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Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source
The optical design and performance of the recently opened 13A biological small-angle X-ray scattering (SAXS) beamline at the 3.0 GeV Taiwan Photon Source of the National Synchrotron Radiation Research Center are reported. The beamline is designed for studies of biological structures and kinetics in...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570220/ https://www.ncbi.nlm.nih.gov/pubmed/34738951 http://dx.doi.org/10.1107/S1600577521009565 |
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author | Liu, D.-G. Chang, C.-H. Chiang, L.-C. Lee, M.-H. Chang, C.-F. Lin, C.-Y. Liang, C.-C. Lee, T.-H. Lin, S.-W. Liu, C.-Y. Hwang, C.-S. Huang, J.-C. Kuan, C.-K. Wang, H.-S. Liu, Y.-C. Tseng, F.-H. Chuang, J.-Y. Liao, W.-R. Li, H.-C. Su, C.-J. Liao, K.-F. Yeh, Y.-Q. Shih, O. Wu, W.-R. Wang, C.-A. Jeng, U. |
author_facet | Liu, D.-G. Chang, C.-H. Chiang, L.-C. Lee, M.-H. Chang, C.-F. Lin, C.-Y. Liang, C.-C. Lee, T.-H. Lin, S.-W. Liu, C.-Y. Hwang, C.-S. Huang, J.-C. Kuan, C.-K. Wang, H.-S. Liu, Y.-C. Tseng, F.-H. Chuang, J.-Y. Liao, W.-R. Li, H.-C. Su, C.-J. Liao, K.-F. Yeh, Y.-Q. Shih, O. Wu, W.-R. Wang, C.-A. Jeng, U. |
author_sort | Liu, D.-G. |
collection | PubMed |
description | The optical design and performance of the recently opened 13A biological small-angle X-ray scattering (SAXS) beamline at the 3.0 GeV Taiwan Photon Source of the National Synchrotron Radiation Research Center are reported. The beamline is designed for studies of biological structures and kinetics in a wide range of length and time scales, from angstrom to micrometre and from microsecond to minutes. A 4 m IU24 undulator of the beamline provides high-flux X-rays in the energy range 4.0–23.0 keV. MoB(4)C double-multilayer and Si(111) double-crystal monochromators (DMM/DCM) are combined on the same rotating platform for a smooth rotation transition from a high-flux beam of ∼4 × 10(14) photons s(−1) to a high-energy-resolution beam of ΔE/E ≃ 1.5 × 10(−4); both modes share a constant beam exit. With a set of Kirkpatrick–Baez (KB) mirrors, the X-ray beam is focused to the farthest SAXS detector position, 52 m from the source. A downstream four-bounce crystal collimator, comprising two sets of Si(311) double crystals arranged in a dispersive configuration, optionally collimate the DCM (vertically diffracted) beam in the horizontal direction for ultra-SAXS with a minimum scattering vector q down to 0.0004 Å(−1), which allows resolving ordered d-spacing up to 1 µm. A microbeam, of 10–50 µm beam size, is tailored by a combined set of high-heat-load slits followed by micrometre-precision slits situated at the front-end 15.5 m position. The second set of KB mirrors then focus the beam to the 40 m sample position, with a demagnification ratio of ∼1.5. A detecting system comprising two in-vacuum X-ray pixel detectors is installed to perform synchronized small- and wide-angle X-ray scattering data collections. The observed beamline performance proves the feasibility of having compound features of high flux, microbeam and ultra-SAXS in one beamline. |
format | Online Article Text |
id | pubmed-8570220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-85702202021-11-18 Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source Liu, D.-G. Chang, C.-H. Chiang, L.-C. Lee, M.-H. Chang, C.-F. Lin, C.-Y. Liang, C.-C. Lee, T.-H. Lin, S.-W. Liu, C.-Y. Hwang, C.-S. Huang, J.-C. Kuan, C.-K. Wang, H.-S. Liu, Y.-C. Tseng, F.-H. Chuang, J.-Y. Liao, W.-R. Li, H.-C. Su, C.-J. Liao, K.-F. Yeh, Y.-Q. Shih, O. Wu, W.-R. Wang, C.-A. Jeng, U. J Synchrotron Radiat Beamlines The optical design and performance of the recently opened 13A biological small-angle X-ray scattering (SAXS) beamline at the 3.0 GeV Taiwan Photon Source of the National Synchrotron Radiation Research Center are reported. The beamline is designed for studies of biological structures and kinetics in a wide range of length and time scales, from angstrom to micrometre and from microsecond to minutes. A 4 m IU24 undulator of the beamline provides high-flux X-rays in the energy range 4.0–23.0 keV. MoB(4)C double-multilayer and Si(111) double-crystal monochromators (DMM/DCM) are combined on the same rotating platform for a smooth rotation transition from a high-flux beam of ∼4 × 10(14) photons s(−1) to a high-energy-resolution beam of ΔE/E ≃ 1.5 × 10(−4); both modes share a constant beam exit. With a set of Kirkpatrick–Baez (KB) mirrors, the X-ray beam is focused to the farthest SAXS detector position, 52 m from the source. A downstream four-bounce crystal collimator, comprising two sets of Si(311) double crystals arranged in a dispersive configuration, optionally collimate the DCM (vertically diffracted) beam in the horizontal direction for ultra-SAXS with a minimum scattering vector q down to 0.0004 Å(−1), which allows resolving ordered d-spacing up to 1 µm. A microbeam, of 10–50 µm beam size, is tailored by a combined set of high-heat-load slits followed by micrometre-precision slits situated at the front-end 15.5 m position. The second set of KB mirrors then focus the beam to the 40 m sample position, with a demagnification ratio of ∼1.5. A detecting system comprising two in-vacuum X-ray pixel detectors is installed to perform synchronized small- and wide-angle X-ray scattering data collections. The observed beamline performance proves the feasibility of having compound features of high flux, microbeam and ultra-SAXS in one beamline. International Union of Crystallography 2021-10-18 /pmc/articles/PMC8570220/ /pubmed/34738951 http://dx.doi.org/10.1107/S1600577521009565 Text en © D.-G. Liu et al. 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Beamlines Liu, D.-G. Chang, C.-H. Chiang, L.-C. Lee, M.-H. Chang, C.-F. Lin, C.-Y. Liang, C.-C. Lee, T.-H. Lin, S.-W. Liu, C.-Y. Hwang, C.-S. Huang, J.-C. Kuan, C.-K. Wang, H.-S. Liu, Y.-C. Tseng, F.-H. Chuang, J.-Y. Liao, W.-R. Li, H.-C. Su, C.-J. Liao, K.-F. Yeh, Y.-Q. Shih, O. Wu, W.-R. Wang, C.-A. Jeng, U. Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title | Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title_full | Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title_fullStr | Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title_full_unstemmed | Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title_short | Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source |
title_sort | optical design and performance of the biological small-angle x-ray scattering beamline at the taiwan photon source |
topic | Beamlines |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570220/ https://www.ncbi.nlm.nih.gov/pubmed/34738951 http://dx.doi.org/10.1107/S1600577521009565 |
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