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Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption

The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial i...

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Autores principales: Vélez, Jessica M Bedoya, Martínez, José Gregorio, Ospina, Juliana Tobón, Agudelo, Susana Ochoa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569635/
https://www.ncbi.nlm.nih.gov/pubmed/34765463
http://dx.doi.org/10.1016/j.btre.2021.e00685
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author Vélez, Jessica M Bedoya
Martínez, José Gregorio
Ospina, Juliana Tobón
Agudelo, Susana Ochoa
author_facet Vélez, Jessica M Bedoya
Martínez, José Gregorio
Ospina, Juliana Tobón
Agudelo, Susana Ochoa
author_sort Vélez, Jessica M Bedoya
collection PubMed
description The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial isolates were obtained from wastewater bodies from an industrial zone in the municipality of Girardota, Antioquia, Colombia. Subsequently, they were selected to evaluate their lead tolerance capacity at different concentrations. In addition, some parameters were determined, such as the capacity to produce exopolysaccharides and their biosorption to understand potential mechanisms associated to lead tolerance. According to the biocehemical test (Vitek) and the molecular analysis of sequences of 16S rDNA, bacterial were identified as Pseudomonas aeruginosa, Pseudomonas nitroreducens, and Pseudomonas alcaligenes. We determined that the seven isolates had the capacity to tolerate concentrations higher than 50 mg/ml of lead, and that the concentration and exposure time (40 h) to this metal significantly affect the Pseudomonas spp. isolates. Statistically significant differences were detected (p < 0.05) in the production of the exopolysaccharide (EPS) among the isolates. P. aeruginosa (P16) was the strain with the maximum absorbance exopolysaccharide measured. We evidenced that P. aeruginosa (P14) and P. nitroreducens (P20) have 80% capacity to biosorber lead using live mass (minimum range from 80.9% to 87%). It is suggested that the tolerance to lead exhibited by the environmental isolates of Pseudomonas spp. can be attributed to the production of exopolysaccharides and biosorption, which are protection factors for its survival in contaminated places. Finally, it was determined that the adsorption measured from dead biomass was significant (p < 0.05) from 40 h of exposure to metal (Average 182.2 ± 7). We generated new knowledge about the potential use of the Pseudomonas spp. genus to bioremediate affluent contaminated with heavy metals.
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spelling pubmed-85696352021-11-10 Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption Vélez, Jessica M Bedoya Martínez, José Gregorio Ospina, Juliana Tobón Agudelo, Susana Ochoa Biotechnol Rep (Amst) Short Communication The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial isolates were obtained from wastewater bodies from an industrial zone in the municipality of Girardota, Antioquia, Colombia. Subsequently, they were selected to evaluate their lead tolerance capacity at different concentrations. In addition, some parameters were determined, such as the capacity to produce exopolysaccharides and their biosorption to understand potential mechanisms associated to lead tolerance. According to the biocehemical test (Vitek) and the molecular analysis of sequences of 16S rDNA, bacterial were identified as Pseudomonas aeruginosa, Pseudomonas nitroreducens, and Pseudomonas alcaligenes. We determined that the seven isolates had the capacity to tolerate concentrations higher than 50 mg/ml of lead, and that the concentration and exposure time (40 h) to this metal significantly affect the Pseudomonas spp. isolates. Statistically significant differences were detected (p < 0.05) in the production of the exopolysaccharide (EPS) among the isolates. P. aeruginosa (P16) was the strain with the maximum absorbance exopolysaccharide measured. We evidenced that P. aeruginosa (P14) and P. nitroreducens (P20) have 80% capacity to biosorber lead using live mass (minimum range from 80.9% to 87%). It is suggested that the tolerance to lead exhibited by the environmental isolates of Pseudomonas spp. can be attributed to the production of exopolysaccharides and biosorption, which are protection factors for its survival in contaminated places. Finally, it was determined that the adsorption measured from dead biomass was significant (p < 0.05) from 40 h of exposure to metal (Average 182.2 ± 7). We generated new knowledge about the potential use of the Pseudomonas spp. genus to bioremediate affluent contaminated with heavy metals. Elsevier 2021-10-29 /pmc/articles/PMC8569635/ /pubmed/34765463 http://dx.doi.org/10.1016/j.btre.2021.e00685 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Vélez, Jessica M Bedoya
Martínez, José Gregorio
Ospina, Juliana Tobón
Agudelo, Susana Ochoa
Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title_full Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title_fullStr Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title_full_unstemmed Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title_short Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
title_sort bioremediation potential of pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569635/
https://www.ncbi.nlm.nih.gov/pubmed/34765463
http://dx.doi.org/10.1016/j.btre.2021.e00685
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