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Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction

The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel...

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Autores principales: Kubota, Masaaki, Yoshida, Yoichi, Kobayashi, Eiichi, Matsutani, Tomoo, Li, Shu-Yang, Zhang, Bo-Shi, Mine, Seiichiro, Machida, Toshio, Takizawa, Hirotaka, Hiwasa, Takaki, Iwadate, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571331/
https://www.ncbi.nlm.nih.gov/pubmed/34741085
http://dx.doi.org/10.1038/s41598-021-01176-8
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author Kubota, Masaaki
Yoshida, Yoichi
Kobayashi, Eiichi
Matsutani, Tomoo
Li, Shu-Yang
Zhang, Bo-Shi
Mine, Seiichiro
Machida, Toshio
Takizawa, Hirotaka
Hiwasa, Takaki
Iwadate, Yasuo
author_facet Kubota, Masaaki
Yoshida, Yoichi
Kobayashi, Eiichi
Matsutani, Tomoo
Li, Shu-Yang
Zhang, Bo-Shi
Mine, Seiichiro
Machida, Toshio
Takizawa, Hirotaka
Hiwasa, Takaki
Iwadate, Yasuo
author_sort Kubota, Masaaki
collection PubMed
description The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction.
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spelling pubmed-85713312021-11-09 Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction Kubota, Masaaki Yoshida, Yoichi Kobayashi, Eiichi Matsutani, Tomoo Li, Shu-Yang Zhang, Bo-Shi Mine, Seiichiro Machida, Toshio Takizawa, Hirotaka Hiwasa, Takaki Iwadate, Yasuo Sci Rep Article The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction. Nature Publishing Group UK 2021-11-05 /pmc/articles/PMC8571331/ /pubmed/34741085 http://dx.doi.org/10.1038/s41598-021-01176-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kubota, Masaaki
Yoshida, Yoichi
Kobayashi, Eiichi
Matsutani, Tomoo
Li, Shu-Yang
Zhang, Bo-Shi
Mine, Seiichiro
Machida, Toshio
Takizawa, Hirotaka
Hiwasa, Takaki
Iwadate, Yasuo
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title_full Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title_fullStr Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title_full_unstemmed Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title_short Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
title_sort serum anti-serpine1 antibody as a potential biomarker of acute cerebral infarction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571331/
https://www.ncbi.nlm.nih.gov/pubmed/34741085
http://dx.doi.org/10.1038/s41598-021-01176-8
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