Cargando…

Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation

BACKGROUND: This article aims to explore whether ursolic acid (UA) inhibits the progression of retinoblastoma (Rb) by regulating stearoyl-CoA desaturase (SCD). METHODS: The Gene Expression Omnibus (GEO) database was used to filter the chip, then the GEO2R software was used to analyze the microarray...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Dan, Bao, Qi, Fu, Songbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576664/
https://www.ncbi.nlm.nih.gov/pubmed/34790754
http://dx.doi.org/10.21037/atm-21-4617
_version_ 1784595921858723840
author Zhou, Dan
Bao, Qi
Fu, Songbin
author_facet Zhou, Dan
Bao, Qi
Fu, Songbin
author_sort Zhou, Dan
collection PubMed
description BACKGROUND: This article aims to explore whether ursolic acid (UA) inhibits the progression of retinoblastoma (Rb) by regulating stearoyl-CoA desaturase (SCD). METHODS: The Gene Expression Omnibus (GEO) database was used to filter the chip, then the GEO2R software was used to analyze the microarray data (GSE97508, GSE24673, and GSE110811). Gene set enrichment analysis (GSEA) was used to analyze the relationship between the expression level of SCD and the proliferation, migration, invasion, and inflammation in Rb patients. SO-RB50 and Y79 cell proliferation, migration, and invasion were assessed by the CCK-8 assay, the colony formation assay, the Transwell assay, and the wound scratch test. The protein expression levels of SCD were measured by western blot. The mRNA expression levels of IL-8, IL-6, CXCL1, and CCL2 were measured by RT-qPCR. The protein expression levels of IL-8 and IL-6 were measured by ELISA. A xenograft nude mouse model was established to evaluate the effect of UA on tumor growth in male BALB/c mice. RESULTS: The expression levels of SCD were related to cell proliferation, migration, invasion, and inflammation. UA inhibited SO-RB50 and Y79 cell proliferation, migration, and invasion. At the same time, UA suppressed tumor growth in the xenograft nude mouse model. Overexpression of SCD promoted SO-RB50 and Y79 cell proliferation, migration, invasion, and inflammation, while SCD knockout inhibited SO-RB50 and Y79 cell proliferation, migration, invasion, and inflammation. Importantly, UA inhibited the proliferation, migration, and invasion of Rb cells through SCD inhibition. CONCLUSIONS: UA inhibited the proliferation, migration, and invasion of Rb cells through SCD. This provides a new scientific basis for targeted therapy of Rb.
format Online
Article
Text
id pubmed-8576664
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-85766642021-11-16 Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation Zhou, Dan Bao, Qi Fu, Songbin Ann Transl Med Original Article BACKGROUND: This article aims to explore whether ursolic acid (UA) inhibits the progression of retinoblastoma (Rb) by regulating stearoyl-CoA desaturase (SCD). METHODS: The Gene Expression Omnibus (GEO) database was used to filter the chip, then the GEO2R software was used to analyze the microarray data (GSE97508, GSE24673, and GSE110811). Gene set enrichment analysis (GSEA) was used to analyze the relationship between the expression level of SCD and the proliferation, migration, invasion, and inflammation in Rb patients. SO-RB50 and Y79 cell proliferation, migration, and invasion were assessed by the CCK-8 assay, the colony formation assay, the Transwell assay, and the wound scratch test. The protein expression levels of SCD were measured by western blot. The mRNA expression levels of IL-8, IL-6, CXCL1, and CCL2 were measured by RT-qPCR. The protein expression levels of IL-8 and IL-6 were measured by ELISA. A xenograft nude mouse model was established to evaluate the effect of UA on tumor growth in male BALB/c mice. RESULTS: The expression levels of SCD were related to cell proliferation, migration, invasion, and inflammation. UA inhibited SO-RB50 and Y79 cell proliferation, migration, and invasion. At the same time, UA suppressed tumor growth in the xenograft nude mouse model. Overexpression of SCD promoted SO-RB50 and Y79 cell proliferation, migration, invasion, and inflammation, while SCD knockout inhibited SO-RB50 and Y79 cell proliferation, migration, invasion, and inflammation. Importantly, UA inhibited the proliferation, migration, and invasion of Rb cells through SCD inhibition. CONCLUSIONS: UA inhibited the proliferation, migration, and invasion of Rb cells through SCD. This provides a new scientific basis for targeted therapy of Rb. AME Publishing Company 2021-10 /pmc/articles/PMC8576664/ /pubmed/34790754 http://dx.doi.org/10.21037/atm-21-4617 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Zhou, Dan
Bao, Qi
Fu, Songbin
Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title_full Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title_fullStr Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title_full_unstemmed Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title_short Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
title_sort anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576664/
https://www.ncbi.nlm.nih.gov/pubmed/34790754
http://dx.doi.org/10.21037/atm-21-4617
work_keys_str_mv AT zhoudan anticanceractivityofursolicacidonretinoblastomacellsdeterminedbybioinformaticsanalysisandvalidation
AT baoqi anticanceractivityofursolicacidonretinoblastomacellsdeterminedbybioinformaticsanalysisandvalidation
AT fusongbin anticanceractivityofursolicacidonretinoblastomacellsdeterminedbybioinformaticsanalysisandvalidation