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Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters

To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden S...

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Autores principales: Mulka, Kathleen R., Beck, Sarah E., Solis, Clarisse V., Johanson, Andrew L., Queen, Suzanne E., McCarron, Megan E., Richardson, Morgan R., Zhou, Ruifeng, Marinho, Paula, Jedlicka, Anne, Guerrero-Martin, Selena, Shirk, Erin N., Braxton, Alicia M., Brockhurst, Jacqueline, Creisher, Patrick S., Dhakal, Santosh, Brayton, Cory F., Veenhuis, Rebecca T., Metcalf Pate, Kelly A., Karakousis, Petros C., Zahnow, Cynthia A., Klein, Sabra L., Jain, Sanjay K., Tarwater, Patrick M., Pekosz, Andrew S., Villano, Jason S., Mankowski, Joseph L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Investigative Pathology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577872/
https://www.ncbi.nlm.nih.gov/pubmed/34767812
http://dx.doi.org/10.1016/j.ajpath.2021.10.009
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author Mulka, Kathleen R.
Beck, Sarah E.
Solis, Clarisse V.
Johanson, Andrew L.
Queen, Suzanne E.
McCarron, Megan E.
Richardson, Morgan R.
Zhou, Ruifeng
Marinho, Paula
Jedlicka, Anne
Guerrero-Martin, Selena
Shirk, Erin N.
Braxton, Alicia M.
Brockhurst, Jacqueline
Creisher, Patrick S.
Dhakal, Santosh
Brayton, Cory F.
Veenhuis, Rebecca T.
Metcalf Pate, Kelly A.
Karakousis, Petros C.
Zahnow, Cynthia A.
Klein, Sabra L.
Jain, Sanjay K.
Tarwater, Patrick M.
Pekosz, Andrew S.
Villano, Jason S.
Mankowski, Joseph L.
author_facet Mulka, Kathleen R.
Beck, Sarah E.
Solis, Clarisse V.
Johanson, Andrew L.
Queen, Suzanne E.
McCarron, Megan E.
Richardson, Morgan R.
Zhou, Ruifeng
Marinho, Paula
Jedlicka, Anne
Guerrero-Martin, Selena
Shirk, Erin N.
Braxton, Alicia M.
Brockhurst, Jacqueline
Creisher, Patrick S.
Dhakal, Santosh
Brayton, Cory F.
Veenhuis, Rebecca T.
Metcalf Pate, Kelly A.
Karakousis, Petros C.
Zahnow, Cynthia A.
Klein, Sabra L.
Jain, Sanjay K.
Tarwater, Patrick M.
Pekosz, Andrew S.
Villano, Jason S.
Mankowski, Joseph L.
author_sort Mulka, Kathleen R.
collection PubMed
description To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2–inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model.
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spelling pubmed-85778722021-11-10 Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters Mulka, Kathleen R. Beck, Sarah E. Solis, Clarisse V. Johanson, Andrew L. Queen, Suzanne E. McCarron, Megan E. Richardson, Morgan R. Zhou, Ruifeng Marinho, Paula Jedlicka, Anne Guerrero-Martin, Selena Shirk, Erin N. Braxton, Alicia M. Brockhurst, Jacqueline Creisher, Patrick S. Dhakal, Santosh Brayton, Cory F. Veenhuis, Rebecca T. Metcalf Pate, Kelly A. Karakousis, Petros C. Zahnow, Cynthia A. Klein, Sabra L. Jain, Sanjay K. Tarwater, Patrick M. Pekosz, Andrew S. Villano, Jason S. Mankowski, Joseph L. Am J Pathol Regular Article To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2–inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model. American Society for Investigative Pathology 2022-02 /pmc/articles/PMC8577872/ /pubmed/34767812 http://dx.doi.org/10.1016/j.ajpath.2021.10.009 Text en © 2022 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
spellingShingle Regular Article
Mulka, Kathleen R.
Beck, Sarah E.
Solis, Clarisse V.
Johanson, Andrew L.
Queen, Suzanne E.
McCarron, Megan E.
Richardson, Morgan R.
Zhou, Ruifeng
Marinho, Paula
Jedlicka, Anne
Guerrero-Martin, Selena
Shirk, Erin N.
Braxton, Alicia M.
Brockhurst, Jacqueline
Creisher, Patrick S.
Dhakal, Santosh
Brayton, Cory F.
Veenhuis, Rebecca T.
Metcalf Pate, Kelly A.
Karakousis, Petros C.
Zahnow, Cynthia A.
Klein, Sabra L.
Jain, Sanjay K.
Tarwater, Patrick M.
Pekosz, Andrew S.
Villano, Jason S.
Mankowski, Joseph L.
Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title_full Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title_fullStr Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title_full_unstemmed Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title_short Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters
title_sort progression and resolution of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) infection in golden syrian hamsters
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577872/
https://www.ncbi.nlm.nih.gov/pubmed/34767812
http://dx.doi.org/10.1016/j.ajpath.2021.10.009
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