Cargando…
A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis
Circular RNAs (circRNAs) are a novel class of widespread noncoding RNAs that regulate gene expression in mammals. Recent studies demonstrate that functional peptides can be encoded by short open reading frames in noncoding RNAs, including circRNAs. However, the role of circRNAs in various physiologi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573159/ https://www.ncbi.nlm.nih.gov/pubmed/34534547 http://dx.doi.org/10.1016/j.jbc.2021.101182 |
_version_ | 1784595362395193344 |
---|---|
author | Li, Fang Cai, Yuhan Deng, Sihan Yang, Lin Liu, Na Chang, Xiaohan Jing, Lankai Zhou, Yifeng Li, Hua |
author_facet | Li, Fang Cai, Yuhan Deng, Sihan Yang, Lin Liu, Na Chang, Xiaohan Jing, Lankai Zhou, Yifeng Li, Hua |
author_sort | Li, Fang |
collection | PubMed |
description | Circular RNAs (circRNAs) are a novel class of widespread noncoding RNAs that regulate gene expression in mammals. Recent studies demonstrate that functional peptides can be encoded by short open reading frames in noncoding RNAs, including circRNAs. However, the role of circRNAs in various physiological and pathological states, such as cancer, is not well understood. In this study, through deep RNA sequencing on human endometrial cancer (EC) samples and their paired adjacent normal tissues, we uncovered that the circRNA hsa-circ-0000437 is significantly reduced in EC compared with matched paracancerous tissue. The hsa-circ-0000437 contains a short open reading frame encoding a functional peptide termed CORO1C-47aa. Overexpression of CORO1C-47aa is capable of inhibiting angiogenesis at the initiation stage by suppressing endothelial cell proliferation, migration, and differentiation through competition with transcription factor TACC3 to bind to ARNT and suppress VEGF. CORO1C-47aa directly bound to ARNT through the PAS-B domain, and blocking the association between ARNT and TACC3, which led to reduced expression of VEGF, ultimately lead to reduced angiogenesis. The antitumor effects of CORO1C-47aa on EC progression suggest that CORO1C-47aa has potential value in anticarcinoma therapies and warrants further investigation. |
format | Online Article Text |
id | pubmed-8573159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-85731592021-11-10 A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis Li, Fang Cai, Yuhan Deng, Sihan Yang, Lin Liu, Na Chang, Xiaohan Jing, Lankai Zhou, Yifeng Li, Hua J Biol Chem Research Article Circular RNAs (circRNAs) are a novel class of widespread noncoding RNAs that regulate gene expression in mammals. Recent studies demonstrate that functional peptides can be encoded by short open reading frames in noncoding RNAs, including circRNAs. However, the role of circRNAs in various physiological and pathological states, such as cancer, is not well understood. In this study, through deep RNA sequencing on human endometrial cancer (EC) samples and their paired adjacent normal tissues, we uncovered that the circRNA hsa-circ-0000437 is significantly reduced in EC compared with matched paracancerous tissue. The hsa-circ-0000437 contains a short open reading frame encoding a functional peptide termed CORO1C-47aa. Overexpression of CORO1C-47aa is capable of inhibiting angiogenesis at the initiation stage by suppressing endothelial cell proliferation, migration, and differentiation through competition with transcription factor TACC3 to bind to ARNT and suppress VEGF. CORO1C-47aa directly bound to ARNT through the PAS-B domain, and blocking the association between ARNT and TACC3, which led to reduced expression of VEGF, ultimately lead to reduced angiogenesis. The antitumor effects of CORO1C-47aa on EC progression suggest that CORO1C-47aa has potential value in anticarcinoma therapies and warrants further investigation. American Society for Biochemistry and Molecular Biology 2021-09-14 /pmc/articles/PMC8573159/ /pubmed/34534547 http://dx.doi.org/10.1016/j.jbc.2021.101182 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Li, Fang Cai, Yuhan Deng, Sihan Yang, Lin Liu, Na Chang, Xiaohan Jing, Lankai Zhou, Yifeng Li, Hua A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title | A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title_full | A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title_fullStr | A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title_full_unstemmed | A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title_short | A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
title_sort | peptide coro1c-47aa encoded by the circular noncoding rna circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573159/ https://www.ncbi.nlm.nih.gov/pubmed/34534547 http://dx.doi.org/10.1016/j.jbc.2021.101182 |
work_keys_str_mv | AT lifang apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT caiyuhan apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT dengsihan apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT yanglin apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT liuna apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT changxiaohan apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT jinglankai apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT zhouyifeng apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT lihua apeptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT lifang peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT caiyuhan peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT dengsihan peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT yanglin peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT liuna peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT changxiaohan peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT jinglankai peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT zhouyifeng peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis AT lihua peptidecoro1c47aaencodedbythecircularnoncodingrnacirc0000437functionsasanegativeregulatorinendometriumtumorangiogenesis |