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Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effect...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577801/ https://www.ncbi.nlm.nih.gov/pubmed/34765669 http://dx.doi.org/10.3389/fvets.2021.756534 |
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author | Xiong, Zhiwei Xing, Chenghong Xu, Tianfang Yang, Yan Liu, Guohui Hu, Guoliang Cao, Huabin Zhang, Caiying Guo, Xiaoquan Yang, Fan |
author_facet | Xiong, Zhiwei Xing, Chenghong Xu, Tianfang Yang, Yan Liu, Guohui Hu, Guoliang Cao, Huabin Zhang, Caiying Guo, Xiaoquan Yang, Fan |
author_sort | Xiong, Zhiwei |
collection | PubMed |
description | Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effects of excessive V on oxidative stress and MQC in duck hearts, 72 ducks were randomly divided into two groups, including the control group and the V group (30 mg of V/kg dry matter). The cardiac tissues were collected for the histomorphology observation and oxidative stress status evaluation at 22 and 44 days. In addition, the mRNA and protein levels of MQC-related factors were also analyzed. The results showed that excessive V could trigger vacuolar degeneration, granular degeneration, as well as mitochondrial vacuolization and swelling in myocardial cells. In addition, CAT activity was elevated in two time points, while T-SOD activity was increased in 22 days but decreased in 44 days after V treatment. Meanwhile, excessive V intake could also increase the number of Drp1 puncta, the mRNA levels of mitochondrial fission–related factors (Drp1and MFF), and protein (MFF) level, but decrease the number of Parkin puncta and the mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), mitochondrial fusion (OPA1, Mfn1, and Mfn2), and mitophagy (Parkin, PINK1, P62, and LC3B) related mRNA levels and protein (PGC-1α, Mfn1, Mfn2, PINK1) levels. Collectively, our results suggested that excessive V could induce oxidative stress and MQC disorder in the heart of ducks. |
format | Online Article Text |
id | pubmed-8577801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85778012021-11-10 Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks Xiong, Zhiwei Xing, Chenghong Xu, Tianfang Yang, Yan Liu, Guohui Hu, Guoliang Cao, Huabin Zhang, Caiying Guo, Xiaoquan Yang, Fan Front Vet Sci Veterinary Science Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effects of excessive V on oxidative stress and MQC in duck hearts, 72 ducks were randomly divided into two groups, including the control group and the V group (30 mg of V/kg dry matter). The cardiac tissues were collected for the histomorphology observation and oxidative stress status evaluation at 22 and 44 days. In addition, the mRNA and protein levels of MQC-related factors were also analyzed. The results showed that excessive V could trigger vacuolar degeneration, granular degeneration, as well as mitochondrial vacuolization and swelling in myocardial cells. In addition, CAT activity was elevated in two time points, while T-SOD activity was increased in 22 days but decreased in 44 days after V treatment. Meanwhile, excessive V intake could also increase the number of Drp1 puncta, the mRNA levels of mitochondrial fission–related factors (Drp1and MFF), and protein (MFF) level, but decrease the number of Parkin puncta and the mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), mitochondrial fusion (OPA1, Mfn1, and Mfn2), and mitophagy (Parkin, PINK1, P62, and LC3B) related mRNA levels and protein (PGC-1α, Mfn1, Mfn2, PINK1) levels. Collectively, our results suggested that excessive V could induce oxidative stress and MQC disorder in the heart of ducks. Frontiers Media S.A. 2021-10-26 /pmc/articles/PMC8577801/ /pubmed/34765669 http://dx.doi.org/10.3389/fvets.2021.756534 Text en Copyright © 2021 Xiong, Xing, Xu, Yang, Liu, Hu, Cao, Zhang, Guo and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Xiong, Zhiwei Xing, Chenghong Xu, Tianfang Yang, Yan Liu, Guohui Hu, Guoliang Cao, Huabin Zhang, Caiying Guo, Xiaoquan Yang, Fan Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title | Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title_full | Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title_fullStr | Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title_full_unstemmed | Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title_short | Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks |
title_sort | vanadium induces oxidative stress and mitochondrial quality control disorder in the heart of ducks |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577801/ https://www.ncbi.nlm.nih.gov/pubmed/34765669 http://dx.doi.org/10.3389/fvets.2021.756534 |
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