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Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes
Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the ca...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580047/ https://www.ncbi.nlm.nih.gov/pubmed/34881008 http://dx.doi.org/10.1039/d1sc04430c |
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author | Zhu, Jinbo Yan, Zhiqiang Bošković, Filip Haynes, Cally J. E. Kieffer, Marion Greenfield, Jake L. Wang, Jin Nitschke, Jonathan R. Keyser, Ulrich F. |
author_facet | Zhu, Jinbo Yan, Zhiqiang Bošković, Filip Haynes, Cally J. E. Kieffer, Marion Greenfield, Jake L. Wang, Jin Nitschke, Jonathan R. Keyser, Ulrich F. |
author_sort | Zhu, Jinbo |
collection | PubMed |
description | Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA–cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5′ end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease. |
format | Online Article Text |
id | pubmed-8580047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-85800472021-12-07 Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes Zhu, Jinbo Yan, Zhiqiang Bošković, Filip Haynes, Cally J. E. Kieffer, Marion Greenfield, Jake L. Wang, Jin Nitschke, Jonathan R. Keyser, Ulrich F. Chem Sci Chemistry Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA–cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5′ end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease. The Royal Society of Chemistry 2021-10-07 /pmc/articles/PMC8580047/ /pubmed/34881008 http://dx.doi.org/10.1039/d1sc04430c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Zhu, Jinbo Yan, Zhiqiang Bošković, Filip Haynes, Cally J. E. Kieffer, Marion Greenfield, Jake L. Wang, Jin Nitschke, Jonathan R. Keyser, Ulrich F. Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title | Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title_full | Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title_fullStr | Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title_full_unstemmed | Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title_short | Fe(II)(4)L(4) tetrahedron binds and aggregates DNA G-quadruplexes |
title_sort | fe(ii)(4)l(4) tetrahedron binds and aggregates dna g-quadruplexes |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580047/ https://www.ncbi.nlm.nih.gov/pubmed/34881008 http://dx.doi.org/10.1039/d1sc04430c |
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