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Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio

Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that in situ imbalance of T helper 17 cells (Th17...

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Autores principales: Mansour, Adel Abo, Raucci, Federica, Saviano, Anella, Tull, Samantha, Maione, Francesco, Iqbal, Asif Jilani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581207/
https://www.ncbi.nlm.nih.gov/pubmed/34777378
http://dx.doi.org/10.3389/fimmu.2021.762016
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author Mansour, Adel Abo
Raucci, Federica
Saviano, Anella
Tull, Samantha
Maione, Francesco
Iqbal, Asif Jilani
author_facet Mansour, Adel Abo
Raucci, Federica
Saviano, Anella
Tull, Samantha
Maione, Francesco
Iqbal, Asif Jilani
author_sort Mansour, Adel Abo
collection PubMed
description Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that in situ imbalance of T helper 17 cells (Th17)/regulatory T cells (Treg) impacts the subsequent damage to target tissues. Galectin-9 (Gal-9) is a modulator of innate and adaptive immunity with both pro- and anti-inflammatory functions, dependent upon its expression and cellular location. However, the specific cellular and molecular mechanisms by which Gal-9 modulates the inflammatory response in the onset and progression of gouty arthritis has yet to be elucidated. In this study, we sought to comprehensively characterise the functional role of exogenous Gal-9 in an in vivo model of MSU crystal-induced gouty inflammation by monitoring in situ neutrophils, monocytes and Th17/Treg recruited phenotypes and related cyto-chemokines profile. Treatment with Gal-9 revealed a dose-dependent reduction in joint inflammation scores, knee joint oedema and expression of different pro-inflammatory cyto-chemokines. Furthermore, flow cytometry analysis highlighted a significant modulation of infiltrating inflammatory monocytes (CD11b(+)/CD115(+)/LY6-C(hi)) and Th17 (CD4(+)/IL-17(+))/Treg (CD4(+)/CD25(+)/FOXP-3(+)) cells following Gal-9 treatment. Collectively the results presented in this study indicate that the administration of Gal-9 could provide a new therapeutic strategy for preventing tissue damage in gouty arthritic inflammation and, possibly, in other inflammatory-based diseases.
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spelling pubmed-85812072021-11-12 Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio Mansour, Adel Abo Raucci, Federica Saviano, Anella Tull, Samantha Maione, Francesco Iqbal, Asif Jilani Front Immunol Immunology Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that in situ imbalance of T helper 17 cells (Th17)/regulatory T cells (Treg) impacts the subsequent damage to target tissues. Galectin-9 (Gal-9) is a modulator of innate and adaptive immunity with both pro- and anti-inflammatory functions, dependent upon its expression and cellular location. However, the specific cellular and molecular mechanisms by which Gal-9 modulates the inflammatory response in the onset and progression of gouty arthritis has yet to be elucidated. In this study, we sought to comprehensively characterise the functional role of exogenous Gal-9 in an in vivo model of MSU crystal-induced gouty inflammation by monitoring in situ neutrophils, monocytes and Th17/Treg recruited phenotypes and related cyto-chemokines profile. Treatment with Gal-9 revealed a dose-dependent reduction in joint inflammation scores, knee joint oedema and expression of different pro-inflammatory cyto-chemokines. Furthermore, flow cytometry analysis highlighted a significant modulation of infiltrating inflammatory monocytes (CD11b(+)/CD115(+)/LY6-C(hi)) and Th17 (CD4(+)/IL-17(+))/Treg (CD4(+)/CD25(+)/FOXP-3(+)) cells following Gal-9 treatment. Collectively the results presented in this study indicate that the administration of Gal-9 could provide a new therapeutic strategy for preventing tissue damage in gouty arthritic inflammation and, possibly, in other inflammatory-based diseases. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8581207/ /pubmed/34777378 http://dx.doi.org/10.3389/fimmu.2021.762016 Text en Copyright © 2021 Mansour, Raucci, Saviano, Tull, Maione and Iqbal https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mansour, Adel Abo
Raucci, Federica
Saviano, Anella
Tull, Samantha
Maione, Francesco
Iqbal, Asif Jilani
Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title_full Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title_fullStr Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title_full_unstemmed Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title_short Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio
title_sort galectin-9 regulates monosodium urate crystal-induced gouty inflammation through the modulation of treg/th17 ratio
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581207/
https://www.ncbi.nlm.nih.gov/pubmed/34777378
http://dx.doi.org/10.3389/fimmu.2021.762016
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