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Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis

Lupus nephritis (LN) is a common and severe organ manifestation of systemic lupus erythematosus (SLE) and is a major cause of SLE related deaths. Early diagnosis is essential to improve the prognosis of patients with LN. To screen the potential biomarkers associated with LN, we downloaded the gene e...

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Autores principales: Shen, Lingling, Lan, Lan, Zhu, Tingting, Chen, Hongjun, Gu, Haifeng, Wang, Cuili, Chen, Ying, Wang, Minmin, Tu, Haiyan, Enghard, Philipp, Jiang, Hong, Chen, Jianghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574154/
https://www.ncbi.nlm.nih.gov/pubmed/34760904
http://dx.doi.org/10.3389/fmed.2021.762848
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author Shen, Lingling
Lan, Lan
Zhu, Tingting
Chen, Hongjun
Gu, Haifeng
Wang, Cuili
Chen, Ying
Wang, Minmin
Tu, Haiyan
Enghard, Philipp
Jiang, Hong
Chen, Jianghua
author_facet Shen, Lingling
Lan, Lan
Zhu, Tingting
Chen, Hongjun
Gu, Haifeng
Wang, Cuili
Chen, Ying
Wang, Minmin
Tu, Haiyan
Enghard, Philipp
Jiang, Hong
Chen, Jianghua
author_sort Shen, Lingling
collection PubMed
description Lupus nephritis (LN) is a common and severe organ manifestation of systemic lupus erythematosus (SLE) and is a major cause of SLE related deaths. Early diagnosis is essential to improve the prognosis of patients with LN. To screen the potential biomarkers associated with LN, we downloaded the gene expression profile of GSE99967 from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was utilized to construct a gene co-expression network and identify gene modules associated with LN. Gene Ontology (GO) analysis was also applied to explore the biological function of genes and identify the key module. Differentially expressed genes (DEGs) were identified and Maximal Clique Centrality (MCC) values were calculated to screen hub genes. Furthermore, we selected promising biomarkers for real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) validation in independent cohorts. Our results indicated that five hub genes, including IFI44, IFIT3, HERC5, RSAD2, and DDX60 play vital roles in the pathogenesis of LN. Importantly, IFI44 may considered as a key biomarker in LN for its diagnostic capabilities, which is also a promising therapeutic target in the future.
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spelling pubmed-85741542021-11-09 Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis Shen, Lingling Lan, Lan Zhu, Tingting Chen, Hongjun Gu, Haifeng Wang, Cuili Chen, Ying Wang, Minmin Tu, Haiyan Enghard, Philipp Jiang, Hong Chen, Jianghua Front Med (Lausanne) Medicine Lupus nephritis (LN) is a common and severe organ manifestation of systemic lupus erythematosus (SLE) and is a major cause of SLE related deaths. Early diagnosis is essential to improve the prognosis of patients with LN. To screen the potential biomarkers associated with LN, we downloaded the gene expression profile of GSE99967 from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was utilized to construct a gene co-expression network and identify gene modules associated with LN. Gene Ontology (GO) analysis was also applied to explore the biological function of genes and identify the key module. Differentially expressed genes (DEGs) were identified and Maximal Clique Centrality (MCC) values were calculated to screen hub genes. Furthermore, we selected promising biomarkers for real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) validation in independent cohorts. Our results indicated that five hub genes, including IFI44, IFIT3, HERC5, RSAD2, and DDX60 play vital roles in the pathogenesis of LN. Importantly, IFI44 may considered as a key biomarker in LN for its diagnostic capabilities, which is also a promising therapeutic target in the future. Frontiers Media S.A. 2021-10-25 /pmc/articles/PMC8574154/ /pubmed/34760904 http://dx.doi.org/10.3389/fmed.2021.762848 Text en Copyright © 2021 Shen, Lan, Zhu, Chen, Gu, Wang, Chen, Wang, Tu, Enghard, Jiang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Shen, Lingling
Lan, Lan
Zhu, Tingting
Chen, Hongjun
Gu, Haifeng
Wang, Cuili
Chen, Ying
Wang, Minmin
Tu, Haiyan
Enghard, Philipp
Jiang, Hong
Chen, Jianghua
Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title_full Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title_fullStr Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title_full_unstemmed Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title_short Identification and Validation of IFI44 as Key Biomarker in Lupus Nephritis
title_sort identification and validation of ifi44 as key biomarker in lupus nephritis
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574154/
https://www.ncbi.nlm.nih.gov/pubmed/34760904
http://dx.doi.org/10.3389/fmed.2021.762848
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