Cargando…

Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae

Thread blight disease has recently been described as an emerging disease on cacao (Theobroma cacao) in Ghana. In Ghana, thread blight disease is caused by multiple species of the Marasmiaceae family: Marasmius tenuissimus, M. crinis-equi, M. palmivorus, and Marasmiellus scandens. Interestingly, two...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali, Shahin S., Amoako-Attah, Ishmael, Shao, Jonathan, Kumi-Asare, Eric, Meinhardt, Lyndel W., Bailey, Bryan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581569/
https://www.ncbi.nlm.nih.gov/pubmed/34777305
http://dx.doi.org/10.3389/fmicb.2021.752094
_version_ 1784596834879012864
author Ali, Shahin S.
Amoako-Attah, Ishmael
Shao, Jonathan
Kumi-Asare, Eric
Meinhardt, Lyndel W.
Bailey, Bryan A.
author_facet Ali, Shahin S.
Amoako-Attah, Ishmael
Shao, Jonathan
Kumi-Asare, Eric
Meinhardt, Lyndel W.
Bailey, Bryan A.
author_sort Ali, Shahin S.
collection PubMed
description Thread blight disease has recently been described as an emerging disease on cacao (Theobroma cacao) in Ghana. In Ghana, thread blight disease is caused by multiple species of the Marasmiaceae family: Marasmius tenuissimus, M. crinis-equi, M. palmivorus, and Marasmiellus scandens. Interestingly, two additional members of the Marasmiaceae; Moniliophthora roreri (frosty pod rot) and Moniliophthora perniciosa (witches’ broom disease), are major pathogens of cacao in the Western hemisphere. It is important to accurately characterize the genetic relationships among these economically important species in support of their disease management. We used data from Illumina NGS-based genome sequencing efforts to study the mitochondrial genomes (mitogenomes) of the four cacao thread blight associated pathogens from Ghana and compared them with published mitogenomes of Mon. roreri and Mon. perniciosa. There is a remarkable interspecies variation in mitogenome size within the six cacao-associated Marasmiaceae species, ranging from 43,121 to 109,103 bp. The differences in genome lengths are primarily due to the number and lengths of introns, differences in intergenic space, and differences in the size and numbers of unidentified ORFs (uORF). Among seven M. tenuissimus mitogenomes sequenced, there is variation in size and sequence pointing to divergent evolution patterns within the species. The intronic regions show a high degree of sequence variation compared to the conserved sequences of the 14 core genes. The intronic ORFs identified, regardless of species, encode GIY-YIG or LAGLIDADG domain-containing homing endonuclease genes. Phylogenetic relationships using the 14 core proteins largely mimic the phylogenetic relationships observed in gene order patterns, grouping M. tenuissimus with M. crinis-equi, and M. palmivorus with Mon. roreri and Mon. perniciosa, leaving Mar. scandens as an outlier. The results from this study provide evidence of independent expansion/contraction events and sequence diversification in each species and establish a foundation for further exploration of the evolutionary trajectory of the fungi in Marasmiaceae family.
format Online
Article
Text
id pubmed-8581569
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85815692021-11-12 Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae Ali, Shahin S. Amoako-Attah, Ishmael Shao, Jonathan Kumi-Asare, Eric Meinhardt, Lyndel W. Bailey, Bryan A. Front Microbiol Microbiology Thread blight disease has recently been described as an emerging disease on cacao (Theobroma cacao) in Ghana. In Ghana, thread blight disease is caused by multiple species of the Marasmiaceae family: Marasmius tenuissimus, M. crinis-equi, M. palmivorus, and Marasmiellus scandens. Interestingly, two additional members of the Marasmiaceae; Moniliophthora roreri (frosty pod rot) and Moniliophthora perniciosa (witches’ broom disease), are major pathogens of cacao in the Western hemisphere. It is important to accurately characterize the genetic relationships among these economically important species in support of their disease management. We used data from Illumina NGS-based genome sequencing efforts to study the mitochondrial genomes (mitogenomes) of the four cacao thread blight associated pathogens from Ghana and compared them with published mitogenomes of Mon. roreri and Mon. perniciosa. There is a remarkable interspecies variation in mitogenome size within the six cacao-associated Marasmiaceae species, ranging from 43,121 to 109,103 bp. The differences in genome lengths are primarily due to the number and lengths of introns, differences in intergenic space, and differences in the size and numbers of unidentified ORFs (uORF). Among seven M. tenuissimus mitogenomes sequenced, there is variation in size and sequence pointing to divergent evolution patterns within the species. The intronic regions show a high degree of sequence variation compared to the conserved sequences of the 14 core genes. The intronic ORFs identified, regardless of species, encode GIY-YIG or LAGLIDADG domain-containing homing endonuclease genes. Phylogenetic relationships using the 14 core proteins largely mimic the phylogenetic relationships observed in gene order patterns, grouping M. tenuissimus with M. crinis-equi, and M. palmivorus with Mon. roreri and Mon. perniciosa, leaving Mar. scandens as an outlier. The results from this study provide evidence of independent expansion/contraction events and sequence diversification in each species and establish a foundation for further exploration of the evolutionary trajectory of the fungi in Marasmiaceae family. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8581569/ /pubmed/34777305 http://dx.doi.org/10.3389/fmicb.2021.752094 Text en Copyright © 2021 Ali, Amoako-Attah, Shao, Kumi-Asare, Meinhardt and Bailey. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ali, Shahin S.
Amoako-Attah, Ishmael
Shao, Jonathan
Kumi-Asare, Eric
Meinhardt, Lyndel W.
Bailey, Bryan A.
Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title_full Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title_fullStr Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title_full_unstemmed Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title_short Mitochondrial Genomics of Six Cacao Pathogens From the Basidiomycete Family Marasmiaceae
title_sort mitochondrial genomics of six cacao pathogens from the basidiomycete family marasmiaceae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581569/
https://www.ncbi.nlm.nih.gov/pubmed/34777305
http://dx.doi.org/10.3389/fmicb.2021.752094
work_keys_str_mv AT alishahins mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae
AT amoakoattahishmael mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae
AT shaojonathan mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae
AT kumiasareeric mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae
AT meinhardtlyndelw mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae
AT baileybryana mitochondrialgenomicsofsixcacaopathogensfromthebasidiomycetefamilymarasmiaceae