A live-imaging protocol to track cell movement in the Xenopus embryo

Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells...

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Detalles Bibliográficos
Autores principales: Chuyen, Alexandre, Daian, Fabrice, Pasini, Andrea, Kodjabachian, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577151/
https://www.ncbi.nlm.nih.gov/pubmed/34778847
http://dx.doi.org/10.1016/j.xpro.2021.100928
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author Chuyen, Alexandre
Daian, Fabrice
Pasini, Andrea
Kodjabachian, Laurent
author_facet Chuyen, Alexandre
Daian, Fabrice
Pasini, Andrea
Kodjabachian, Laurent
author_sort Chuyen, Alexandre
collection PubMed
description Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded. For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021).
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spelling pubmed-85771512021-11-12 A live-imaging protocol to track cell movement in the Xenopus embryo Chuyen, Alexandre Daian, Fabrice Pasini, Andrea Kodjabachian, Laurent STAR Protoc Protocol Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded. For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021). Elsevier 2021-11-02 /pmc/articles/PMC8577151/ /pubmed/34778847 http://dx.doi.org/10.1016/j.xpro.2021.100928 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chuyen, Alexandre
Daian, Fabrice
Pasini, Andrea
Kodjabachian, Laurent
A live-imaging protocol to track cell movement in the Xenopus embryo
title A live-imaging protocol to track cell movement in the Xenopus embryo
title_full A live-imaging protocol to track cell movement in the Xenopus embryo
title_fullStr A live-imaging protocol to track cell movement in the Xenopus embryo
title_full_unstemmed A live-imaging protocol to track cell movement in the Xenopus embryo
title_short A live-imaging protocol to track cell movement in the Xenopus embryo
title_sort live-imaging protocol to track cell movement in the xenopus embryo
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577151/
https://www.ncbi.nlm.nih.gov/pubmed/34778847
http://dx.doi.org/10.1016/j.xpro.2021.100928
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