Cargando…
Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency
The capacity of human induced pluripotent stem cells (hiPSCs) for indefinite self-renewal warrants their application in disease modeling, drug discovery, toxicity assays and efficacy screening. However, their poor proliferation ability, inability to adhere to surfaces without Matrigel coating and te...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569722/ https://www.ncbi.nlm.nih.gov/pubmed/34765963 http://dx.doi.org/10.1016/j.mtbio.2021.100153 |
_version_ | 1784594697264562176 |
---|---|
author | Liu, Yanxi Chakraborty, Shraddha Direksilp, Chatrawee Scheiger, Johannes M. Popova, Anna A. Levkin, Pavel A. |
author_facet | Liu, Yanxi Chakraborty, Shraddha Direksilp, Chatrawee Scheiger, Johannes M. Popova, Anna A. Levkin, Pavel A. |
author_sort | Liu, Yanxi |
collection | PubMed |
description | The capacity of human induced pluripotent stem cells (hiPSCs) for indefinite self-renewal warrants their application in disease modeling, drug discovery, toxicity assays and efficacy screening. However, their poor proliferation ability, inability to adhere to surfaces without Matrigel coating and tendency to spontaneously differentiate in vitro hinder the application of hiPSCs in these fields. Here we study the ability to culture hiPSCs inside 200 nL droplets on the droplet microarray (DMA) platform. We demonstrate that (1) hiPSCs can attach to the Matrigel (MG)-free surface of DMA and show good viability after 24 h culture; (2) hiPSC do not spontaneously differentiate when cultured on the MG-free surface of DMAs; (3) culturing of hiPSCs in 200 nL as compared to 2 mL culture leads to higher expression of the Nanog pluripotency marker. Overall, the results demonstrate the possibility to culture undifferentiated hiPSCs in 200 nL droplets on DMA, thereby opening the possibility for high-throughput screenings of hiPSCs with various factors without compromising the results through the involvement of animal-derived materials, such as Matrigel. |
format | Online Article Text |
id | pubmed-8569722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-85697222021-11-10 Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency Liu, Yanxi Chakraborty, Shraddha Direksilp, Chatrawee Scheiger, Johannes M. Popova, Anna A. Levkin, Pavel A. Mater Today Bio Full Length Article The capacity of human induced pluripotent stem cells (hiPSCs) for indefinite self-renewal warrants their application in disease modeling, drug discovery, toxicity assays and efficacy screening. However, their poor proliferation ability, inability to adhere to surfaces without Matrigel coating and tendency to spontaneously differentiate in vitro hinder the application of hiPSCs in these fields. Here we study the ability to culture hiPSCs inside 200 nL droplets on the droplet microarray (DMA) platform. We demonstrate that (1) hiPSCs can attach to the Matrigel (MG)-free surface of DMA and show good viability after 24 h culture; (2) hiPSC do not spontaneously differentiate when cultured on the MG-free surface of DMAs; (3) culturing of hiPSCs in 200 nL as compared to 2 mL culture leads to higher expression of the Nanog pluripotency marker. Overall, the results demonstrate the possibility to culture undifferentiated hiPSCs in 200 nL droplets on DMA, thereby opening the possibility for high-throughput screenings of hiPSCs with various factors without compromising the results through the involvement of animal-derived materials, such as Matrigel. Elsevier 2021-10-25 /pmc/articles/PMC8569722/ /pubmed/34765963 http://dx.doi.org/10.1016/j.mtbio.2021.100153 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Liu, Yanxi Chakraborty, Shraddha Direksilp, Chatrawee Scheiger, Johannes M. Popova, Anna A. Levkin, Pavel A. Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title | Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title_full | Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title_fullStr | Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title_full_unstemmed | Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title_short | Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
title_sort | miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569722/ https://www.ncbi.nlm.nih.gov/pubmed/34765963 http://dx.doi.org/10.1016/j.mtbio.2021.100153 |
work_keys_str_mv | AT liuyanxi miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency AT chakrabortyshraddha miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency AT direksilpchatrawee miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency AT scheigerjohannesm miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency AT popovaannaa miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency AT levkinpavela miniaturizeddropletmicroarrayplatformenablesmaintenanceofhumaninducedpluripotentstemcellpluripotency |