Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation detection in single cells. We identify thousands...

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Detalles Bibliográficos
Autores principales: Tian, Luyi, Jabbari, Jafar S., Thijssen, Rachel, Gouil, Quentin, Amarasinghe, Shanika L., Voogd, Oliver, Kariyawasam, Hasaru, Du, Mei R. M., Schuster, Jakob, Wang, Changqing, Su, Shian, Dong, Xueyi, Law, Charity W., Lucattini, Alexis, Prawer, Yair David Joseph, Collar-Fernández, Coralina, Chung, Jin D., Naim, Timur, Chan, Audrey, Ly, Chi Hai, Lynch, Gordon S., Ryall, James G., Anttila, Casey J. A., Peng, Hongke, Anderson, Mary Ann, Flensburg, Christoffer, Majewski, Ian, Roberts, Andrew W., Huang, David C. S., Clark, Michael B., Ritchie, Matthew E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582192/
https://www.ncbi.nlm.nih.gov/pubmed/34763716
http://dx.doi.org/10.1186/s13059-021-02525-6

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