Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro

The regulation of lipid kinases has remained elusive given the difficulties of assessing changes in lipid levels. Here, we describe the isolation of protein and lipid kinases to determine the regulation of lipid kinases in vitro. This can be followed by analysis of effects of regulators on lipid kin...

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Detalles Bibliográficos
Autores principales: Karabiyik, Cansu, Rubinsztein, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571518/
https://www.ncbi.nlm.nih.gov/pubmed/34766030
http://dx.doi.org/10.1016/j.xpro.2021.100926
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author Karabiyik, Cansu
Rubinsztein, David C.
author_facet Karabiyik, Cansu
Rubinsztein, David C.
author_sort Karabiyik, Cansu
collection PubMed
description The regulation of lipid kinases has remained elusive given the difficulties of assessing changes in lipid levels. Here, we describe the isolation of protein and lipid kinases to determine the regulation of lipid kinases in vitro. This can be followed by analysis of effects of regulators on lipid kinase-mediated changes in phospholipids without the use of radioactivity, with a specific focus on PI(5)P generation by the enzyme PIKfyve. For complete details on the use and execution of this protocol, please refer to Karabiyik et al. (2021).
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spelling pubmed-85715182021-11-10 Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro Karabiyik, Cansu Rubinsztein, David C. STAR Protoc Protocol The regulation of lipid kinases has remained elusive given the difficulties of assessing changes in lipid levels. Here, we describe the isolation of protein and lipid kinases to determine the regulation of lipid kinases in vitro. This can be followed by analysis of effects of regulators on lipid kinase-mediated changes in phospholipids without the use of radioactivity, with a specific focus on PI(5)P generation by the enzyme PIKfyve. For complete details on the use and execution of this protocol, please refer to Karabiyik et al. (2021). Elsevier 2021-11-01 /pmc/articles/PMC8571518/ /pubmed/34766030 http://dx.doi.org/10.1016/j.xpro.2021.100926 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Karabiyik, Cansu
Rubinsztein, David C.
Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title_full Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title_fullStr Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title_full_unstemmed Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title_short Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
title_sort protocol for determining the regulation of lipid kinases and changes in phospholipids in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571518/
https://www.ncbi.nlm.nih.gov/pubmed/34766030
http://dx.doi.org/10.1016/j.xpro.2021.100926
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