Cargando…

A Core Response to the CDX2 Homeoprotein During Development and in Pathologies

Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibili...

Descripción completa

Detalles Bibliográficos
Autores principales: Gourain, Victor, Duluc, Isabelle, Domon-Dell, Claire, Freund, Jean-Noël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573415/
https://www.ncbi.nlm.nih.gov/pubmed/34759958
http://dx.doi.org/10.3389/fgene.2021.744165
_version_ 1784595417768394752
author Gourain, Victor
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
author_facet Gourain, Victor
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
author_sort Gourain, Victor
collection PubMed
description Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibility encoded by the Cdx2 homeobox gene that plays important functions during embryonic development and in adult diseases. By integrating RNAseq data in mouse embryogenesis, we unveiled a core set of common genes whose expression is responsive to the CDX2 homeoprotein during trophectoderm formation, posterior body elongation and intestinal specification. ChIPseq data analysis also identified a set of common chromosomal regions targeted by CDX2 at these three developmental steps. The transcriptional core set of genes was then validated with transgenic mouse models of loss or gain of function of Cdx2. Finally, based on human cancer data, we highlight the relevance of these results by displaying a significant number of human orthologous genes to the core set of mouse CDX2-responsive genes exhibiting an altered expression along with CDX2 in human malignancies.
format Online
Article
Text
id pubmed-8573415
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85734152021-11-09 A Core Response to the CDX2 Homeoprotein During Development and in Pathologies Gourain, Victor Duluc, Isabelle Domon-Dell, Claire Freund, Jean-Noël Front Genet Genetics Whether a gene involved in distinct tissue or cell functions exerts a core of common molecular activities is a relevant topic in evolutionary, developmental, and pathological perspectives. Here, we addressed this question by focusing on the transcription factor and regulator of chromatin accessibility encoded by the Cdx2 homeobox gene that plays important functions during embryonic development and in adult diseases. By integrating RNAseq data in mouse embryogenesis, we unveiled a core set of common genes whose expression is responsive to the CDX2 homeoprotein during trophectoderm formation, posterior body elongation and intestinal specification. ChIPseq data analysis also identified a set of common chromosomal regions targeted by CDX2 at these three developmental steps. The transcriptional core set of genes was then validated with transgenic mouse models of loss or gain of function of Cdx2. Finally, based on human cancer data, we highlight the relevance of these results by displaying a significant number of human orthologous genes to the core set of mouse CDX2-responsive genes exhibiting an altered expression along with CDX2 in human malignancies. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8573415/ /pubmed/34759958 http://dx.doi.org/10.3389/fgene.2021.744165 Text en Copyright © 2021 Gourain, Duluc, Domon-Dell and Freund. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Gourain, Victor
Duluc, Isabelle
Domon-Dell, Claire
Freund, Jean-Noël
A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title_full A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title_fullStr A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title_full_unstemmed A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title_short A Core Response to the CDX2 Homeoprotein During Development and in Pathologies
title_sort core response to the cdx2 homeoprotein during development and in pathologies
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573415/
https://www.ncbi.nlm.nih.gov/pubmed/34759958
http://dx.doi.org/10.3389/fgene.2021.744165
work_keys_str_mv AT gourainvictor acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT dulucisabelle acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT domondellclaire acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT freundjeannoel acoreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT gourainvictor coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT dulucisabelle coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT domondellclaire coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies
AT freundjeannoel coreresponsetothecdx2homeoproteinduringdevelopmentandinpathologies