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Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division

This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can b...

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Detalles Bibliográficos
Autores principales:	Belmonte, Miriam, Kent, David G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571517/ https://www.ncbi.nlm.nih.gov/pubmed/34766031 http://dx.doi.org/10.1016/j.xpro.2021.100927

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