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Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation
Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adul...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576262/ https://www.ncbi.nlm.nih.gov/pubmed/34731235 http://dx.doi.org/10.1242/bio.058910 |
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author | Shi, Lin Kong, Ruiyan Li, Zhengran Zhao, Hang Ma, Rui Bai, Guang Li, Jing Li, Zhouhua |
author_facet | Shi, Lin Kong, Ruiyan Li, Zhengran Zhao, Hang Ma, Rui Bai, Guang Li, Jing Li, Zhouhua |
author_sort | Shi, Lin |
collection | PubMed |
description | Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor. Interestingly, the phenotype of stum 9-3 mutant is not caused by disruption of stumble (stum), where the p-element is inserted. Detailed mapping, rescue experiments and mutant characterization show that stum 9-3 is a new allele of O-fucosyltransferase 1 (O-fut1). Our results indicate that unexpected mutants with interesting phenotype could be recovered in forward genetic screens using known p-element insertion stocks. |
format | Online Article Text |
id | pubmed-8576262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-85762622021-11-09 Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation Shi, Lin Kong, Ruiyan Li, Zhengran Zhao, Hang Ma, Rui Bai, Guang Li, Jing Li, Zhouhua Biol Open Research Article Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor. Interestingly, the phenotype of stum 9-3 mutant is not caused by disruption of stumble (stum), where the p-element is inserted. Detailed mapping, rescue experiments and mutant characterization show that stum 9-3 is a new allele of O-fucosyltransferase 1 (O-fut1). Our results indicate that unexpected mutants with interesting phenotype could be recovered in forward genetic screens using known p-element insertion stocks. The Company of Biologists Ltd 2021-11-03 /pmc/articles/PMC8576262/ /pubmed/34731235 http://dx.doi.org/10.1242/bio.058910 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Shi, Lin Kong, Ruiyan Li, Zhengran Zhao, Hang Ma, Rui Bai, Guang Li, Jing Li, Zhouhua Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title_full | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title_fullStr | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title_full_unstemmed | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title_short | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation |
title_sort | identification of a new allele of o-fucosyltransferase 1 involved in drosophila intestinal stem cell regulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576262/ https://www.ncbi.nlm.nih.gov/pubmed/34731235 http://dx.doi.org/10.1242/bio.058910 |
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