Cargando…
Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden S...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Investigative Pathology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577872/ https://www.ncbi.nlm.nih.gov/pubmed/34767812 http://dx.doi.org/10.1016/j.ajpath.2021.10.009 |
_version_ | 1784596151556636672 |
---|---|
author | Mulka, Kathleen R. Beck, Sarah E. Solis, Clarisse V. Johanson, Andrew L. Queen, Suzanne E. McCarron, Megan E. Richardson, Morgan R. Zhou, Ruifeng Marinho, Paula Jedlicka, Anne Guerrero-Martin, Selena Shirk, Erin N. Braxton, Alicia M. Brockhurst, Jacqueline Creisher, Patrick S. Dhakal, Santosh Brayton, Cory F. Veenhuis, Rebecca T. Metcalf Pate, Kelly A. Karakousis, Petros C. Zahnow, Cynthia A. Klein, Sabra L. Jain, Sanjay K. Tarwater, Patrick M. Pekosz, Andrew S. Villano, Jason S. Mankowski, Joseph L. |
author_facet | Mulka, Kathleen R. Beck, Sarah E. Solis, Clarisse V. Johanson, Andrew L. Queen, Suzanne E. McCarron, Megan E. Richardson, Morgan R. Zhou, Ruifeng Marinho, Paula Jedlicka, Anne Guerrero-Martin, Selena Shirk, Erin N. Braxton, Alicia M. Brockhurst, Jacqueline Creisher, Patrick S. Dhakal, Santosh Brayton, Cory F. Veenhuis, Rebecca T. Metcalf Pate, Kelly A. Karakousis, Petros C. Zahnow, Cynthia A. Klein, Sabra L. Jain, Sanjay K. Tarwater, Patrick M. Pekosz, Andrew S. Villano, Jason S. Mankowski, Joseph L. |
author_sort | Mulka, Kathleen R. |
collection | PubMed |
description | To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2–inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model. |
format | Online Article Text |
id | pubmed-8577872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Investigative Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-85778722021-11-10 Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters Mulka, Kathleen R. Beck, Sarah E. Solis, Clarisse V. Johanson, Andrew L. Queen, Suzanne E. McCarron, Megan E. Richardson, Morgan R. Zhou, Ruifeng Marinho, Paula Jedlicka, Anne Guerrero-Martin, Selena Shirk, Erin N. Braxton, Alicia M. Brockhurst, Jacqueline Creisher, Patrick S. Dhakal, Santosh Brayton, Cory F. Veenhuis, Rebecca T. Metcalf Pate, Kelly A. Karakousis, Petros C. Zahnow, Cynthia A. Klein, Sabra L. Jain, Sanjay K. Tarwater, Patrick M. Pekosz, Andrew S. Villano, Jason S. Mankowski, Joseph L. Am J Pathol Regular Article To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2–inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model. American Society for Investigative Pathology 2022-02 /pmc/articles/PMC8577872/ /pubmed/34767812 http://dx.doi.org/10.1016/j.ajpath.2021.10.009 Text en © 2022 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
spellingShingle | Regular Article Mulka, Kathleen R. Beck, Sarah E. Solis, Clarisse V. Johanson, Andrew L. Queen, Suzanne E. McCarron, Megan E. Richardson, Morgan R. Zhou, Ruifeng Marinho, Paula Jedlicka, Anne Guerrero-Martin, Selena Shirk, Erin N. Braxton, Alicia M. Brockhurst, Jacqueline Creisher, Patrick S. Dhakal, Santosh Brayton, Cory F. Veenhuis, Rebecca T. Metcalf Pate, Kelly A. Karakousis, Petros C. Zahnow, Cynthia A. Klein, Sabra L. Jain, Sanjay K. Tarwater, Patrick M. Pekosz, Andrew S. Villano, Jason S. Mankowski, Joseph L. Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title | Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title_full | Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title_fullStr | Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title_full_unstemmed | Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title_short | Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters |
title_sort | progression and resolution of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) infection in golden syrian hamsters |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577872/ https://www.ncbi.nlm.nih.gov/pubmed/34767812 http://dx.doi.org/10.1016/j.ajpath.2021.10.009 |
work_keys_str_mv | AT mulkakathleenr progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT becksarahe progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT solisclarissev progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT johansonandrewl progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT queensuzannee progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT mccarronmegane progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT richardsonmorganr progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT zhouruifeng progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT marinhopaula progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT jedlickaanne progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT guerreromartinselena progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT shirkerinn progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT braxtonaliciam progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT brockhurstjacqueline progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT creisherpatricks progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT dhakalsantosh progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT braytoncoryf progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT veenhuisrebeccat progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT metcalfpatekellya progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT karakousispetrosc progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT zahnowcynthiaa progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT kleinsabral progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT jainsanjayk progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT tarwaterpatrickm progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT pekoszandrews progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT villanojasons progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT mankowskijosephl progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters AT progressionandresolutionofsevereacuterespiratorysyndromecoronavirus2sarscov2infectioningoldensyrianhamsters |