Cargando…
Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback
The accumulation of autotoxins and soilborne pathogens in soil was shown to be the primary driver of negative plant-soil feedback (NPSF). There is a concerted understanding that plants could enhance their adaptability to biotic or abiotic stress by modifying the rhizosphere microbiome. However, it i...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579924/ https://www.ncbi.nlm.nih.gov/pubmed/34756064 http://dx.doi.org/10.1128/Spectrum.01400-21 |
_version_ | 1784596519970668544 |
---|---|
author | Luo, Lifen Wang, Luotao Deng, Linmei Mei, Xinyue Liu, Yixiang Huang, Huichuan Du, Fei Zhu, Shusheng Yang, Min |
author_facet | Luo, Lifen Wang, Luotao Deng, Linmei Mei, Xinyue Liu, Yixiang Huang, Huichuan Du, Fei Zhu, Shusheng Yang, Min |
author_sort | Luo, Lifen |
collection | PubMed |
description | The accumulation of autotoxins and soilborne pathogens in soil was shown to be the primary driver of negative plant-soil feedback (NPSF). There is a concerted understanding that plants could enhance their adaptability to biotic or abiotic stress by modifying the rhizosphere microbiome. However, it is not clear whether autotoxins could enrich microbes to degrade themselves or antagonize soilborne pathogens. Here, we found that the microbiome degraded autotoxic ginsenosides, belonging to triterpenoid glycosides, and antagonized pathogens in the rhizosphere soil of Panax notoginseng (sanqi). Deep analysis by 16S rRNA sequencing showed that the bacterial community was obviously changed in the rhizosphere soil and identified the Burkholderia-Caballeronia-Paraburkholderia (BCP) group as the main ginsenoside-enriched bacteria in the rhizosphere soil. Eight strains belonging to the BCP group were isolated, and Burkholderia isolate B36 showed a high ability to simultaneously degrade autotoxic ginsenosides (Rb(1), Rg(1), and Rd) and antagonize the soilborne pathogen Ilyonectria destructans. Interestingly, ginsenosides could stimulate the growth and biofilm formation of B36, eventually enhancing the antagonistic ability of B36 to I. destructans and the colonization ability in the rhizosphere soil. In summary, autotoxic ginsenosides secreted by P. notoginseng could enrich beneficial microbes in the rhizosphere to simultaneously degrade autotoxins and antagonize pathogen, providing a novel ecological strategy to alleviate NPSF. IMPORTANCE Autotoxic ginsenosides, secreted by sanqi into soil, could enrich Burkholderia sp. to alleviate negative plant-soil feedback (NPSF) by degrading autotoxins and antagonizing the root rot pathogen. In detail, ginsenosides could stimulate the growth and biofilm formation of Burkholderia sp. B36, eventually enhancing the antagonistic ability of Burkholderia sp. B36 to a soilborne pathogen and the colonization of B36 in soil. This ecological strategy could alleviate NPSF by manipulating the rhizosphere microbiome to simultaneously degrade autotoxins and antagonize pathogen. |
format | Online Article Text |
id | pubmed-8579924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-85799242021-11-12 Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback Luo, Lifen Wang, Luotao Deng, Linmei Mei, Xinyue Liu, Yixiang Huang, Huichuan Du, Fei Zhu, Shusheng Yang, Min Microbiol Spectr Research Article The accumulation of autotoxins and soilborne pathogens in soil was shown to be the primary driver of negative plant-soil feedback (NPSF). There is a concerted understanding that plants could enhance their adaptability to biotic or abiotic stress by modifying the rhizosphere microbiome. However, it is not clear whether autotoxins could enrich microbes to degrade themselves or antagonize soilborne pathogens. Here, we found that the microbiome degraded autotoxic ginsenosides, belonging to triterpenoid glycosides, and antagonized pathogens in the rhizosphere soil of Panax notoginseng (sanqi). Deep analysis by 16S rRNA sequencing showed that the bacterial community was obviously changed in the rhizosphere soil and identified the Burkholderia-Caballeronia-Paraburkholderia (BCP) group as the main ginsenoside-enriched bacteria in the rhizosphere soil. Eight strains belonging to the BCP group were isolated, and Burkholderia isolate B36 showed a high ability to simultaneously degrade autotoxic ginsenosides (Rb(1), Rg(1), and Rd) and antagonize the soilborne pathogen Ilyonectria destructans. Interestingly, ginsenosides could stimulate the growth and biofilm formation of B36, eventually enhancing the antagonistic ability of B36 to I. destructans and the colonization ability in the rhizosphere soil. In summary, autotoxic ginsenosides secreted by P. notoginseng could enrich beneficial microbes in the rhizosphere to simultaneously degrade autotoxins and antagonize pathogen, providing a novel ecological strategy to alleviate NPSF. IMPORTANCE Autotoxic ginsenosides, secreted by sanqi into soil, could enrich Burkholderia sp. to alleviate negative plant-soil feedback (NPSF) by degrading autotoxins and antagonizing the root rot pathogen. In detail, ginsenosides could stimulate the growth and biofilm formation of Burkholderia sp. B36, eventually enhancing the antagonistic ability of Burkholderia sp. B36 to a soilborne pathogen and the colonization of B36 in soil. This ecological strategy could alleviate NPSF by manipulating the rhizosphere microbiome to simultaneously degrade autotoxins and antagonize pathogen. American Society for Microbiology 2021-11-10 /pmc/articles/PMC8579924/ /pubmed/34756064 http://dx.doi.org/10.1128/Spectrum.01400-21 Text en Copyright © 2021 Luo et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Luo, Lifen Wang, Luotao Deng, Linmei Mei, Xinyue Liu, Yixiang Huang, Huichuan Du, Fei Zhu, Shusheng Yang, Min Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title | Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title_full | Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title_fullStr | Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title_full_unstemmed | Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title_short | Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback |
title_sort | enrichment of burkholderia in the rhizosphere by autotoxic ginsenosides to alleviate negative plant-soil feedback |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579924/ https://www.ncbi.nlm.nih.gov/pubmed/34756064 http://dx.doi.org/10.1128/Spectrum.01400-21 |
work_keys_str_mv | AT luolifen enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT wangluotao enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT denglinmei enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT meixinyue enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT liuyixiang enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT huanghuichuan enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT dufei enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT zhushusheng enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback AT yangmin enrichmentofburkholderiaintherhizospherebyautotoxicginsenosidestoalleviatenegativeplantsoilfeedback |