Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs i...

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Autores principales: Jovasevic, Vladimir, Zhang, Hui, Petrovic, Zorica, Cicvaric, Ana, Radulovic, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577157/
https://www.ncbi.nlm.nih.gov/pubmed/34778848
http://dx.doi.org/10.1016/j.xpro.2021.100931
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author Jovasevic, Vladimir
Zhang, Hui
Petrovic, Zorica
Cicvaric, Ana
Radulovic, Jelena
author_facet Jovasevic, Vladimir
Zhang, Hui
Petrovic, Zorica
Cicvaric, Ana
Radulovic, Jelena
author_sort Jovasevic, Vladimir
collection PubMed
description Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization. For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021).
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spelling pubmed-85771572021-11-12 Protocol for assessing the role of hippocampal perineuronal nets in aversive memories Jovasevic, Vladimir Zhang, Hui Petrovic, Zorica Cicvaric, Ana Radulovic, Jelena STAR Protoc Protocol Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization. For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021). Elsevier 2021-11-02 /pmc/articles/PMC8577157/ /pubmed/34778848 http://dx.doi.org/10.1016/j.xpro.2021.100931 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jovasevic, Vladimir
Zhang, Hui
Petrovic, Zorica
Cicvaric, Ana
Radulovic, Jelena
Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_full Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_fullStr Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_full_unstemmed Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_short Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_sort protocol for assessing the role of hippocampal perineuronal nets in aversive memories
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577157/
https://www.ncbi.nlm.nih.gov/pubmed/34778848
http://dx.doi.org/10.1016/j.xpro.2021.100931
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