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Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis

Background: Approximately 50% of thymoma patients also show myasthenia gravis (MG), which is an autoimmune disease; however, the pathogenesis of MG-associated thymoma remains elusive. Our aim was to investigate immune-related lncRNA profiles of a set of candidate genes for better understanding of th...

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Autores principales: Zhuang, Jinman, Guan, Maohao, Liu, Maolin, Liu, Yuhang, Yang, Shuyan, Hu, Zhijian, Lai, Fancai, He, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580862/
https://www.ncbi.nlm.nih.gov/pubmed/34777476
http://dx.doi.org/10.3389/fgene.2021.756493
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author Zhuang, Jinman
Guan, Maohao
Liu, Maolin
Liu, Yuhang
Yang, Shuyan
Hu, Zhijian
Lai, Fancai
He, Fei
author_facet Zhuang, Jinman
Guan, Maohao
Liu, Maolin
Liu, Yuhang
Yang, Shuyan
Hu, Zhijian
Lai, Fancai
He, Fei
author_sort Zhuang, Jinman
collection PubMed
description Background: Approximately 50% of thymoma patients also show myasthenia gravis (MG), which is an autoimmune disease; however, the pathogenesis of MG-associated thymoma remains elusive. Our aim was to investigate immune-related lncRNA profiles of a set of candidate genes for better understanding of the molecular mechanism underlying the pathogenesis of thymoma with or without MG. Methods: Molecular profiles of thymoma with or without MG were downloaded from The Cancer Genome Atlas, and Pearson’s correlation analysis was performed to identify immune-related lncRNAs. T test was used to examine the differential expression and differential methylation between thymoma patients with or without MG. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to predict the function of target genes of immune-related lncRNAs. Results: Analyses of the 87 thymoma samples with complete MG information revealed that 205 mRNAs and 56 lncRNAs showed up-regulated expression in thymoma with MG patients, while 458 mRNAs and 84 lncRNAs showed down-regulated expression. The methylation level of three immune-related lncRNAs (AP000787.1, AC004943.1, WT1-AS, FOXG1-AS1) was significantly decreased in thymoma tissues, and the methylation level of these immune-related lncRNAs (WT1-AS: Cor = 0.368, p < 0.001; FOXG1-AS1: Cor = 0.288, p < 0.01; AC004943.1: Cor = -0.236, p < 0.05) correlated with their expression. GO and KEGG pathway analysis revealed that targets of the immune-related lncRNA FOXG1-AS1 were enriched in small GTPase binding and herpes simplex virus 1 infection. Transcription coregulator activity and cell cycle were the most enriched pathways for targets of lncRNA AC004943.1. LncRNA WT1-AS targets were most enriched in actin binding and axon guidance. Conclusion: Our results revealed the immune-related molecular profiling of thymoma with MG and without MG and identified key pathways involved in the underlying molecular mechanism of thymoma-related MG. These findings provide insights for further research of potential markers for thymoma-related MG.
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spelling pubmed-85808622021-11-12 Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis Zhuang, Jinman Guan, Maohao Liu, Maolin Liu, Yuhang Yang, Shuyan Hu, Zhijian Lai, Fancai He, Fei Front Genet Genetics Background: Approximately 50% of thymoma patients also show myasthenia gravis (MG), which is an autoimmune disease; however, the pathogenesis of MG-associated thymoma remains elusive. Our aim was to investigate immune-related lncRNA profiles of a set of candidate genes for better understanding of the molecular mechanism underlying the pathogenesis of thymoma with or without MG. Methods: Molecular profiles of thymoma with or without MG were downloaded from The Cancer Genome Atlas, and Pearson’s correlation analysis was performed to identify immune-related lncRNAs. T test was used to examine the differential expression and differential methylation between thymoma patients with or without MG. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to predict the function of target genes of immune-related lncRNAs. Results: Analyses of the 87 thymoma samples with complete MG information revealed that 205 mRNAs and 56 lncRNAs showed up-regulated expression in thymoma with MG patients, while 458 mRNAs and 84 lncRNAs showed down-regulated expression. The methylation level of three immune-related lncRNAs (AP000787.1, AC004943.1, WT1-AS, FOXG1-AS1) was significantly decreased in thymoma tissues, and the methylation level of these immune-related lncRNAs (WT1-AS: Cor = 0.368, p < 0.001; FOXG1-AS1: Cor = 0.288, p < 0.01; AC004943.1: Cor = -0.236, p < 0.05) correlated with their expression. GO and KEGG pathway analysis revealed that targets of the immune-related lncRNA FOXG1-AS1 were enriched in small GTPase binding and herpes simplex virus 1 infection. Transcription coregulator activity and cell cycle were the most enriched pathways for targets of lncRNA AC004943.1. LncRNA WT1-AS targets were most enriched in actin binding and axon guidance. Conclusion: Our results revealed the immune-related molecular profiling of thymoma with MG and without MG and identified key pathways involved in the underlying molecular mechanism of thymoma-related MG. These findings provide insights for further research of potential markers for thymoma-related MG. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8580862/ /pubmed/34777476 http://dx.doi.org/10.3389/fgene.2021.756493 Text en Copyright © 2021 Zhuang, Guan, Liu, Liu, Yang, Hu, Lai and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhuang, Jinman
Guan, Maohao
Liu, Maolin
Liu, Yuhang
Yang, Shuyan
Hu, Zhijian
Lai, Fancai
He, Fei
Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title_full Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title_fullStr Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title_full_unstemmed Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title_short Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis
title_sort immune-related molecular profiling of thymoma with myasthenia gravis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580862/
https://www.ncbi.nlm.nih.gov/pubmed/34777476
http://dx.doi.org/10.3389/fgene.2021.756493
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