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Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells

The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithelial polarity are beginning to be unraveled, its roles in oth...

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Autores principales: Riga, Amalia, Cravo, Janine, Schmidt, Ruben, Pires, Helena R., Castiglioni, Victoria G., van den Heuvel, Sander, Boxem, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570498/
https://www.ncbi.nlm.nih.gov/pubmed/34673778
http://dx.doi.org/10.1371/journal.pgen.1009856
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author Riga, Amalia
Cravo, Janine
Schmidt, Ruben
Pires, Helena R.
Castiglioni, Victoria G.
van den Heuvel, Sander
Boxem, Mike
author_facet Riga, Amalia
Cravo, Janine
Schmidt, Ruben
Pires, Helena R.
Castiglioni, Victoria G.
van den Heuvel, Sander
Boxem, Mike
author_sort Riga, Amalia
collection PubMed
description The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithelial polarity are beginning to be unraveled, its roles in other cellular processes including cell migration remain enigmatic. In C. elegans, the Scrib ortholog LET-413 is essential for apical–basal polarization and junction formation in embryonic epithelia. However, whether LET-413 is required for postembryonic development or plays a role in migratory events is not known. Here, we use inducible protein degradation to investigate the functioning of LET-413 in larval epithelia. We find that LET-413 is essential in the epidermal epithelium for growth, viability, and junction maintenance. In addition, we identify a novel role for LET-413 in the polarized outgrowth of the epidermal seam cells. These stem cell-like epithelial cells extend anterior and posterior directed apical protrusions in each larval stage to reconnect to their neighbors. We show that the role of LET-413 in seam cell outgrowth is likely mediated largely by the junctional component DLG-1 discs large, which we demonstrate is also essential for directed outgrowth of the seam cells. Our data uncover multiple essential functions for LET-413 in larval development and show that the polarized outgrowth of the epithelial seam cells is controlled by LET-413 Scribble and DLG-1 Discs large.
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spelling pubmed-85704982021-11-06 Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells Riga, Amalia Cravo, Janine Schmidt, Ruben Pires, Helena R. Castiglioni, Victoria G. van den Heuvel, Sander Boxem, Mike PLoS Genet Research Article The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithelial polarity are beginning to be unraveled, its roles in other cellular processes including cell migration remain enigmatic. In C. elegans, the Scrib ortholog LET-413 is essential for apical–basal polarization and junction formation in embryonic epithelia. However, whether LET-413 is required for postembryonic development or plays a role in migratory events is not known. Here, we use inducible protein degradation to investigate the functioning of LET-413 in larval epithelia. We find that LET-413 is essential in the epidermal epithelium for growth, viability, and junction maintenance. In addition, we identify a novel role for LET-413 in the polarized outgrowth of the epidermal seam cells. These stem cell-like epithelial cells extend anterior and posterior directed apical protrusions in each larval stage to reconnect to their neighbors. We show that the role of LET-413 in seam cell outgrowth is likely mediated largely by the junctional component DLG-1 discs large, which we demonstrate is also essential for directed outgrowth of the seam cells. Our data uncover multiple essential functions for LET-413 in larval development and show that the polarized outgrowth of the epithelial seam cells is controlled by LET-413 Scribble and DLG-1 Discs large. Public Library of Science 2021-10-21 /pmc/articles/PMC8570498/ /pubmed/34673778 http://dx.doi.org/10.1371/journal.pgen.1009856 Text en © 2021 Riga et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Riga, Amalia
Cravo, Janine
Schmidt, Ruben
Pires, Helena R.
Castiglioni, Victoria G.
van den Heuvel, Sander
Boxem, Mike
Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title_full Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title_fullStr Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title_full_unstemmed Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title_short Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
title_sort caenorhabditis elegans let-413 scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570498/
https://www.ncbi.nlm.nih.gov/pubmed/34673778
http://dx.doi.org/10.1371/journal.pgen.1009856
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