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Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma
The resistance of highly aggressive glioblastoma multiforme (GBM) to chemotherapy is a major clinical problem resulting in a poor prognosis. GBM contains a rare population of self-renewing cancer stem cells (CSCs) that proliferate, spurring the growth of new tumors, and evade chemotherapy. In cancer...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577150/ https://www.ncbi.nlm.nih.gov/pubmed/34742152 http://dx.doi.org/10.1016/j.tranon.2021.101255 |
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author | Noh, Kum Hee Lee, Song-Hee Lee, Haeri Jeong, Ae Jin Kim, Kyu Oh Shin, Hyun Mu Kim, Hang-Rae Park, Myung-Jin Park, Jong Bae Lee, Jiyoung Ye, Sang-Kyu |
author_facet | Noh, Kum Hee Lee, Song-Hee Lee, Haeri Jeong, Ae Jin Kim, Kyu Oh Shin, Hyun Mu Kim, Hang-Rae Park, Myung-Jin Park, Jong Bae Lee, Jiyoung Ye, Sang-Kyu |
author_sort | Noh, Kum Hee |
collection | PubMed |
description | The resistance of highly aggressive glioblastoma multiforme (GBM) to chemotherapy is a major clinical problem resulting in a poor prognosis. GBM contains a rare population of self-renewing cancer stem cells (CSCs) that proliferate, spurring the growth of new tumors, and evade chemotherapy. In cancer, major vault protein (MVP) is thought to contribute to drug resistance. However, the role of MVP as CSCs marker remains unknown and whether MVP could sensitize GBM cells to Temozolomide (TMZ) also is unclear. We found that sensitivity to TMZ was suppressed by significantly increasing the MVP expression in GBM cells with TMZ resistance. Also, MVP was associated with the expression of other multidrug-resistant proteins in tumorsphere of TMZ-resistant GBM cell, and was highly co-expressed with CSC markers in tumorsphere culture. On the other hands, knockdown of MVP resulted in reduced sphere formation and invasive capacity. Moreover, high expression of MVP was associated with tumor malignancy and survival rate in glioblastoma patients. Our study describes that MVP is a potentially novel maker for glioblastoma stem cells and may be useful as a target for preventing TMZ resistance in GBM patients. |
format | Online Article Text |
id | pubmed-8577150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-85771502021-11-19 Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma Noh, Kum Hee Lee, Song-Hee Lee, Haeri Jeong, Ae Jin Kim, Kyu Oh Shin, Hyun Mu Kim, Hang-Rae Park, Myung-Jin Park, Jong Bae Lee, Jiyoung Ye, Sang-Kyu Transl Oncol Original Research The resistance of highly aggressive glioblastoma multiforme (GBM) to chemotherapy is a major clinical problem resulting in a poor prognosis. GBM contains a rare population of self-renewing cancer stem cells (CSCs) that proliferate, spurring the growth of new tumors, and evade chemotherapy. In cancer, major vault protein (MVP) is thought to contribute to drug resistance. However, the role of MVP as CSCs marker remains unknown and whether MVP could sensitize GBM cells to Temozolomide (TMZ) also is unclear. We found that sensitivity to TMZ was suppressed by significantly increasing the MVP expression in GBM cells with TMZ resistance. Also, MVP was associated with the expression of other multidrug-resistant proteins in tumorsphere of TMZ-resistant GBM cell, and was highly co-expressed with CSC markers in tumorsphere culture. On the other hands, knockdown of MVP resulted in reduced sphere formation and invasive capacity. Moreover, high expression of MVP was associated with tumor malignancy and survival rate in glioblastoma patients. Our study describes that MVP is a potentially novel maker for glioblastoma stem cells and may be useful as a target for preventing TMZ resistance in GBM patients. Neoplasia Press 2021-11-02 /pmc/articles/PMC8577150/ /pubmed/34742152 http://dx.doi.org/10.1016/j.tranon.2021.101255 Text en © 2021 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Noh, Kum Hee Lee, Song-Hee Lee, Haeri Jeong, Ae Jin Kim, Kyu Oh Shin, Hyun Mu Kim, Hang-Rae Park, Myung-Jin Park, Jong Bae Lee, Jiyoung Ye, Sang-Kyu Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title | Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title_full | Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title_fullStr | Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title_full_unstemmed | Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title_short | Novel cancer stem cell marker MVP enhances temozolomide-resistance in glioblastoma |
title_sort | novel cancer stem cell marker mvp enhances temozolomide-resistance in glioblastoma |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577150/ https://www.ncbi.nlm.nih.gov/pubmed/34742152 http://dx.doi.org/10.1016/j.tranon.2021.101255 |
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