Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division

This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can b...

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Detalles Bibliográficos
Autores principales: Belmonte, Miriam, Kent, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571517/
https://www.ncbi.nlm.nih.gov/pubmed/34766031
http://dx.doi.org/10.1016/j.xpro.2021.100927
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author Belmonte, Miriam
Kent, David G.
author_facet Belmonte, Miriam
Kent, David G.
author_sort Belmonte, Miriam
collection PubMed
description This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can be used to study quiescence exit and can be genetically manipulated as single cells prior to division. For complete details on the use and execution of this protocol, please refer to Oedekoven et al. (2021).
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spelling pubmed-85715172021-11-10 Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division Belmonte, Miriam Kent, David G. STAR Protoc Protocol This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can be used to study quiescence exit and can be genetically manipulated as single cells prior to division. For complete details on the use and execution of this protocol, please refer to Oedekoven et al. (2021). Elsevier 2021-11-01 /pmc/articles/PMC8571517/ /pubmed/34766031 http://dx.doi.org/10.1016/j.xpro.2021.100927 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Belmonte, Miriam
Kent, David G.
Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title_full Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title_fullStr Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title_full_unstemmed Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title_short Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
title_sort protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571517/
https://www.ncbi.nlm.nih.gov/pubmed/34766031
http://dx.doi.org/10.1016/j.xpro.2021.100927
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