Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs i...

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Detalles Bibliográficos
Autores principales: Jovasevic, Vladimir, Zhang, Hui, Petrovic, Zorica, Cicvaric, Ana, Radulovic, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577157/
https://www.ncbi.nlm.nih.gov/pubmed/34778848
http://dx.doi.org/10.1016/j.xpro.2021.100931
Descripción
Sumario:Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization. For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021).

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