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Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury

Hypoxic‐ischaemic encephalopathy (HIE) is a type of brain injury affecting approximately 1 million newborn babies per year worldwide, the only treatment for which is therapeutic hypothermia. Thrombin‐preconditioned mesenchymal stem cells (MSCs) exert neuroprotective effects by enriching cargo conten...

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Autores principales: Noh, Jung‐Ho, Jeong, Ji‐Seong, Park, Sang‐Jin, Jin Jung, Kyung, Lee, Byoung‐Seok, Kim, Woo‐Jin, Han, Ji‐Seok, Cho, Min‐Kyung, Sung, Dong Kyung, Ahn, So Yoon, Chang, Yun Sil, Son, Hwa‐Young, Jeong, Eun Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581315/
https://www.ncbi.nlm.nih.gov/pubmed/34651412
http://dx.doi.org/10.1111/jcmm.16971
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author Noh, Jung‐Ho
Jeong, Ji‐Seong
Park, Sang‐Jin
Jin Jung, Kyung
Lee, Byoung‐Seok
Kim, Woo‐Jin
Han, Ji‐Seok
Cho, Min‐Kyung
Sung, Dong Kyung
Ahn, So Yoon
Chang, Yun Sil
Son, Hwa‐Young
Jeong, Eun Ju
author_facet Noh, Jung‐Ho
Jeong, Ji‐Seong
Park, Sang‐Jin
Jin Jung, Kyung
Lee, Byoung‐Seok
Kim, Woo‐Jin
Han, Ji‐Seok
Cho, Min‐Kyung
Sung, Dong Kyung
Ahn, So Yoon
Chang, Yun Sil
Son, Hwa‐Young
Jeong, Eun Ju
author_sort Noh, Jung‐Ho
collection PubMed
description Hypoxic‐ischaemic encephalopathy (HIE) is a type of brain injury affecting approximately 1 million newborn babies per year worldwide, the only treatment for which is therapeutic hypothermia. Thrombin‐preconditioned mesenchymal stem cells (MSCs) exert neuroprotective effects by enriching cargo contents and boosting exosome biogenesis, thus showing promise as a new therapeutic strategy for HIE. This study was conducted to evaluate the tissue distribution and potential toxicity of thrombin‐preconditioned human Wharton's jelly‐derived mesenchymal stem cells (th‐hWJMSCs) in animal models before the initiation of clinical trials. We investigated the biodistribution, tumorigenicity and general toxicity of th‐hWJMSCs. MSCs were administered the maximum feasible dose (1 × 10(5) cells/10 µL/head) once, or at lower doses into the cerebral ventricle. To support the clinical use of th‐hWJMSCs for treating brain injury, preclinical safety studies were conducted in newborn Sprague‐Dawley rats and BALB/c nude mice. In addition, growth parameters were evaluated to assess the impact of th‐hWJMSCs on the growth of newborn babies. Our results suggest that th‐hWJMSCs are non‐toxic and non‐tumorigenic in rodent models, survive for up to 7 days in the brain and hold potential for HIE therapy.
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spelling pubmed-85813152021-11-17 Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury Noh, Jung‐Ho Jeong, Ji‐Seong Park, Sang‐Jin Jin Jung, Kyung Lee, Byoung‐Seok Kim, Woo‐Jin Han, Ji‐Seok Cho, Min‐Kyung Sung, Dong Kyung Ahn, So Yoon Chang, Yun Sil Son, Hwa‐Young Jeong, Eun Ju J Cell Mol Med Original Articles Hypoxic‐ischaemic encephalopathy (HIE) is a type of brain injury affecting approximately 1 million newborn babies per year worldwide, the only treatment for which is therapeutic hypothermia. Thrombin‐preconditioned mesenchymal stem cells (MSCs) exert neuroprotective effects by enriching cargo contents and boosting exosome biogenesis, thus showing promise as a new therapeutic strategy for HIE. This study was conducted to evaluate the tissue distribution and potential toxicity of thrombin‐preconditioned human Wharton's jelly‐derived mesenchymal stem cells (th‐hWJMSCs) in animal models before the initiation of clinical trials. We investigated the biodistribution, tumorigenicity and general toxicity of th‐hWJMSCs. MSCs were administered the maximum feasible dose (1 × 10(5) cells/10 µL/head) once, or at lower doses into the cerebral ventricle. To support the clinical use of th‐hWJMSCs for treating brain injury, preclinical safety studies were conducted in newborn Sprague‐Dawley rats and BALB/c nude mice. In addition, growth parameters were evaluated to assess the impact of th‐hWJMSCs on the growth of newborn babies. Our results suggest that th‐hWJMSCs are non‐toxic and non‐tumorigenic in rodent models, survive for up to 7 days in the brain and hold potential for HIE therapy. John Wiley and Sons Inc. 2021-10-15 2021-11 /pmc/articles/PMC8581315/ /pubmed/34651412 http://dx.doi.org/10.1111/jcmm.16971 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Noh, Jung‐Ho
Jeong, Ji‐Seong
Park, Sang‐Jin
Jin Jung, Kyung
Lee, Byoung‐Seok
Kim, Woo‐Jin
Han, Ji‐Seok
Cho, Min‐Kyung
Sung, Dong Kyung
Ahn, So Yoon
Chang, Yun Sil
Son, Hwa‐Young
Jeong, Eun Ju
Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title_full Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title_fullStr Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title_full_unstemmed Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title_short Preclinical assessment of thrombin‐preconditioned human Wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
title_sort preclinical assessment of thrombin‐preconditioned human wharton’s jelly‐derived mesenchymal stem cells for neonatal hypoxic‐ischaemic brain injury
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581315/
https://www.ncbi.nlm.nih.gov/pubmed/34651412
http://dx.doi.org/10.1111/jcmm.16971
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