Cargando…

Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes

PURPOSE: There has been an interest in the microbial azo dye degradation as an optional method for the treatment of azo dye-containing wastes. Tattoo ink is an extremely unique azo dye-rich environment, which have never been explored in terms of microorganisms capable of degrading azo dyes. Previous...

Descripción completa

Detalles Bibliográficos
Autores principales: Nho, Seong Won, Cui, Xuewen, Kweon, Ohgew, Jin, Jinshan, Chen, Huizhong, Moon, Mi Sun, Kim, Seong-Jae, Cerniglia, Carl E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569640/
https://www.ncbi.nlm.nih.gov/pubmed/34744534
http://dx.doi.org/10.1186/s13213-021-01648-2
_version_ 1784594678350348288
author Nho, Seong Won
Cui, Xuewen
Kweon, Ohgew
Jin, Jinshan
Chen, Huizhong
Moon, Mi Sun
Kim, Seong-Jae
Cerniglia, Carl E.
author_facet Nho, Seong Won
Cui, Xuewen
Kweon, Ohgew
Jin, Jinshan
Chen, Huizhong
Moon, Mi Sun
Kim, Seong-Jae
Cerniglia, Carl E.
author_sort Nho, Seong Won
collection PubMed
description PURPOSE: There has been an interest in the microbial azo dye degradation as an optional method for the treatment of azo dye-containing wastes. Tattoo ink is an extremely unique azo dye-rich environment, which have never been explored in terms of microorganisms capable of degrading azo dyes. Previously, we isolated 81 phylogenetically diverse bacteria, belonging to 18 genera and 52 species, contaminated in tattoo inks. In this study, we investigated if these bacteria, which can survive in the azo dye-rich environment, have an ability to degrade azo dyes. METHODS: We conducted a two-step azo dye degradation (or decolorization) assay. In step 1, a high-throughput degradability assay was done for 79 bacterial isolates using Methyl Red and Orange II. In step 2, a further degradation assay was done for 10 selected bacteria with a representative of 11 azo dyes, including 3 commercial tattoo ink azo dyes. Degradation of azo dyes were calculated from measuring optical absorbance of soluble dyes at specific wavelengths. RESULTS: The initial high-throughput azo dye assay (step 1) showed that 79 isolates had a complete or partial degradation of azo dyes; > 90% of Methyl Red and Orange II were degraded within 24 h, by 74 and 20 isolates, respectively. A further evaluation of azo dye degradability for 10 selected isolates in step 2 showed that the isolates, belonging to Bacillus, Brevibacillus, Paenibacillus, and Pseudomonas, exhibited an excellent decolorization ability for a wide range of azo dyes. CONCLUSIONS: This study showed that phylogenetically diverse bacteria, isolated from azo dye-rich tattoo inks, is able to degrade a diverse range of azo dyes, including 3 azo dyes used in commercial tattoo inks. Some of the strains would be good candidates for future studies to provide a systematic understanding of azo dye degradation mechanisms.
format Online
Article
Text
id pubmed-8569640
institution National Center for Biotechnology Information
language English
publishDate 2021
record_format MEDLINE/PubMed
spelling pubmed-85696402021-11-05 Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes Nho, Seong Won Cui, Xuewen Kweon, Ohgew Jin, Jinshan Chen, Huizhong Moon, Mi Sun Kim, Seong-Jae Cerniglia, Carl E. Ann Microbiol Article PURPOSE: There has been an interest in the microbial azo dye degradation as an optional method for the treatment of azo dye-containing wastes. Tattoo ink is an extremely unique azo dye-rich environment, which have never been explored in terms of microorganisms capable of degrading azo dyes. Previously, we isolated 81 phylogenetically diverse bacteria, belonging to 18 genera and 52 species, contaminated in tattoo inks. In this study, we investigated if these bacteria, which can survive in the azo dye-rich environment, have an ability to degrade azo dyes. METHODS: We conducted a two-step azo dye degradation (or decolorization) assay. In step 1, a high-throughput degradability assay was done for 79 bacterial isolates using Methyl Red and Orange II. In step 2, a further degradation assay was done for 10 selected bacteria with a representative of 11 azo dyes, including 3 commercial tattoo ink azo dyes. Degradation of azo dyes were calculated from measuring optical absorbance of soluble dyes at specific wavelengths. RESULTS: The initial high-throughput azo dye assay (step 1) showed that 79 isolates had a complete or partial degradation of azo dyes; > 90% of Methyl Red and Orange II were degraded within 24 h, by 74 and 20 isolates, respectively. A further evaluation of azo dye degradability for 10 selected isolates in step 2 showed that the isolates, belonging to Bacillus, Brevibacillus, Paenibacillus, and Pseudomonas, exhibited an excellent decolorization ability for a wide range of azo dyes. CONCLUSIONS: This study showed that phylogenetically diverse bacteria, isolated from azo dye-rich tattoo inks, is able to degrade a diverse range of azo dyes, including 3 azo dyes used in commercial tattoo inks. Some of the strains would be good candidates for future studies to provide a systematic understanding of azo dye degradation mechanisms. 2021-09-21 /pmc/articles/PMC8569640/ /pubmed/34744534 http://dx.doi.org/10.1186/s13213-021-01648-2 Text en https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nho, Seong Won
Cui, Xuewen
Kweon, Ohgew
Jin, Jinshan
Chen, Huizhong
Moon, Mi Sun
Kim, Seong-Jae
Cerniglia, Carl E.
Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title_full Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title_fullStr Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title_full_unstemmed Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title_short Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
title_sort phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569640/
https://www.ncbi.nlm.nih.gov/pubmed/34744534
http://dx.doi.org/10.1186/s13213-021-01648-2
work_keys_str_mv AT nhoseongwon phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT cuixuewen phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT kweonohgew phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT jinjinshan phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT chenhuizhong phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT moonmisun phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT kimseongjae phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes
AT cernigliacarle phylogeneticallydiversebacteriaisolatedfromtattooinksanazodyerichenvironmentdecolorizeawiderangeofazodyes