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The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions
The basophil activation test (BAT) is an ex vivo functional assay that measures by flow cytometry the degree of basophil degranulation after stimulation with an allergen. In recent years, there has been an increased interest in the diagnostic value of the BAT as it has the potential to mimic the cli...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572092/ https://www.ncbi.nlm.nih.gov/pubmed/34754200 http://dx.doi.org/10.2147/JAA.S237759 |
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author | Briceno Noriega, Daniela Teodorowicz, Malgorzata Savelkoul, Huub Ruinemans-Koerts, Janneke |
author_facet | Briceno Noriega, Daniela Teodorowicz, Malgorzata Savelkoul, Huub Ruinemans-Koerts, Janneke |
author_sort | Briceno Noriega, Daniela |
collection | PubMed |
description | The basophil activation test (BAT) is an ex vivo functional assay that measures by flow cytometry the degree of basophil degranulation after stimulation with an allergen. In recent years, there has been an increased interest in the diagnostic value of the BAT as it has the potential to mimic the clinical phenotype of sIgE sensitized patients, in contrast to allergen-specific IgE levels. This diagnostic potential would be of particular interest for food allergies present early in life such as peanut, cow’s milk and eggs, which require an expensive, time-consuming and patient unfriendly oral food challenge (OFC) for diagnosis. However, routine applications of the BAT for clinical use are not yet feasible due to the lack of standardized protocols and large clinical validation studies. This review will summarize the current data regarding the application of the BAT in food allergy (FA) for cow’s milk, egg and peanut, being the most common causes of FA in children. Additionally, it will discuss the hurdles for widespread clinical use of the BAT and possible future directions for this diagnostic procedure. |
format | Online Article Text |
id | pubmed-8572092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-85720922021-11-08 The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions Briceno Noriega, Daniela Teodorowicz, Malgorzata Savelkoul, Huub Ruinemans-Koerts, Janneke J Asthma Allergy Review The basophil activation test (BAT) is an ex vivo functional assay that measures by flow cytometry the degree of basophil degranulation after stimulation with an allergen. In recent years, there has been an increased interest in the diagnostic value of the BAT as it has the potential to mimic the clinical phenotype of sIgE sensitized patients, in contrast to allergen-specific IgE levels. This diagnostic potential would be of particular interest for food allergies present early in life such as peanut, cow’s milk and eggs, which require an expensive, time-consuming and patient unfriendly oral food challenge (OFC) for diagnosis. However, routine applications of the BAT for clinical use are not yet feasible due to the lack of standardized protocols and large clinical validation studies. This review will summarize the current data regarding the application of the BAT in food allergy (FA) for cow’s milk, egg and peanut, being the most common causes of FA in children. Additionally, it will discuss the hurdles for widespread clinical use of the BAT and possible future directions for this diagnostic procedure. Dove 2021-11-02 /pmc/articles/PMC8572092/ /pubmed/34754200 http://dx.doi.org/10.2147/JAA.S237759 Text en © 2021 Briceno Noriega et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Review Briceno Noriega, Daniela Teodorowicz, Malgorzata Savelkoul, Huub Ruinemans-Koerts, Janneke The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title | The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title_full | The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title_fullStr | The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title_full_unstemmed | The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title_short | The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions |
title_sort | basophil activation test for clinical management of food allergies: recent advances and future directions |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572092/ https://www.ncbi.nlm.nih.gov/pubmed/34754200 http://dx.doi.org/10.2147/JAA.S237759 |
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