Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the pr...

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Detalles Bibliográficos
Autores principales: Guo, Xinzheng, Starr, Christopher, Zhou, Jing, Chen, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581646/
https://www.ncbi.nlm.nih.gov/pubmed/34806045
http://dx.doi.org/10.1016/j.xpro.2021.100932
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author Guo, Xinzheng
Starr, Christopher
Zhou, Jing
Chen, Bo
author_facet Guo, Xinzheng
Starr, Christopher
Zhou, Jing
Chen, Bo
author_sort Guo, Xinzheng
collection PubMed
description The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).
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spelling pubmed-85816462021-11-18 Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells Guo, Xinzheng Starr, Christopher Zhou, Jing Chen, Bo STAR Protoc Protocol The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021). Elsevier 2021-11-03 /pmc/articles/PMC8581646/ /pubmed/34806045 http://dx.doi.org/10.1016/j.xpro.2021.100932 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Guo, Xinzheng
Starr, Christopher
Zhou, Jing
Chen, Bo
Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_full Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_fullStr Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_full_unstemmed Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_short Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_sort protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581646/
https://www.ncbi.nlm.nih.gov/pubmed/34806045
http://dx.doi.org/10.1016/j.xpro.2021.100932
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