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Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks

Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effect...

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Autores principales: Xiong, Zhiwei, Xing, Chenghong, Xu, Tianfang, Yang, Yan, Liu, Guohui, Hu, Guoliang, Cao, Huabin, Zhang, Caiying, Guo, Xiaoquan, Yang, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577801/
https://www.ncbi.nlm.nih.gov/pubmed/34765669
http://dx.doi.org/10.3389/fvets.2021.756534
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author Xiong, Zhiwei
Xing, Chenghong
Xu, Tianfang
Yang, Yan
Liu, Guohui
Hu, Guoliang
Cao, Huabin
Zhang, Caiying
Guo, Xiaoquan
Yang, Fan
author_facet Xiong, Zhiwei
Xing, Chenghong
Xu, Tianfang
Yang, Yan
Liu, Guohui
Hu, Guoliang
Cao, Huabin
Zhang, Caiying
Guo, Xiaoquan
Yang, Fan
author_sort Xiong, Zhiwei
collection PubMed
description Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effects of excessive V on oxidative stress and MQC in duck hearts, 72 ducks were randomly divided into two groups, including the control group and the V group (30 mg of V/kg dry matter). The cardiac tissues were collected for the histomorphology observation and oxidative stress status evaluation at 22 and 44 days. In addition, the mRNA and protein levels of MQC-related factors were also analyzed. The results showed that excessive V could trigger vacuolar degeneration, granular degeneration, as well as mitochondrial vacuolization and swelling in myocardial cells. In addition, CAT activity was elevated in two time points, while T-SOD activity was increased in 22 days but decreased in 44 days after V treatment. Meanwhile, excessive V intake could also increase the number of Drp1 puncta, the mRNA levels of mitochondrial fission–related factors (Drp1and MFF), and protein (MFF) level, but decrease the number of Parkin puncta and the mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), mitochondrial fusion (OPA1, Mfn1, and Mfn2), and mitophagy (Parkin, PINK1, P62, and LC3B) related mRNA levels and protein (PGC-1α, Mfn1, Mfn2, PINK1) levels. Collectively, our results suggested that excessive V could induce oxidative stress and MQC disorder in the heart of ducks.
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spelling pubmed-85778012021-11-10 Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks Xiong, Zhiwei Xing, Chenghong Xu, Tianfang Yang, Yan Liu, Guohui Hu, Guoliang Cao, Huabin Zhang, Caiying Guo, Xiaoquan Yang, Fan Front Vet Sci Veterinary Science Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effects of excessive V on oxidative stress and MQC in duck hearts, 72 ducks were randomly divided into two groups, including the control group and the V group (30 mg of V/kg dry matter). The cardiac tissues were collected for the histomorphology observation and oxidative stress status evaluation at 22 and 44 days. In addition, the mRNA and protein levels of MQC-related factors were also analyzed. The results showed that excessive V could trigger vacuolar degeneration, granular degeneration, as well as mitochondrial vacuolization and swelling in myocardial cells. In addition, CAT activity was elevated in two time points, while T-SOD activity was increased in 22 days but decreased in 44 days after V treatment. Meanwhile, excessive V intake could also increase the number of Drp1 puncta, the mRNA levels of mitochondrial fission–related factors (Drp1and MFF), and protein (MFF) level, but decrease the number of Parkin puncta and the mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), mitochondrial fusion (OPA1, Mfn1, and Mfn2), and mitophagy (Parkin, PINK1, P62, and LC3B) related mRNA levels and protein (PGC-1α, Mfn1, Mfn2, PINK1) levels. Collectively, our results suggested that excessive V could induce oxidative stress and MQC disorder in the heart of ducks. Frontiers Media S.A. 2021-10-26 /pmc/articles/PMC8577801/ /pubmed/34765669 http://dx.doi.org/10.3389/fvets.2021.756534 Text en Copyright © 2021 Xiong, Xing, Xu, Yang, Liu, Hu, Cao, Zhang, Guo and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Xiong, Zhiwei
Xing, Chenghong
Xu, Tianfang
Yang, Yan
Liu, Guohui
Hu, Guoliang
Cao, Huabin
Zhang, Caiying
Guo, Xiaoquan
Yang, Fan
Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title_full Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title_fullStr Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title_full_unstemmed Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title_short Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks
title_sort vanadium induces oxidative stress and mitochondrial quality control disorder in the heart of ducks
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577801/
https://www.ncbi.nlm.nih.gov/pubmed/34765669
http://dx.doi.org/10.3389/fvets.2021.756534
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