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Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures
ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573202/ https://www.ncbi.nlm.nih.gov/pubmed/34760879 http://dx.doi.org/10.3389/fbioe.2021.754250 |
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author | Jang, Yu-Sin Seong, Hyeon Jeong Kwon, Seong Woo Lee, Yong-Suk Im, Jung Ae Lee, Haeng Lim Yoon, Ye Rin Lee, Sang Yup |
author_facet | Jang, Yu-Sin Seong, Hyeon Jeong Kwon, Seong Woo Lee, Yong-Suk Im, Jung Ae Lee, Haeng Lim Yoon, Ye Rin Lee, Sang Yup |
author_sort | Jang, Yu-Sin |
collection | PubMed |
description | ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the atpG gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth compared with the parent ATCC 824. However, the pH value in 824-2866KD cultures at the late stage of the solventogenic phase was abnormally high (pH 6.12), compared with that obtained routinely in the culture of ATCC 824 (pH 5.74). This phenomenon was also observed in batch cultures of another C. acetobutylicum, BEKW-2866KD, an atpG-knockdown and pta-buk double-knockout mutant. The findings reported in this study suggested that ATPase is relatively minor than acid-forming pathway in ATP metabolism in C. acetobutylicum. |
format | Online Article Text |
id | pubmed-8573202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85732022021-11-09 Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures Jang, Yu-Sin Seong, Hyeon Jeong Kwon, Seong Woo Lee, Yong-Suk Im, Jung Ae Lee, Haeng Lim Yoon, Ye Rin Lee, Sang Yup Front Bioeng Biotechnol Bioengineering and Biotechnology ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the atpG gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth compared with the parent ATCC 824. However, the pH value in 824-2866KD cultures at the late stage of the solventogenic phase was abnormally high (pH 6.12), compared with that obtained routinely in the culture of ATCC 824 (pH 5.74). This phenomenon was also observed in batch cultures of another C. acetobutylicum, BEKW-2866KD, an atpG-knockdown and pta-buk double-knockout mutant. The findings reported in this study suggested that ATPase is relatively minor than acid-forming pathway in ATP metabolism in C. acetobutylicum. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8573202/ /pubmed/34760879 http://dx.doi.org/10.3389/fbioe.2021.754250 Text en Copyright © 2021 Jang, Seong, Kwon, Lee, Im, Lee, Yoon and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Jang, Yu-Sin Seong, Hyeon Jeong Kwon, Seong Woo Lee, Yong-Suk Im, Jung Ae Lee, Haeng Lim Yoon, Ye Rin Lee, Sang Yup Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title |
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title_full |
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title_fullStr |
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title_full_unstemmed |
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title_short |
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures |
title_sort | clostridium acetobutylicum atpg-knockdown mutants increase extracellular ph in batch cultures |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573202/ https://www.ncbi.nlm.nih.gov/pubmed/34760879 http://dx.doi.org/10.3389/fbioe.2021.754250 |
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