Cargando…

Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea

Dysmenorrhea refers to a kind of uterine cramping pain that occurs in women during the period of menstrual. Guizhi Fuling Capsules are mainly used for the treatment of various pain syndromes and especially effective in treating primary dysmenorrhea. However, the research on its modern pharmacology a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yu, Su, Na, Liu, Weiyi, Wang, Qingqing, Sun, Jianguo, Peng, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581447/
https://www.ncbi.nlm.nih.gov/pubmed/34776980
http://dx.doi.org/10.3389/fphar.2021.764904
_version_ 1784596810850893824
author Zhang, Yu
Su, Na
Liu, Weiyi
Wang, Qingqing
Sun, Jianguo
Peng, Ying
author_facet Zhang, Yu
Su, Na
Liu, Weiyi
Wang, Qingqing
Sun, Jianguo
Peng, Ying
author_sort Zhang, Yu
collection PubMed
description Dysmenorrhea refers to a kind of uterine cramping pain that occurs in women during the period of menstrual. Guizhi Fuling Capsules are mainly used for the treatment of various pain syndromes and especially effective in treating primary dysmenorrhea. However, the research on its modern pharmacology and mechanism of action have not been thoroughly carried out. It is not clear about the main active ingredients, potential targets and metabolic pathways involved in its efficacy. Therefore, this research project employed estradiol benzoate sensitization combined with oxytocin pain to construct the cold coagulation syndrome dysmenorrhea model, observed the anti-dysmenorrhea effect of Guizhi Fuling Capsules, and used the metabolomics to explore its mechanism. The results showed that Guizhi Fuling Capsules could considerably reduce the number of writhing times in dysmenorrhea rats, increasing the level of PGE2 and β-EP and reducing the contents of PGF2α in rat serum. Pathological sections of uterus and ovaries also showed that Guizhi Fuling Capsules could significantly relieve endometrial hyperplasia and improve ovarian function. The LC/MS-based metabolomics of rat uterine indicated that the model group has a great deviation from the control group. Compared with the model group, the Guizhi Fuling Capsules group had a tendency to shift to the control group, and the main metabolic changes was mainly concentrated on saturated and unsaturated fatty acids. Among them, arachidonic acid is in a pivotal position, and the expression of its rate-limiting enzyme (COX-2) involved in its cyclooxygenase metabolic pathway was significantly up-regulated in the model group, but significantly decreased after the intervention of Guizhi Fuling Capsules. In conclusion, Guizhi Fuling Capsules can effectively relieve primary dysmenorrhea, and this effect may be attributed to the regulation effects of Guizhi Fuling Capsules on endogenous metabolism, such as inhibiting arachidonic acid converted to prostaglandins through downregulate the expression of COX-2, which plays an anti-inflammatory effect.
format Online
Article
Text
id pubmed-8581447
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85814472021-11-12 Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea Zhang, Yu Su, Na Liu, Weiyi Wang, Qingqing Sun, Jianguo Peng, Ying Front Pharmacol Pharmacology Dysmenorrhea refers to a kind of uterine cramping pain that occurs in women during the period of menstrual. Guizhi Fuling Capsules are mainly used for the treatment of various pain syndromes and especially effective in treating primary dysmenorrhea. However, the research on its modern pharmacology and mechanism of action have not been thoroughly carried out. It is not clear about the main active ingredients, potential targets and metabolic pathways involved in its efficacy. Therefore, this research project employed estradiol benzoate sensitization combined with oxytocin pain to construct the cold coagulation syndrome dysmenorrhea model, observed the anti-dysmenorrhea effect of Guizhi Fuling Capsules, and used the metabolomics to explore its mechanism. The results showed that Guizhi Fuling Capsules could considerably reduce the number of writhing times in dysmenorrhea rats, increasing the level of PGE2 and β-EP and reducing the contents of PGF2α in rat serum. Pathological sections of uterus and ovaries also showed that Guizhi Fuling Capsules could significantly relieve endometrial hyperplasia and improve ovarian function. The LC/MS-based metabolomics of rat uterine indicated that the model group has a great deviation from the control group. Compared with the model group, the Guizhi Fuling Capsules group had a tendency to shift to the control group, and the main metabolic changes was mainly concentrated on saturated and unsaturated fatty acids. Among them, arachidonic acid is in a pivotal position, and the expression of its rate-limiting enzyme (COX-2) involved in its cyclooxygenase metabolic pathway was significantly up-regulated in the model group, but significantly decreased after the intervention of Guizhi Fuling Capsules. In conclusion, Guizhi Fuling Capsules can effectively relieve primary dysmenorrhea, and this effect may be attributed to the regulation effects of Guizhi Fuling Capsules on endogenous metabolism, such as inhibiting arachidonic acid converted to prostaglandins through downregulate the expression of COX-2, which plays an anti-inflammatory effect. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8581447/ /pubmed/34776980 http://dx.doi.org/10.3389/fphar.2021.764904 Text en Copyright © 2021 Zhang, Su, Liu, Wang, Sun and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Yu
Su, Na
Liu, Weiyi
Wang, Qingqing
Sun, Jianguo
Peng, Ying
Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title_full Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title_fullStr Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title_full_unstemmed Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title_short Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea
title_sort metabolomics study of guizhi fuling capsules in rats with cold coagulation dysmenorrhea
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581447/
https://www.ncbi.nlm.nih.gov/pubmed/34776980
http://dx.doi.org/10.3389/fphar.2021.764904
work_keys_str_mv AT zhangyu metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea
AT suna metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea
AT liuweiyi metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea
AT wangqingqing metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea
AT sunjianguo metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea
AT pengying metabolomicsstudyofguizhifulingcapsulesinratswithcoldcoagulationdysmenorrhea