Cargando…

Structure-Based Primer Design Minimizes the Risk of PCR Failure Caused by SARS-CoV-2 Mutations

The coronavirus disease 2019 (COVID-19) has caused and is still causing tremendous damage to the global economy and human health. Qualitative reverse transcription-PCR (RT-qPCR) is the golden standard for COVID-19 test. However, the SARS-CoV-2 variants may not only make vaccine less effective but al...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dong, Hongjie, Wang, Shuai, Zhang, Junmei, Zhang, Kundi, Zhang, Fengyu, Wang, Hongwei, Xie, Shiling, Hu, Wei, Gu, Lichuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cellular and Infection Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573093/ https://www.ncbi.nlm.nih.gov/pubmed/34760717 http://dx.doi.org/10.3389/fcimb.2021.741147

Ejemplares similares

Wide Application of Minimally Processed Saliva on Multiple RT-qPCR Kits for SARS-CoV-2 Detection in Indonesia
por: Mahendra, Caroline, et al.
Publicado: (2021)

Antibiotic resistance and pathogenicity assessment of various Gardnerella sp. strains in local China
por: Zhang, Kundi, et al.
Publicado: (2022)

DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm
por: Liu, Nan, et al.
Publicado: (2021)

The Impact of Accumulated Mutations in SARS-CoV-2 Variants on the qPCR Detection Efficiency
por: Cao, Liu, et al.
Publicado: (2022)

Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method
por: Bohorquez, Jose Alejandro, et al.
Publicado: (2023)

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR
por: Wang, Di, et al.
Publicado: (2022)

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling
por: Heidrich, Vitor, et al.
Publicado: (2022)

Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening of Plasmodium falciparum
por: Hou, Nan, et al.
Publicado: (2021)

Establishment and Characterization of an Immortalized Porcine Oral Mucosal Epithelial Cell Line as a Cytopathogenic Model for Porcine Circovirus 2 Infection
por: Cui, Hongjie, et al.
Publicado: (2019)

Clinical evaluation of bacterial DNA using an improved droplet digital PCR for spontaneous bacterial peritonitis diagnosis
por: Wu, Hao-Xin, et al.
Publicado: (2022)

Evaluation of an identification method for the SARS-CoV-2 Delta variant based on the amplification-refractory mutation system
por: Zhang, Qin, et al.
Publicado: (2023)

Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR
por: He, Wenyin, et al.
Publicado: (2023)

Rapid Detection and Differentiating of the Predominant Salmonella Serovars in Chicken Farm by TaqMan Multiplex Real-Time PCR Assay
por: Xin, Suhua, et al.
Publicado: (2021)

Gut microbiota signature as predictors of adverse outcomes after acute ischemic stroke in patients with hyperlipidemia
por: Chen, Jiaxin, et al.
Publicado: (2022)

Utility of Multi-target Nested PCR and ELISPOT Assays for the Detection of Paucibacillary Leprosy: A Possible Conclusion of Clinical Laboratory Misdiagnosis
por: Jiang, Haiqin, et al.
Publicado: (2022)

Classification of subtypes and identification of dysregulated genes in sepsis
por: Tong, Ran, et al.
Publicado: (2023)

Application of Quantitative PCR in the Diagnosis and Evaluating Treatment Efficacy of Leishmaniasis
por: Wu, Yun, et al.
Publicado: (2020)

Broad-spectrum humanized monoclonal neutralizing antibody against SARS-CoV-2 variants, including the Omicron variant
por: Wen, Kun, et al.
Publicado: (2023)

Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment
por: Zhao, Shuo, et al.
Publicado: (2021)

A LysR Transcriptional Regulator Manipulates Macrophage Autophagy Flux During Brucella Infection
por: Zhang, Lu, et al.
Publicado: (2022)

In-Silico Detection of Oral Prokaryotic Species With Highly Similar 16S rRNA Sequence Segments Using Different Primer Pairs
por: Regueira-Iglesias, Alba, et al.
Publicado: (2022)

Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform
por: Li, Zhenpeng, et al.
Publicado: (2021)

SARS-CoV-2 RdRp uses NDPs as a substrate and is able to incorporate NHC into RNA from diphosphate form molnupiravir
por: Wang, Maofeng, et al.
Publicado: (2023)

Rapid and Accurate Identification of SARS-CoV-2 Variants Using Real Time PCR Assays
por: Borillo, Gwynngelle A., et al.
Publicado: (2022)

Underdevelopment of gut microbiota in failure to thrive infants of up to 12 months of age
por: Zhang, Mei, et al.
Publicado: (2022)

Whole Genome Sequencing Identifies Novel Mutations Associated With Bedaquiline Resistance in Mycobacterium tuberculosis
por: Guo, Qinglong, et al.
Publicado: (2022)

Microbiome–transcriptome analysis reveals that dietary supplementation with macleaya cordata extract alters multiple immune pathways with minimal impact on microbial structure
por: Wu, Jian, et al.
Publicado: (2023)

Development of a panel of three multiplex allele-specific qRT-PCR assays for quick differentiation of recombinant variants and Omicron subvariants of SARS-CoV-2
por: Li, Jianguo, et al.
Publicado: (2022)

Changes in the gut microbiota structure and function in rats with doxorubicin-induced heart failure
por: Fan, Yawen, et al.
Publicado: (2023)

A droplet digital PCR assay for detection and quantification of Verticillium nonalfalfae and V. albo-atrum
por: Wang, Di, et al.
Publicado: (2023)

RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
por: Zhang, Fengyu, et al.
Publicado: (2023)

Comorbidities and Age Are Associated With Persistent COVID-19 PCR Positivity
por: Aldhaeefi, Mohammed, et al.
Publicado: (2021)

RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact
por: Vega-Magaña, Natali, et al.
Publicado: (2021)

Cotton-Tipped Plastic Swabs for SARS-CoV-2 RT-qPCR Diagnosis to Prevent Supply Shortages
por: Freire-Paspuel, Byron, et al.
Publicado: (2020)

Isolation of SARS-CoV-2 in Viral Cell Culture in Immunocompromised Patients With Persistently Positive RT-PCR Results
por: Sung, Abby, et al.
Publicado: (2022)

Influence of Fluoride-Resistant Streptococcus mutans Within Antagonistic Dual-Species Biofilms Under Fluoride In Vitro
por: Zhang, Keke, et al.
Publicado: (2022)

Characteristics of the fecal microbiome and metabolome in older patients with heart failure and sarcopenia
por: Peng, Jieting, et al.
Publicado: (2023)

Evaluating the effect of SARS-CoV-2 spike mutations with a linear doubly robust learner
por: Wang, Xin, et al.
Publicado: (2023)

Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions
por: Hu, Xiao-Mei, et al.
Publicado: (2019)

A modification of nested PCR method for detection of Enterocytozoon hepatopenaei (EHP) in giant freshwater prawn Macrobrachium rosenbergii
por: Wang, Yuan, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_cj76hhdc09v42fulo4rcj5fghp