Cargando…
Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
INTRODUCTION: Human decidua basalis mesenchymal stem cells (DBMSCs) are potential therapeutics for the medication to cure inflammatory diseases, like atherosclerosis. The current study investigates the capacity of DBMSCs to stay alive and function in a harmful inflammatory environment induced by hig...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572118/ https://www.ncbi.nlm.nih.gov/pubmed/34754198 http://dx.doi.org/10.2147/SCCAA.S332952 |
_version_ | 1784595163565260800 |
---|---|
author | Alshareef, Ghofran Hasan Mohammed, Afrah E Abumaree, Mohammed Basmaeil, Yasser S |
author_facet | Alshareef, Ghofran Hasan Mohammed, Afrah E Abumaree, Mohammed Basmaeil, Yasser S |
author_sort | Alshareef, Ghofran Hasan |
collection | PubMed |
description | INTRODUCTION: Human decidua basalis mesenchymal stem cells (DBMSCs) are potential therapeutics for the medication to cure inflammatory diseases, like atherosclerosis. The current study investigates the capacity of DBMSCs to stay alive and function in a harmful inflammatory environment induced by high levels of lipopolysaccharide (LPS). METHODS: DBMSCs were exposed to different levels of LPS, and their viability and functional responses (proliferation, adhesion, and migration) were examined. Furthermore, DBMSCs’ expression of 84 genes associated with their functional activities in the presence of LPS was investigated. RESULTS: Results indicated that LPS had no significant effect on DBMSCs’ adhesion, migration, and proliferation (24 h and 72 h) (p > 0.05). However, DBMSCs’ proliferation was significantly reduced at 10 µg/mL of LPS at 48 h (p < 0.05). In addition, inflammatory cytokines and receptors related to adhesion, proliferation, migration, and differentiation were significantly overexpressed when DBMSCs were treated with 10 µg/mL of LPS (p < 0.05). CONCLUSION: These results indicated that DBMSCs maintained their functional activities (proliferation, adhesion, and migration) in the presence of LPS as there was no variation between the treated DBMSCs and the control group. This study will lay the foundation for future preclinical and clinical studies to confirm the appropriateness of DBMSCs as a potential medication to cure inflammatory diseases, like atherosclerosis. |
format | Online Article Text |
id | pubmed-8572118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-85721182021-11-08 Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria Alshareef, Ghofran Hasan Mohammed, Afrah E Abumaree, Mohammed Basmaeil, Yasser S Stem Cells Cloning Original Research INTRODUCTION: Human decidua basalis mesenchymal stem cells (DBMSCs) are potential therapeutics for the medication to cure inflammatory diseases, like atherosclerosis. The current study investigates the capacity of DBMSCs to stay alive and function in a harmful inflammatory environment induced by high levels of lipopolysaccharide (LPS). METHODS: DBMSCs were exposed to different levels of LPS, and their viability and functional responses (proliferation, adhesion, and migration) were examined. Furthermore, DBMSCs’ expression of 84 genes associated with their functional activities in the presence of LPS was investigated. RESULTS: Results indicated that LPS had no significant effect on DBMSCs’ adhesion, migration, and proliferation (24 h and 72 h) (p > 0.05). However, DBMSCs’ proliferation was significantly reduced at 10 µg/mL of LPS at 48 h (p < 0.05). In addition, inflammatory cytokines and receptors related to adhesion, proliferation, migration, and differentiation were significantly overexpressed when DBMSCs were treated with 10 µg/mL of LPS (p < 0.05). CONCLUSION: These results indicated that DBMSCs maintained their functional activities (proliferation, adhesion, and migration) in the presence of LPS as there was no variation between the treated DBMSCs and the control group. This study will lay the foundation for future preclinical and clinical studies to confirm the appropriateness of DBMSCs as a potential medication to cure inflammatory diseases, like atherosclerosis. Dove 2021-11-02 /pmc/articles/PMC8572118/ /pubmed/34754198 http://dx.doi.org/10.2147/SCCAA.S332952 Text en © 2021 Alshareef et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Alshareef, Ghofran Hasan Mohammed, Afrah E Abumaree, Mohammed Basmaeil, Yasser S Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title | Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title_full | Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title_fullStr | Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title_full_unstemmed | Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title_short | Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria |
title_sort | phenotypic and functional responses of human decidua basalis mesenchymal stem/stromal cells to lipopolysaccharide of gram-negative bacteria |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572118/ https://www.ncbi.nlm.nih.gov/pubmed/34754198 http://dx.doi.org/10.2147/SCCAA.S332952 |
work_keys_str_mv | AT alshareefghofranhasan phenotypicandfunctionalresponsesofhumandeciduabasalismesenchymalstemstromalcellstolipopolysaccharideofgramnegativebacteria AT mohammedafrahe phenotypicandfunctionalresponsesofhumandeciduabasalismesenchymalstemstromalcellstolipopolysaccharideofgramnegativebacteria AT abumareemohammed phenotypicandfunctionalresponsesofhumandeciduabasalismesenchymalstemstromalcellstolipopolysaccharideofgramnegativebacteria AT basmaeilyassers phenotypicandfunctionalresponsesofhumandeciduabasalismesenchymalstemstromalcellstolipopolysaccharideofgramnegativebacteria |