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ZjSEP3 modulates flowering time by regulating the LHY promoter
BACKGROUND: SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. RESULTS: Here, we investigated that SEP3 i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582215/ https://www.ncbi.nlm.nih.gov/pubmed/34763664 http://dx.doi.org/10.1186/s12870-021-03305-x |
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author | Gao, Weilin Zhang, Liman Wang, Jiurui Liu, Zhiguo Zhang, Yao Xue, Chaoling Liu, Mengjun Zhao, Jin |
author_facet | Gao, Weilin Zhang, Liman Wang, Jiurui Liu, Zhiguo Zhang, Yao Xue, Chaoling Liu, Mengjun Zhao, Jin |
author_sort | Gao, Weilin |
collection | PubMed |
description | BACKGROUND: SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. RESULTS: Here, we investigated that SEP3 in Ziziphus jujuba Mill. (ZjSEP3) was expressed in four floral organs and exhibited strong transcriptional activation activity. ZjSEP3 transgenic Arabidopsis showed an early-flowering phenotype and altered the expression of some genes related to flowering. Among them, the expression of LATE ELONGATED HYPOCOTYL (AtLHY), the key gene of circadian rhythms, was significantly suppressed. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assays (EMSAs) further verified that ZjSEP3 inhibited the transcription of AtLHY by binding to the CArG-boxes in its promoter. Moreover, ZjSEP3 also could bind to the ZjLHY promoter and the conserved binding regions of ZjSEP3 were found in the LHY promoter of various plant species. The ectopic regulatory pathway of ZjSEP3-AtLHY was further supported by the ability of 35S::AtLHY to rescue the early-flowering phenotype in ZjSEP3 transgenic plants. In ZjSEP3 transgenic plants, total chlorophyll content and the expression of genes involved in chlorophyll synthesis increased during vegetative stages, which should contribute to its early flowering and relate to the regulatory of AtLHY. CONCLUSION: Overall, ZjSEP3-AtLHY pathway represents a novel regulatory mechanism that is involved in the regulation of flowering time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03305-x. |
format | Online Article Text |
id | pubmed-8582215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-85822152021-11-15 ZjSEP3 modulates flowering time by regulating the LHY promoter Gao, Weilin Zhang, Liman Wang, Jiurui Liu, Zhiguo Zhang, Yao Xue, Chaoling Liu, Mengjun Zhao, Jin BMC Plant Biol Research BACKGROUND: SEPALLATA3 (SEP3), which is conserved across various plant species, plays essential and various roles in flower and fruit development. However, the regulatory network of the role of SEP3 in flowering time at the molecular level remained unclear. RESULTS: Here, we investigated that SEP3 in Ziziphus jujuba Mill. (ZjSEP3) was expressed in four floral organs and exhibited strong transcriptional activation activity. ZjSEP3 transgenic Arabidopsis showed an early-flowering phenotype and altered the expression of some genes related to flowering. Among them, the expression of LATE ELONGATED HYPOCOTYL (AtLHY), the key gene of circadian rhythms, was significantly suppressed. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assays (EMSAs) further verified that ZjSEP3 inhibited the transcription of AtLHY by binding to the CArG-boxes in its promoter. Moreover, ZjSEP3 also could bind to the ZjLHY promoter and the conserved binding regions of ZjSEP3 were found in the LHY promoter of various plant species. The ectopic regulatory pathway of ZjSEP3-AtLHY was further supported by the ability of 35S::AtLHY to rescue the early-flowering phenotype in ZjSEP3 transgenic plants. In ZjSEP3 transgenic plants, total chlorophyll content and the expression of genes involved in chlorophyll synthesis increased during vegetative stages, which should contribute to its early flowering and relate to the regulatory of AtLHY. CONCLUSION: Overall, ZjSEP3-AtLHY pathway represents a novel regulatory mechanism that is involved in the regulation of flowering time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03305-x. BioMed Central 2021-11-11 /pmc/articles/PMC8582215/ /pubmed/34763664 http://dx.doi.org/10.1186/s12870-021-03305-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Gao, Weilin Zhang, Liman Wang, Jiurui Liu, Zhiguo Zhang, Yao Xue, Chaoling Liu, Mengjun Zhao, Jin ZjSEP3 modulates flowering time by regulating the LHY promoter |
title | ZjSEP3 modulates flowering time by regulating the LHY promoter |
title_full | ZjSEP3 modulates flowering time by regulating the LHY promoter |
title_fullStr | ZjSEP3 modulates flowering time by regulating the LHY promoter |
title_full_unstemmed | ZjSEP3 modulates flowering time by regulating the LHY promoter |
title_short | ZjSEP3 modulates flowering time by regulating the LHY promoter |
title_sort | zjsep3 modulates flowering time by regulating the lhy promoter |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582215/ https://www.ncbi.nlm.nih.gov/pubmed/34763664 http://dx.doi.org/10.1186/s12870-021-03305-x |
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