Cargando…
A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibili...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573415/ https://www.ncbi.nlm.nih.gov/pubmed/34759958 http://dx.doi.org/10.3389/fgene.2021.744165 |
_version_ | 1784595417768394752 |
---|---|
author | Gourain, Victor Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël |
author_facet | Gourain, Victor Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël |
author_sort | Gourain, Victor |
collection | PubMed |
description | Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibility encoded by the Cdx2 homeobox gene that plays important functions during embryonic development and in adult diseases. By integrating RNAseq data in mouse embryogenesis, we unveiled a core set of common genes whose expression is responsive to the CDX2 homeoprotein during trophectoderm formation, posterior body elongation and intestinal specification. ChIPseq data analysis also identified a set of common chromosomal regions targeted by CDX2 at these three developmental steps. The transcriptional core set of genes was then validated with transgenic mouse models of loss or gain of function of Cdx2. Finally, based on human cancer data, we highlight the relevance of these results by displaying a significant number of human orthologous genes to the core set of mouse CDX2-responsive genes exhibiting an altered expression along with CDX2 in human malignancies. |
format | Online Article Text |
id | pubmed-8573415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85734152021-11-09 A Core Response to the CDX2 Homeoprotein During Development and in Pathologies Gourain, Victor Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël Front Genet Genetics Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibility encoded by the Cdx2 homeobox gene that plays important functions during embryonic development and in adult diseases. By integrating RNAseq data in mouse embryogenesis, we unveiled a core set of common genes whose expression is responsive to the CDX2 homeoprotein during trophectoderm formation, posterior body elongation and intestinal specification. ChIPseq data analysis also identified a set of common chromosomal regions targeted by CDX2 at these three developmental steps. The transcriptional core set of genes was then validated with transgenic mouse models of loss or gain of function of Cdx2. Finally, based on human cancer data, we highlight the relevance of these results by displaying a significant number of human orthologous genes to the core set of mouse CDX2-responsive genes exhibiting an altered expression along with CDX2 in human malignancies. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8573415/ /pubmed/34759958 http://dx.doi.org/10.3389/fgene.2021.744165 Text en Copyright © 2021 Gourain, Duluc, Domon-Dell and Freund. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Gourain, Victor Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title | A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title_full | A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title_fullStr | A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title_full_unstemmed | A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title_short | A Core Response to the CDX2 Homeoprotein During Development and in Pathologies |
title_sort | core response to the cdx2 homeoprotein during development and in pathologies |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573415/ https://www.ncbi.nlm.nih.gov/pubmed/34759958 http://dx.doi.org/10.3389/fgene.2021.744165 |
work_keys_str_mv | AT gourainvictor acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT dulucisabelle acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT domondellclaire acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT freundjeannoel acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT gourainvictor coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT dulucisabelle coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT domondellclaire coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies AT freundjeannoel coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies |