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Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors

BACKGROUND: Cellular immune monitoring is becoming more critical in the clinic, but its application has not yet become sufficiently widespread. One reason may be the different reference intervals among clinical laboratories due to several factors. Percentage and number of lymphocyte subsets are stan...

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Autores principales: Xu, Kangli, Miao, Linzi, Chen, Weiye, Wu, Hui, Gong, Yan, Tu, Xiaoxin, Guo, Wei, Pan, Baishen, Qu, Chenxue, Wu, Xinzhong, Wang, Beili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573445/
https://www.ncbi.nlm.nih.gov/pubmed/34805357
http://dx.doi.org/10.21037/atm-21-4031
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author Xu, Kangli
Miao, Linzi
Chen, Weiye
Wu, Hui
Gong, Yan
Tu, Xiaoxin
Guo, Wei
Pan, Baishen
Qu, Chenxue
Wu, Xinzhong
Wang, Beili
author_facet Xu, Kangli
Miao, Linzi
Chen, Weiye
Wu, Hui
Gong, Yan
Tu, Xiaoxin
Guo, Wei
Pan, Baishen
Qu, Chenxue
Wu, Xinzhong
Wang, Beili
author_sort Xu, Kangli
collection PubMed
description BACKGROUND: Cellular immune monitoring is becoming more critical in the clinic, but its application has not yet become sufficiently widespread. One reason may be the different reference intervals among clinical laboratories due to several factors. Percentage and number of lymphocyte subsets are standard indicators of cellular immune detection. The present study aimed to establish standardized reference intervals of lymphocyte subsets in the healthy Chinese Han adult population and examine such influencing factors as age, gender, region, and measurement instruments. METHODS: A total of 496 healthy Chinese Han people aged 18–59 years from 3 China Mainland regions (north, east, and south) were enrolled. The sample of each center was simultaneously examined by three flow cytometers (FACSCanto(TM)II, FACSLyric(TM), and FACSCalibur(TM)). A single-platform flow cytometry-based absolute count technique was used to quantify the percentage and number of each lymphocyte subset. The flow cytometry results were analyzed by variance analysis and Z test to determine the influence of age, gender, and instruments on lymphocyte subsets. RESULTS: Multi-center, age-specific, and gender-specific reference intervals of healthy Chinese Han adults’ lymphocyte subsets were established. There was no statistical difference in the results from the three flow cytometers. Gender affected the results of CD4(+) (%) and the absolute count of CD3(−)CD16(+)CD56(+), where CD4(+) (%) was higher in women, and the absolute count of CD3(−)CD16(+)CD56(+) was higher in men. Age mainly affected the CD4(+)/CD8(+) ratio, which was statistically higher in groups aged over 40 years; the percentage and number of CD3(−)CD19(+) were more elevated in age groups below 30 years; however, the difference was not statistically significant. CONCLUSIONS: This study established the reference intervals of lymphocyte subsets for healthy Chinese Han adult populations under the standardized methods. This study was the first nationwide study in China to use a flow cytometry-based single-platform method to establish the reference intervals of lymphocyte subsets of the healthy Chinese Han adult population. Gender and age were shown to influence the results of lymphocyte subsets.
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spelling pubmed-85734452021-11-18 Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors Xu, Kangli Miao, Linzi Chen, Weiye Wu, Hui Gong, Yan Tu, Xiaoxin Guo, Wei Pan, Baishen Qu, Chenxue Wu, Xinzhong Wang, Beili Ann Transl Med Original Article BACKGROUND: Cellular immune monitoring is becoming more critical in the clinic, but its application has not yet become sufficiently widespread. One reason may be the different reference intervals among clinical laboratories due to several factors. Percentage and number of lymphocyte subsets are standard indicators of cellular immune detection. The present study aimed to establish standardized reference intervals of lymphocyte subsets in the healthy Chinese Han adult population and examine such influencing factors as age, gender, region, and measurement instruments. METHODS: A total of 496 healthy Chinese Han people aged 18–59 years from 3 China Mainland regions (north, east, and south) were enrolled. The sample of each center was simultaneously examined by three flow cytometers (FACSCanto(TM)II, FACSLyric(TM), and FACSCalibur(TM)). A single-platform flow cytometry-based absolute count technique was used to quantify the percentage and number of each lymphocyte subset. The flow cytometry results were analyzed by variance analysis and Z test to determine the influence of age, gender, and instruments on lymphocyte subsets. RESULTS: Multi-center, age-specific, and gender-specific reference intervals of healthy Chinese Han adults’ lymphocyte subsets were established. There was no statistical difference in the results from the three flow cytometers. Gender affected the results of CD4(+) (%) and the absolute count of CD3(−)CD16(+)CD56(+), where CD4(+) (%) was higher in women, and the absolute count of CD3(−)CD16(+)CD56(+) was higher in men. Age mainly affected the CD4(+)/CD8(+) ratio, which was statistically higher in groups aged over 40 years; the percentage and number of CD3(−)CD19(+) were more elevated in age groups below 30 years; however, the difference was not statistically significant. CONCLUSIONS: This study established the reference intervals of lymphocyte subsets for healthy Chinese Han adult populations under the standardized methods. This study was the first nationwide study in China to use a flow cytometry-based single-platform method to establish the reference intervals of lymphocyte subsets of the healthy Chinese Han adult population. Gender and age were shown to influence the results of lymphocyte subsets. AME Publishing Company 2021-10 /pmc/articles/PMC8573445/ /pubmed/34805357 http://dx.doi.org/10.21037/atm-21-4031 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Xu, Kangli
Miao, Linzi
Chen, Weiye
Wu, Hui
Gong, Yan
Tu, Xiaoxin
Guo, Wei
Pan, Baishen
Qu, Chenxue
Wu, Xinzhong
Wang, Beili
Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title_full Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title_fullStr Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title_full_unstemmed Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title_short Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors
title_sort establishment of the reference intervals of lymphocyte subsets for healthy chinese han adults and its influencing factors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573445/
https://www.ncbi.nlm.nih.gov/pubmed/34805357
http://dx.doi.org/10.21037/atm-21-4031
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