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Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

BACKGROUND: Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective mi...

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Autores principales: Guo, Beiyi, Zeng, Songjun, Yin, Yuying, Li, Lin, Ma, Guohua, Wu, Kunlin, Fang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576892/
https://www.ncbi.nlm.nih.gov/pubmed/34749655
http://dx.doi.org/10.1186/s12864-021-08087-y
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author Guo, Beiyi
Zeng, Songjun
Yin, Yuying
Li, Lin
Ma, Guohua
Wu, Kunlin
Fang, Lin
author_facet Guo, Beiyi
Zeng, Songjun
Yin, Yuying
Li, Lin
Ma, Guohua
Wu, Kunlin
Fang, Lin
author_sort Guo, Beiyi
collection PubMed
description BACKGROUND: Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum. RESULTS: A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development. Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail. CONCLUSION: This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08087-y.
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spelling pubmed-85768922021-11-10 Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum Guo, Beiyi Zeng, Songjun Yin, Yuying Li, Lin Ma, Guohua Wu, Kunlin Fang, Lin BMC Genomics Research Article BACKGROUND: Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum. RESULTS: A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development. Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail. CONCLUSION: This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08087-y. BioMed Central 2021-11-08 /pmc/articles/PMC8576892/ /pubmed/34749655 http://dx.doi.org/10.1186/s12864-021-08087-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Guo, Beiyi
Zeng, Songjun
Yin, Yuying
Li, Lin
Ma, Guohua
Wu, Kunlin
Fang, Lin
Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_full Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_fullStr Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_full_unstemmed Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_short Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_sort characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of paphiopedilum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576892/
https://www.ncbi.nlm.nih.gov/pubmed/34749655
http://dx.doi.org/10.1186/s12864-021-08087-y
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