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Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma

Lisianthus (Eustoma grandiflorum) is an important floricultural crop cultivated worldwide. Despite its commercial importance, few DNA markers are available for molecular genetic research. In this study, we constructed a genetic linkage map and to detect quantitative trait loci (QTLs) for important a...

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Autores principales: Kawakatsu, Kyoko, Yagi, Masafumi, Harada, Taro, Yamaguchi, Hiroyasu, Itoh, Takeshi, Kumagai, Masahiko, Itoh, Ryutaro, Numa, Hisataka, Katayose, Yuichi, Kanamori, Hiroyuki, Kurita, Kanako, Fukuta, Naoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573551/
https://www.ncbi.nlm.nih.gov/pubmed/34776741
http://dx.doi.org/10.1270/jsbbs.20100
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author Kawakatsu, Kyoko
Yagi, Masafumi
Harada, Taro
Yamaguchi, Hiroyasu
Itoh, Takeshi
Kumagai, Masahiko
Itoh, Ryutaro
Numa, Hisataka
Katayose, Yuichi
Kanamori, Hiroyuki
Kurita, Kanako
Fukuta, Naoko
author_facet Kawakatsu, Kyoko
Yagi, Masafumi
Harada, Taro
Yamaguchi, Hiroyasu
Itoh, Takeshi
Kumagai, Masahiko
Itoh, Ryutaro
Numa, Hisataka
Katayose, Yuichi
Kanamori, Hiroyuki
Kurita, Kanako
Fukuta, Naoko
author_sort Kawakatsu, Kyoko
collection PubMed
description Lisianthus (Eustoma grandiflorum) is an important floricultural crop cultivated worldwide. Despite its commercial importance, few DNA markers are available for molecular genetic research. In this study, we constructed a genetic linkage map and to detect quantitative trait loci (QTLs) for important agronomic traits of lisianthus. To develop simple sequence repeat (SSR) markers, we used 454-pyrosequencing technology to obtain genomic shotgun sequences and subsequently identified 8263 putative SSRs. A total of 3990 primer pairs were designed in silico and 1189 unique primer pairs were extracted through a BLAST search. Amplification was successful for more than 1000 primer pairs, and ultimately 278 SSR markers exhibited polymorphism between the two lisianthus accessions evaluated. Based on these markers, a genetic linkage map was constructed using a breeding population derived from crosses between the two accessions, for which flowering time differed (>140 days when grown under 20°C). We detected one QTL associated with flowering time (phenotypic variance, 27%; LOD value, 3.7). The SSR marker located at this QTL may account for variation in flowering time among accessions (i.e., three accessions whose nodes of the first flower were over 30 had late-flowering alleles of this QTL).
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spelling pubmed-85735512021-11-12 Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma Kawakatsu, Kyoko Yagi, Masafumi Harada, Taro Yamaguchi, Hiroyasu Itoh, Takeshi Kumagai, Masahiko Itoh, Ryutaro Numa, Hisataka Katayose, Yuichi Kanamori, Hiroyuki Kurita, Kanako Fukuta, Naoko Breed Sci Research Paper Lisianthus (Eustoma grandiflorum) is an important floricultural crop cultivated worldwide. Despite its commercial importance, few DNA markers are available for molecular genetic research. In this study, we constructed a genetic linkage map and to detect quantitative trait loci (QTLs) for important agronomic traits of lisianthus. To develop simple sequence repeat (SSR) markers, we used 454-pyrosequencing technology to obtain genomic shotgun sequences and subsequently identified 8263 putative SSRs. A total of 3990 primer pairs were designed in silico and 1189 unique primer pairs were extracted through a BLAST search. Amplification was successful for more than 1000 primer pairs, and ultimately 278 SSR markers exhibited polymorphism between the two lisianthus accessions evaluated. Based on these markers, a genetic linkage map was constructed using a breeding population derived from crosses between the two accessions, for which flowering time differed (>140 days when grown under 20°C). We detected one QTL associated with flowering time (phenotypic variance, 27%; LOD value, 3.7). The SSR marker located at this QTL may account for variation in flowering time among accessions (i.e., three accessions whose nodes of the first flower were over 30 had late-flowering alleles of this QTL). Japanese Society of Breeding 2021-06 2021-06-25 /pmc/articles/PMC8573551/ /pubmed/34776741 http://dx.doi.org/10.1270/jsbbs.20100 Text en Copyright © 2021 by JAPANESE SOCIETY OF BREEDING https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Kawakatsu, Kyoko
Yagi, Masafumi
Harada, Taro
Yamaguchi, Hiroyasu
Itoh, Takeshi
Kumagai, Masahiko
Itoh, Ryutaro
Numa, Hisataka
Katayose, Yuichi
Kanamori, Hiroyuki
Kurita, Kanako
Fukuta, Naoko
Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title_full Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title_fullStr Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title_full_unstemmed Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title_short Development of an SSR marker-based genetic linkage map and identification of a QTL associated with flowering time in Eustoma
title_sort development of an ssr marker-based genetic linkage map and identification of a qtl associated with flowering time in eustoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573551/
https://www.ncbi.nlm.nih.gov/pubmed/34776741
http://dx.doi.org/10.1270/jsbbs.20100
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