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Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes

The effectiveness of advanced technologies on eliminating antibiotic resistant bacteria (ARB) and resistance genes (ARGs) from wastewaters have been recently investigated. Solar photo-Fenton has been proven effective in combating ARB and ARGs from Municipal Wastewater Treatment Plant effluent (MWWTP...

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Autores principales: Vilela, Pâmela B., Mendonça Neto, Rondon P., Starling, Maria Clara V.M., da S. Martins, Alessandra, Pires, Giovanna F.F., Souza, Felipe A.R., Amorim, Camila C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573595/
https://www.ncbi.nlm.nih.gov/pubmed/34467925
http://dx.doi.org/10.1016/j.scitotenv.2021.149599
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author Vilela, Pâmela B.
Mendonça Neto, Rondon P.
Starling, Maria Clara V.M.
da S. Martins, Alessandra
Pires, Giovanna F.F.
Souza, Felipe A.R.
Amorim, Camila C.
author_facet Vilela, Pâmela B.
Mendonça Neto, Rondon P.
Starling, Maria Clara V.M.
da S. Martins, Alessandra
Pires, Giovanna F.F.
Souza, Felipe A.R.
Amorim, Camila C.
author_sort Vilela, Pâmela B.
collection PubMed
description The effectiveness of advanced technologies on eliminating antibiotic resistant bacteria (ARB) and resistance genes (ARGs) from wastewaters have been recently investigated. Solar photo-Fenton has been proven effective in combating ARB and ARGs from Municipal Wastewater Treatment Plant effluent (MWWTPE). However, most of these studies have relied solely on cultivable methods to assess ARB removal. This is the first study to investigate the effect of solar photo-Fenton upon ARB and ARGs in MWWTPE by high throughput metagenomic analysis (16S rDNA sequencing and Whole Genome Sequencing). Treatment efficiency upon priority pathogens and resistome profile were also investigated. Solar photo-Fenton (30 mg L(−1) of Fe(2+) intermittent additions and 50 mg L(−1) of H(2)O(2)) reached 76–86% removal of main phyla present in MWWTPE. An increase in Proteobacteria abundance was observed after solar photo-Fenton and controls in which H(2)O(2) was present as an oxidant (Fenton, H(2)O(2) only, solar/H(2)O(2)). Hence, tolerance mechanisms presented by this group should be further assessed. Solar photo-Fenton achieved complete removal of high priority Staphylococcus and Enterococcus, as well as Klebsiella pneumoniae and Pseudomonas aeruginosa. Substantial reduction of intrinsically multi-drug resistant bacteria was detected. Solar photo-Fenton removed nearly 60% of ARGs associated with sulfonamides, macrolides, and tetracyclines, and complete removal of ARGs related to β-lactams and fluoroquinolones. These results indicate the potential of using solar-enhanced photo-Fenton to limit the spread of antimicrobial resistance, especially in developing tropical countries.
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spelling pubmed-85735952021-12-20 Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes Vilela, Pâmela B. Mendonça Neto, Rondon P. Starling, Maria Clara V.M. da S. Martins, Alessandra Pires, Giovanna F.F. Souza, Felipe A.R. Amorim, Camila C. Sci Total Environ Article The effectiveness of advanced technologies on eliminating antibiotic resistant bacteria (ARB) and resistance genes (ARGs) from wastewaters have been recently investigated. Solar photo-Fenton has been proven effective in combating ARB and ARGs from Municipal Wastewater Treatment Plant effluent (MWWTPE). However, most of these studies have relied solely on cultivable methods to assess ARB removal. This is the first study to investigate the effect of solar photo-Fenton upon ARB and ARGs in MWWTPE by high throughput metagenomic analysis (16S rDNA sequencing and Whole Genome Sequencing). Treatment efficiency upon priority pathogens and resistome profile were also investigated. Solar photo-Fenton (30 mg L(−1) of Fe(2+) intermittent additions and 50 mg L(−1) of H(2)O(2)) reached 76–86% removal of main phyla present in MWWTPE. An increase in Proteobacteria abundance was observed after solar photo-Fenton and controls in which H(2)O(2) was present as an oxidant (Fenton, H(2)O(2) only, solar/H(2)O(2)). Hence, tolerance mechanisms presented by this group should be further assessed. Solar photo-Fenton achieved complete removal of high priority Staphylococcus and Enterococcus, as well as Klebsiella pneumoniae and Pseudomonas aeruginosa. Substantial reduction of intrinsically multi-drug resistant bacteria was detected. Solar photo-Fenton removed nearly 60% of ARGs associated with sulfonamides, macrolides, and tetracyclines, and complete removal of ARGs related to β-lactams and fluoroquinolones. These results indicate the potential of using solar-enhanced photo-Fenton to limit the spread of antimicrobial resistance, especially in developing tropical countries. Elsevier 2021-12-20 /pmc/articles/PMC8573595/ /pubmed/34467925 http://dx.doi.org/10.1016/j.scitotenv.2021.149599 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vilela, Pâmela B.
Mendonça Neto, Rondon P.
Starling, Maria Clara V.M.
da S. Martins, Alessandra
Pires, Giovanna F.F.
Souza, Felipe A.R.
Amorim, Camila C.
Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title_full Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title_fullStr Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title_full_unstemmed Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title_short Metagenomic analysis of MWWTP effluent treated via solar photo-Fenton at neutral pH: Effects upon microbial community, priority pathogens, and antibiotic resistance genes
title_sort metagenomic analysis of mwwtp effluent treated via solar photo-fenton at neutral ph: effects upon microbial community, priority pathogens, and antibiotic resistance genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573595/
https://www.ncbi.nlm.nih.gov/pubmed/34467925
http://dx.doi.org/10.1016/j.scitotenv.2021.149599
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