Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential

PURPOSE: Brewers’ spent grain (BSG) represents the largest by-product of the brewing industry. Its utilisation as an animal feed has become less practical today; however, its high fibre and protein content make it a promising untapped resource for human nutrition. BSG contains mainly insoluble fibre...

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Autores principales: Lynch, Kieran M., Strain, Conall R., Johnson, Crystal, Patangia, Dhrati, Stanton, Catherine, Koc, Fatma, Gil-Martinez, Jorge, O’Riordan, Patrick, Sahin, Aylin W., Ross, R. Paul, Arendt, Elke K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572209/
https://www.ncbi.nlm.nih.gov/pubmed/34057578
http://dx.doi.org/10.1007/s00394-021-02570-8
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author Lynch, Kieran M.
Strain, Conall R.
Johnson, Crystal
Patangia, Dhrati
Stanton, Catherine
Koc, Fatma
Gil-Martinez, Jorge
O’Riordan, Patrick
Sahin, Aylin W.
Ross, R. Paul
Arendt, Elke K.
author_facet Lynch, Kieran M.
Strain, Conall R.
Johnson, Crystal
Patangia, Dhrati
Stanton, Catherine
Koc, Fatma
Gil-Martinez, Jorge
O’Riordan, Patrick
Sahin, Aylin W.
Ross, R. Paul
Arendt, Elke K.
author_sort Lynch, Kieran M.
collection PubMed
description PURPOSE: Brewers’ spent grain (BSG) represents the largest by-product of the brewing industry. Its utilisation as an animal feed has become less practical today; however, its high fibre and protein content make it a promising untapped resource for human nutrition. BSG contains mainly insoluble fibre. This fibre, along with protein, is trapped with the complex lignocellulosic cell structure and must be solubilised to release components which may be beneficial to health through modulation of the gut microbiota. METHODS: In this study, the application of a simultaneous saccharification and fermentation process for the extraction and solubilisation of arabinoxylan from BSG is demonstrated. RESULTS: Processing of the BSG was varied to modulate the physicochemical and molecular characteristic of the released arabinoxylan. The maximum level of arabinoxylan solubilisation achieved was approximately 21%, compared to the unprocessed BSG which contained no soluble arabinoxylan (AX). Concentration of the solubilised material produced a sample containing 99% soluble AX. Samples were investigated for their microbiome modulating capacity in in-vitro faecal fermentation trials. Many samples promoted increased Lactobacillus levels (approx. twofold). One sample that contained the highest level of soluble AX was shown to be bifidogenic, increasing the levels of this genus approx. 3.5-fold as well as acetate (p = 0.018) and propionate (p < 0.001) production. CONCLUSION: The findings indicate that AX extracted from BSG has prebiotic potential. The demonstration that BSG is a source of functional fibre is a promising step towards the application of this brewing side-stream as a functional food ingredient for human nutrition. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00394-021-02570-8.
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spelling pubmed-85722092021-11-15 Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential Lynch, Kieran M. Strain, Conall R. Johnson, Crystal Patangia, Dhrati Stanton, Catherine Koc, Fatma Gil-Martinez, Jorge O’Riordan, Patrick Sahin, Aylin W. Ross, R. Paul Arendt, Elke K. Eur J Nutr Original Contribution PURPOSE: Brewers’ spent grain (BSG) represents the largest by-product of the brewing industry. Its utilisation as an animal feed has become less practical today; however, its high fibre and protein content make it a promising untapped resource for human nutrition. BSG contains mainly insoluble fibre. This fibre, along with protein, is trapped with the complex lignocellulosic cell structure and must be solubilised to release components which may be beneficial to health through modulation of the gut microbiota. METHODS: In this study, the application of a simultaneous saccharification and fermentation process for the extraction and solubilisation of arabinoxylan from BSG is demonstrated. RESULTS: Processing of the BSG was varied to modulate the physicochemical and molecular characteristic of the released arabinoxylan. The maximum level of arabinoxylan solubilisation achieved was approximately 21%, compared to the unprocessed BSG which contained no soluble arabinoxylan (AX). Concentration of the solubilised material produced a sample containing 99% soluble AX. Samples were investigated for their microbiome modulating capacity in in-vitro faecal fermentation trials. Many samples promoted increased Lactobacillus levels (approx. twofold). One sample that contained the highest level of soluble AX was shown to be bifidogenic, increasing the levels of this genus approx. 3.5-fold as well as acetate (p = 0.018) and propionate (p < 0.001) production. CONCLUSION: The findings indicate that AX extracted from BSG has prebiotic potential. The demonstration that BSG is a source of functional fibre is a promising step towards the application of this brewing side-stream as a functional food ingredient for human nutrition. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00394-021-02570-8. Springer Berlin Heidelberg 2021-05-31 2021 /pmc/articles/PMC8572209/ /pubmed/34057578 http://dx.doi.org/10.1007/s00394-021-02570-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Contribution
Lynch, Kieran M.
Strain, Conall R.
Johnson, Crystal
Patangia, Dhrati
Stanton, Catherine
Koc, Fatma
Gil-Martinez, Jorge
O’Riordan, Patrick
Sahin, Aylin W.
Ross, R. Paul
Arendt, Elke K.
Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title_full Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title_fullStr Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title_full_unstemmed Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title_short Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
title_sort extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572209/
https://www.ncbi.nlm.nih.gov/pubmed/34057578
http://dx.doi.org/10.1007/s00394-021-02570-8
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