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Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India

BACKGROUND: Etiology of male infertility is intriguing and Y chromosome microdeletion within azoospermia factor (AZF) sub‐regions is considered major cause. We conducted a screening for Y chromosome microdeletion in an infertile male cohort from West Bengal, India to characterize Y chromosome microd...

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Autores principales: Dutta, Saurav, Paladhi, Pranab, Pal, Samudra, Bose, Gunja, Ghosh, Papiya, Chattopadhyay, Ratna, Chakravarty, Baidyanath, Ghosh, Sujay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580071/
https://www.ncbi.nlm.nih.gov/pubmed/34427986
http://dx.doi.org/10.1002/mgg3.1769
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author Dutta, Saurav
Paladhi, Pranab
Pal, Samudra
Bose, Gunja
Ghosh, Papiya
Chattopadhyay, Ratna
Chakravarty, Baidyanath
Ghosh, Sujay
author_facet Dutta, Saurav
Paladhi, Pranab
Pal, Samudra
Bose, Gunja
Ghosh, Papiya
Chattopadhyay, Ratna
Chakravarty, Baidyanath
Ghosh, Sujay
author_sort Dutta, Saurav
collection PubMed
description BACKGROUND: Etiology of male infertility is intriguing and Y chromosome microdeletion within azoospermia factor (AZF) sub‐regions is considered major cause. We conducted a screening for Y chromosome microdeletion in an infertile male cohort from West Bengal, India to characterize Y chromosome microdeletion among infertile men. METHODS: We recruited case subjects that were categorized on the basis of sperm count as azoospermia (N = 63), severe oligozoospermia (N = 38), and oligozoospermia (N = 17) and compared them with age, demography, and ethnicity matched healthy proven fertile control males (N = 84). Sequence Tagged Site makers and polymerase chain reaction based profiling of Y chromosome was done for AZF region and SRY for cases and controls. RESULTS: We scored 16.1% of cases (19 out of 118) that bear one or more microdeletions in the studied loci and none among the controls. The aberrations were more frequent among azoospermic males (17 of 19) than in severe oligozoospermic subjects (2 of 19). CONCLUSION: Our study provides the results of screening of the largest Bengali infertile men sample genotyped with the maximum number of STS markers spanning the entire length of Y chromosome long arm. Y chromosome microdeletion is a significant genetic etiology of infertility among Bengali men.
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spelling pubmed-85800712021-11-17 Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India Dutta, Saurav Paladhi, Pranab Pal, Samudra Bose, Gunja Ghosh, Papiya Chattopadhyay, Ratna Chakravarty, Baidyanath Ghosh, Sujay Mol Genet Genomic Med Original Articles BACKGROUND: Etiology of male infertility is intriguing and Y chromosome microdeletion within azoospermia factor (AZF) sub‐regions is considered major cause. We conducted a screening for Y chromosome microdeletion in an infertile male cohort from West Bengal, India to characterize Y chromosome microdeletion among infertile men. METHODS: We recruited case subjects that were categorized on the basis of sperm count as azoospermia (N = 63), severe oligozoospermia (N = 38), and oligozoospermia (N = 17) and compared them with age, demography, and ethnicity matched healthy proven fertile control males (N = 84). Sequence Tagged Site makers and polymerase chain reaction based profiling of Y chromosome was done for AZF region and SRY for cases and controls. RESULTS: We scored 16.1% of cases (19 out of 118) that bear one or more microdeletions in the studied loci and none among the controls. The aberrations were more frequent among azoospermic males (17 of 19) than in severe oligozoospermic subjects (2 of 19). CONCLUSION: Our study provides the results of screening of the largest Bengali infertile men sample genotyped with the maximum number of STS markers spanning the entire length of Y chromosome long arm. Y chromosome microdeletion is a significant genetic etiology of infertility among Bengali men. John Wiley and Sons Inc. 2021-08-24 /pmc/articles/PMC8580071/ /pubmed/34427986 http://dx.doi.org/10.1002/mgg3.1769 Text en © 2021 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Dutta, Saurav
Paladhi, Pranab
Pal, Samudra
Bose, Gunja
Ghosh, Papiya
Chattopadhyay, Ratna
Chakravarty, Baidyanath
Ghosh, Sujay
Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title_full Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title_fullStr Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title_full_unstemmed Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title_short Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India
title_sort prevalence of y chromosome microdeletion in azoospermia factor subregions among infertile men from west bengal, india
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580071/
https://www.ncbi.nlm.nih.gov/pubmed/34427986
http://dx.doi.org/10.1002/mgg3.1769
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