced VSMCs and blocked the MAPK signaling pathway by downregulating CHRM3. D.A. Spandidos 2021-12 2021-10-28 /pmc/articles/PMC8569525/ /pubmed/34713293 http://dx.doi.org/10.3892/mmr.2021.12514 Text en Copyright: © Liang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liang, Yunyu
Zhong, Yan
Li, Xinmei
Xiao, Yingying
Wu, Yu
Xie, Pingchang
Biological evaluation of linalool on the function of blood vessels
title Biological evaluation of linalool on the function of blood vessels
title_full Biological evaluation of linalool on the function of blood vessels
title_fullStr Biological evaluation of linalool on the function of blood vessels
title_full_unstemmed Biological evaluation of linalool on the function of blood vessels
title_short Biological evaluation of linalool on the function of blood vessels
title_sort biological evaluation of linalool on the function of blood vessels
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569525/
https://www.ncbi.nlm.nih.gov/pubmed/34713293
http://dx.doi.org/10.3892/mmr.2021.12514
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