Cargando…
3D cell neighbour dynamics in growing pseudostratified epithelia
During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell seg...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570695/ https://www.ncbi.nlm.nih.gov/pubmed/34609280 http://dx.doi.org/10.7554/eLife.68135 |
_version_ | 1784594881278115840 |
---|---|
author | Gómez, Harold Fernando Dumond, Mathilde Sabine Hodel, Leonie Vetter, Roman Iber, Dagmar |
author_facet | Gómez, Harold Fernando Dumond, Mathilde Sabine Hodel, Leonie Vetter, Roman Iber, Dagmar |
author_sort | Gómez, Harold Fernando |
collection | PubMed |
description | During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell segmentation, and physical theory to unravel the principles behind 3D cell organisation in growing pseudostratified epithelia. We find that cells have highly irregular 3D shapes and exhibit numerous neighbour intercalations along the apical-basal axis as well as over time. Despite the fluidic nature, the cell packing configurations follow fundamental relationships previously described for apical epithelial layers, that is, Euler's polyhedron formula, Lewis’ law, and Aboav-Weaire's law, at all times and across the entire tissue thickness. This arrangement minimises the lateral cell-cell surface energy for a given cross-sectional area variability, generated primarily by the distribution and movement of nuclei. We conclude that the complex 3D cell organisation in growing epithelia emerges from simple physical principles. |
format | Online Article Text |
id | pubmed-8570695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-85706952021-11-08 3D cell neighbour dynamics in growing pseudostratified epithelia Gómez, Harold Fernando Dumond, Mathilde Sabine Hodel, Leonie Vetter, Roman Iber, Dagmar eLife Cell Biology During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell segmentation, and physical theory to unravel the principles behind 3D cell organisation in growing pseudostratified epithelia. We find that cells have highly irregular 3D shapes and exhibit numerous neighbour intercalations along the apical-basal axis as well as over time. Despite the fluidic nature, the cell packing configurations follow fundamental relationships previously described for apical epithelial layers, that is, Euler's polyhedron formula, Lewis’ law, and Aboav-Weaire's law, at all times and across the entire tissue thickness. This arrangement minimises the lateral cell-cell surface energy for a given cross-sectional area variability, generated primarily by the distribution and movement of nuclei. We conclude that the complex 3D cell organisation in growing epithelia emerges from simple physical principles. eLife Sciences Publications, Ltd 2021-10-05 /pmc/articles/PMC8570695/ /pubmed/34609280 http://dx.doi.org/10.7554/eLife.68135 Text en © 2021, Gómez et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Gómez, Harold Fernando Dumond, Mathilde Sabine Hodel, Leonie Vetter, Roman Iber, Dagmar 3D cell neighbour dynamics in growing pseudostratified epithelia |
title | 3D cell neighbour dynamics in growing pseudostratified epithelia |
title_full | 3D cell neighbour dynamics in growing pseudostratified epithelia |
title_fullStr | 3D cell neighbour dynamics in growing pseudostratified epithelia |
title_full_unstemmed | 3D cell neighbour dynamics in growing pseudostratified epithelia |
title_short | 3D cell neighbour dynamics in growing pseudostratified epithelia |
title_sort | 3d cell neighbour dynamics in growing pseudostratified epithelia |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570695/ https://www.ncbi.nlm.nih.gov/pubmed/34609280 http://dx.doi.org/10.7554/eLife.68135 |
work_keys_str_mv | AT gomezharoldfernando 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT dumondmathildesabine 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT hodelleonie 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT vetterroman 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia AT iberdagmar 3dcellneighbourdynamicsingrowingpseudostratifiedepithelia |