Cargando…
Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L.
Capsicum annuum L. is one of the most cultivated Solanaceae species, and in the open field, water limitation leading to drought stress affects its fruit quality, fruit setting, fruit size and ultimately yield. We identified stage-specific and a common core set of differentially expressed genes, foll...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570119/ https://www.ncbi.nlm.nih.gov/pubmed/34745217 http://dx.doi.org/10.3389/fgene.2021.743902 |
_version_ | 1784594776973115392 |
---|---|
author | Borràs, Dionis Barchi, Lorenzo Schulz, Karina Moglia, Andrea Acquadro, Alberto Kamranfar, Iman Balazadeh, Salma Lanteri, Sergio |
author_facet | Borràs, Dionis Barchi, Lorenzo Schulz, Karina Moglia, Andrea Acquadro, Alberto Kamranfar, Iman Balazadeh, Salma Lanteri, Sergio |
author_sort | Borràs, Dionis |
collection | PubMed |
description | Capsicum annuum L. is one of the most cultivated Solanaceae species, and in the open field, water limitation leading to drought stress affects its fruit quality, fruit setting, fruit size and ultimately yield. We identified stage-specific and a common core set of differentially expressed genes, following RNA-seq transcriptome analyses of a breeding line subjected to acute drought stress followed by recovery (rewatering), at three stages of plant development. Among them, two NAC transcription factor (TF) genes, i.e., CaNAC072 and CaNAC104, were always upregulated after drought stress and downregulated after recovery. The two TF proteins were observed to be localized in the nucleus following their transient expression in Nicotiana benthamiana leaves. The expression of the two NACs was also induced by NaCl, polyethylene glycol (PEG) and abscisic acid (ABA) treatments, suggesting that CaNAC072 is an early, while CaNAC104 is a late abiotic stress-responsive gene. Virus-induced gene silencing (VIGS) of CaNAC104 did not affect the pepper plantlet’s tolerance to drought stress, while VIGS of CaNAC072 increased drought tolerance. Heterologous expression of CaNAC072 in Arabidopsis thaliana as well as in plants mutated for its homolog ANAC072 did not increase drought stress tolerance. This highlights a different role of the two NAC homologs in the two species. Here, we discuss the complex role of NACs as transcriptional switches in the response to drought stress in bell pepper. |
format | Online Article Text |
id | pubmed-8570119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85701192021-11-06 Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. Borràs, Dionis Barchi, Lorenzo Schulz, Karina Moglia, Andrea Acquadro, Alberto Kamranfar, Iman Balazadeh, Salma Lanteri, Sergio Front Genet Genetics Capsicum annuum L. is one of the most cultivated Solanaceae species, and in the open field, water limitation leading to drought stress affects its fruit quality, fruit setting, fruit size and ultimately yield. We identified stage-specific and a common core set of differentially expressed genes, following RNA-seq transcriptome analyses of a breeding line subjected to acute drought stress followed by recovery (rewatering), at three stages of plant development. Among them, two NAC transcription factor (TF) genes, i.e., CaNAC072 and CaNAC104, were always upregulated after drought stress and downregulated after recovery. The two TF proteins were observed to be localized in the nucleus following their transient expression in Nicotiana benthamiana leaves. The expression of the two NACs was also induced by NaCl, polyethylene glycol (PEG) and abscisic acid (ABA) treatments, suggesting that CaNAC072 is an early, while CaNAC104 is a late abiotic stress-responsive gene. Virus-induced gene silencing (VIGS) of CaNAC104 did not affect the pepper plantlet’s tolerance to drought stress, while VIGS of CaNAC072 increased drought tolerance. Heterologous expression of CaNAC072 in Arabidopsis thaliana as well as in plants mutated for its homolog ANAC072 did not increase drought stress tolerance. This highlights a different role of the two NAC homologs in the two species. Here, we discuss the complex role of NACs as transcriptional switches in the response to drought stress in bell pepper. Frontiers Media S.A. 2021-10-22 /pmc/articles/PMC8570119/ /pubmed/34745217 http://dx.doi.org/10.3389/fgene.2021.743902 Text en Copyright © 2021 Borràs, Barchi, Schulz, Moglia, Acquadro, Kamranfar, Balazadeh and Lanteri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Borràs, Dionis Barchi, Lorenzo Schulz, Karina Moglia, Andrea Acquadro, Alberto Kamranfar, Iman Balazadeh, Salma Lanteri, Sergio Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title | Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title_full | Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title_fullStr | Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title_full_unstemmed | Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title_short | Transcriptome-Based Identification and Functional Characterization of NAC Transcription Factors Responsive to Drought Stress in Capsicum annuum L. |
title_sort | transcriptome-based identification and functional characterization of nac transcription factors responsive to drought stress in capsicum annuum l. |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570119/ https://www.ncbi.nlm.nih.gov/pubmed/34745217 http://dx.doi.org/10.3389/fgene.2021.743902 |
work_keys_str_mv | AT borrasdionis transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT barchilorenzo transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT schulzkarina transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT mogliaandrea transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT acquadroalberto transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT kamranfariman transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT balazadehsalma transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml AT lanterisergio transcriptomebasedidentificationandfunctionalcharacterizationofnactranscriptionfactorsresponsivetodroughtstressincapsicumannuuml |