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Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis
Fruit aromas are composed of a complex mixture of volatile organic compounds, which are essential attributes associated with the overall flavor and consumer preference. Muscadine grape (MG; Vitis rotundifolia Michx.) is an aroma-dense fruit crop. However, there is very scarce information on its vola...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572961/ https://www.ncbi.nlm.nih.gov/pubmed/34759938 http://dx.doi.org/10.3389/fpls.2021.728891 |
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author | Deng, Honghong He, Runmei Long, Meicun Li, Yanmei Zheng, Yuanyuan Lin, Lijin Liang, Dong Zhang, Xiaoai Liao, Ming'an Lv, Xiulan Deng, Qunxian Xia, Hui |
author_facet | Deng, Honghong He, Runmei Long, Meicun Li, Yanmei Zheng, Yuanyuan Lin, Lijin Liang, Dong Zhang, Xiaoai Liao, Ming'an Lv, Xiulan Deng, Qunxian Xia, Hui |
author_sort | Deng, Honghong |
collection | PubMed |
description | Fruit aromas are composed of a complex mixture of volatile organic compounds, which are essential attributes associated with the overall flavor and consumer preference. Muscadine grape (MG; Vitis rotundifolia Michx.) is an aroma-dense fruit crop. However, there is very scarce information on its volatile profiles. In this study, the volatile constituents of five newly introduced MG cultivars, including Alachua, Carlos, Fry, Granny Val, and Noble, were profiled using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC/MS) combined with multivariate statistical analysis. A total of 44 compounds, including esters, aldehydes, alcohols, fatty acids, terpenes, ketones, and furan, were identified and relatively quantified. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) evidently discriminated against the five MG cultivars based on their volatile profiles. The specific volatiles that contributed the most to this discrimination were highlighted. Geraniol and cinnamyl alcohol were demonstrated to be essential for characterizing the Alachua MG cultivar, whereas ethyl trans-2-butenoate and propyl acetate were shown to be important compounds to characterize the Noble MG cultivar. The results further showed that 2-Ethyl-1-hexanol, (Z)-3-hexenal, and (E)-2-hexenol were closely related to Carlos, Fry, and Granny Val cultivars, respectively. This investigation is the first in-depth exploration of the volatile profiles of the aroma-dense muscadine grape, which is essential for future genetic or biotechnological improvements to attain a cultivar with the desired flavor. |
format | Online Article Text |
id | pubmed-8572961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85729612021-11-09 Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis Deng, Honghong He, Runmei Long, Meicun Li, Yanmei Zheng, Yuanyuan Lin, Lijin Liang, Dong Zhang, Xiaoai Liao, Ming'an Lv, Xiulan Deng, Qunxian Xia, Hui Front Plant Sci Plant Science Fruit aromas are composed of a complex mixture of volatile organic compounds, which are essential attributes associated with the overall flavor and consumer preference. Muscadine grape (MG; Vitis rotundifolia Michx.) is an aroma-dense fruit crop. However, there is very scarce information on its volatile profiles. In this study, the volatile constituents of five newly introduced MG cultivars, including Alachua, Carlos, Fry, Granny Val, and Noble, were profiled using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC/MS) combined with multivariate statistical analysis. A total of 44 compounds, including esters, aldehydes, alcohols, fatty acids, terpenes, ketones, and furan, were identified and relatively quantified. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) evidently discriminated against the five MG cultivars based on their volatile profiles. The specific volatiles that contributed the most to this discrimination were highlighted. Geraniol and cinnamyl alcohol were demonstrated to be essential for characterizing the Alachua MG cultivar, whereas ethyl trans-2-butenoate and propyl acetate were shown to be important compounds to characterize the Noble MG cultivar. The results further showed that 2-Ethyl-1-hexanol, (Z)-3-hexenal, and (E)-2-hexenol were closely related to Carlos, Fry, and Granny Val cultivars, respectively. This investigation is the first in-depth exploration of the volatile profiles of the aroma-dense muscadine grape, which is essential for future genetic or biotechnological improvements to attain a cultivar with the desired flavor. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8572961/ /pubmed/34759938 http://dx.doi.org/10.3389/fpls.2021.728891 Text en Copyright © 2021 Deng, He, Long, Li, Zheng, Lin, Liang, Zhang, Liao, Lv, Deng and Xia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Deng, Honghong He, Runmei Long, Meicun Li, Yanmei Zheng, Yuanyuan Lin, Lijin Liang, Dong Zhang, Xiaoai Liao, Ming'an Lv, Xiulan Deng, Qunxian Xia, Hui Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title | Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title_full | Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title_fullStr | Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title_full_unstemmed | Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title_short | Comparison of the Fruit Volatile Profiles of Five Muscadine Grape Cultivars (Vitis rotundifolia Michx.) Using HS-SPME-GC/MS Combined With Multivariate Statistical Analysis |
title_sort | comparison of the fruit volatile profiles of five muscadine grape cultivars (vitis rotundifolia michx.) using hs-spme-gc/ms combined with multivariate statistical analysis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572961/ https://www.ncbi.nlm.nih.gov/pubmed/34759938 http://dx.doi.org/10.3389/fpls.2021.728891 |
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