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Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1

The cultivation of dolichos bean [Lablab purpureus (L.) Sweet] has been severely affected by dolichos yellow mosaic virus (DoYMV, Begomovirus) transmitted by whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). We tested the transovarial transmission of DoYMV in next-generation B. tabaci by PCR, real-...

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Autores principales: Ghosh, Amalendu, Roy, Buddhadeb, Nekkanti, Aarthi, Das, Amrita, Dhar, Shri, Mukherjee, Sunil Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573353/
https://www.ncbi.nlm.nih.gov/pubmed/34759905
http://dx.doi.org/10.3389/fmicb.2021.755155
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author Ghosh, Amalendu
Roy, Buddhadeb
Nekkanti, Aarthi
Das, Amrita
Dhar, Shri
Mukherjee, Sunil Kumar
author_facet Ghosh, Amalendu
Roy, Buddhadeb
Nekkanti, Aarthi
Das, Amrita
Dhar, Shri
Mukherjee, Sunil Kumar
author_sort Ghosh, Amalendu
collection PubMed
description The cultivation of dolichos bean [Lablab purpureus (L.) Sweet] has been severely affected by dolichos yellow mosaic virus (DoYMV, Begomovirus) transmitted by whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). We tested the transovarial transmission of DoYMV in next-generation B. tabaci by PCR, real-time PCR, Southern blot hybridization, and biological transmission. The eggs, laid by DoYMV-exposed B. tabaci, carry the virus in a unique pattern. Only the eggs laid in between 3 and 6 days post virus acquisition by a parent B. tabaci were DoYMV positive. When tested individually in real-time PCR, around 31–53% of the eggs carried the virus. The presence of DoYMV in ovaries and F1 eggs was further substantiated by the hybridization of a Cy3-conjugated nucleic acid probe complementary to the viral strand of DoYMV. Viral DNA was also detected in F1 adults and F2 eggs. B. tabaci progenies carried not only the DoYMV DNA but were also infective. The F1 adults transmitted DoYMV to all tested plants and produced strong yellow mosaic symptoms. An increase in viral copies from egg to nymphal stage indicated propagation of DoYMV in B. tabaci. However, the increase was for a short period and decreased thereafter. The present study provides the first evidence of transovarial transmission and propagation of a bipartite begomovirus in its vector, B. tabaci Asia II 1. The transovarial transmission and replication of DoYMV in B. tabaci have great epidemiological relevance as B. tabaci can serve as a major host of the virus to bridge the gap between the cropping seasons.
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spelling pubmed-85733532021-11-09 Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1 Ghosh, Amalendu Roy, Buddhadeb Nekkanti, Aarthi Das, Amrita Dhar, Shri Mukherjee, Sunil Kumar Front Microbiol Microbiology The cultivation of dolichos bean [Lablab purpureus (L.) Sweet] has been severely affected by dolichos yellow mosaic virus (DoYMV, Begomovirus) transmitted by whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). We tested the transovarial transmission of DoYMV in next-generation B. tabaci by PCR, real-time PCR, Southern blot hybridization, and biological transmission. The eggs, laid by DoYMV-exposed B. tabaci, carry the virus in a unique pattern. Only the eggs laid in between 3 and 6 days post virus acquisition by a parent B. tabaci were DoYMV positive. When tested individually in real-time PCR, around 31–53% of the eggs carried the virus. The presence of DoYMV in ovaries and F1 eggs was further substantiated by the hybridization of a Cy3-conjugated nucleic acid probe complementary to the viral strand of DoYMV. Viral DNA was also detected in F1 adults and F2 eggs. B. tabaci progenies carried not only the DoYMV DNA but were also infective. The F1 adults transmitted DoYMV to all tested plants and produced strong yellow mosaic symptoms. An increase in viral copies from egg to nymphal stage indicated propagation of DoYMV in B. tabaci. However, the increase was for a short period and decreased thereafter. The present study provides the first evidence of transovarial transmission and propagation of a bipartite begomovirus in its vector, B. tabaci Asia II 1. The transovarial transmission and replication of DoYMV in B. tabaci have great epidemiological relevance as B. tabaci can serve as a major host of the virus to bridge the gap between the cropping seasons. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8573353/ /pubmed/34759905 http://dx.doi.org/10.3389/fmicb.2021.755155 Text en Copyright © 2021 Ghosh, Roy, Nekkanti, Das, Dhar and Mukherjee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ghosh, Amalendu
Roy, Buddhadeb
Nekkanti, Aarthi
Das, Amrita
Dhar, Shri
Mukherjee, Sunil Kumar
Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title_full Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title_fullStr Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title_full_unstemmed Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title_short Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
title_sort transovarial transmission of dolichos yellow mosaic virus by its vector, bemisia tabaci asia ii 1
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573353/
https://www.ncbi.nlm.nih.gov/pubmed/34759905
http://dx.doi.org/10.3389/fmicb.2021.755155
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