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Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis

BACKGROUND: Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. T...

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Autores principales: Li, Qiang, Liu, Guanlin, Cheng, Yue, Tang, Wenbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572504/
https://www.ncbi.nlm.nih.gov/pubmed/34742263
http://dx.doi.org/10.1186/s12894-021-00914-4
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author Li, Qiang
Liu, Guanlin
Cheng, Yue
Tang, Wenbo
author_facet Li, Qiang
Liu, Guanlin
Cheng, Yue
Tang, Wenbo
author_sort Li, Qiang
collection PubMed
description BACKGROUND: Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. The fact that different urinary stone-related compounds (USRCs) are measured by different methods renders the metabolic evaluation of urolithiasis quite tedious and complex. METHODS: A three-channel ion chromatograph (IC) that automatically measures the concentration of common metabolic indicators of urolithiasis in urine (i.e., oxalate, citrate, uric acid, calcium, and magnesium) was developed to improve the efficiency. To validate its precision and specificity, standard curves were prepared using working solution of these indicators. 100 standard solutions of these indicators were measured with our new IC and three other ICs as the control instruments; analyte concentrations in 100 24-h urine samples from volunteers and 135 calculi patients were also measured. RESULTS: All analytes had good linear relationships in concentration ranges of 0–10 mg/L. The precision experiments in the standard and urine samples showed that the measurement errors of the newly developed IC were all less than 5%. In urine, the recovery rate ranged from 99.6 to 100.4%, the coefficient of variation ranged from 1.39 to 2.99%, and the results matched between our newly developed IC and the control ICs. The results of the efficiency test showed that we can finish the analysis at the average number of 14 people per day with the new IC. While the average number in the control group is 3.85/day (p = 0.000). CONCLUSIONS: Overall, this multi-channel system significantly improves the efficiency of metabolic evaluation while retaining accuracy and precision. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-021-00914-4.
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spelling pubmed-85725042021-11-08 Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis Li, Qiang Liu, Guanlin Cheng, Yue Tang, Wenbo BMC Urol Research Article BACKGROUND: Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. The fact that different urinary stone-related compounds (USRCs) are measured by different methods renders the metabolic evaluation of urolithiasis quite tedious and complex. METHODS: A three-channel ion chromatograph (IC) that automatically measures the concentration of common metabolic indicators of urolithiasis in urine (i.e., oxalate, citrate, uric acid, calcium, and magnesium) was developed to improve the efficiency. To validate its precision and specificity, standard curves were prepared using working solution of these indicators. 100 standard solutions of these indicators were measured with our new IC and three other ICs as the control instruments; analyte concentrations in 100 24-h urine samples from volunteers and 135 calculi patients were also measured. RESULTS: All analytes had good linear relationships in concentration ranges of 0–10 mg/L. The precision experiments in the standard and urine samples showed that the measurement errors of the newly developed IC were all less than 5%. In urine, the recovery rate ranged from 99.6 to 100.4%, the coefficient of variation ranged from 1.39 to 2.99%, and the results matched between our newly developed IC and the control ICs. The results of the efficiency test showed that we can finish the analysis at the average number of 14 people per day with the new IC. While the average number in the control group is 3.85/day (p = 0.000). CONCLUSIONS: Overall, this multi-channel system significantly improves the efficiency of metabolic evaluation while retaining accuracy and precision. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12894-021-00914-4. BioMed Central 2021-11-06 /pmc/articles/PMC8572504/ /pubmed/34742263 http://dx.doi.org/10.1186/s12894-021-00914-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Li, Qiang
Liu, Guanlin
Cheng, Yue
Tang, Wenbo
Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_full Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_fullStr Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_full_unstemmed Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_short Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_sort three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572504/
https://www.ncbi.nlm.nih.gov/pubmed/34742263
http://dx.doi.org/10.1186/s12894-021-00914-4
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