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Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales

Fruit house microbial communities that are unique from the rest of the plant. While symbiotic microbial communities complete important functions for their hosts, the fruit microbiome is often understudied compared to other plant organs. Fruits are reproductive tissues that house, protect, and facili...

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Autores principales: Heminger, Ariel R., Belden, Lisa K., Barney, Jacob N., Badgley, Brian D., Haak, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582302/
https://www.ncbi.nlm.nih.gov/pubmed/34820171
http://dx.doi.org/10.7717/peerj.12359
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author Heminger, Ariel R.
Belden, Lisa K.
Barney, Jacob N.
Badgley, Brian D.
Haak, David C.
author_facet Heminger, Ariel R.
Belden, Lisa K.
Barney, Jacob N.
Badgley, Brian D.
Haak, David C.
author_sort Heminger, Ariel R.
collection PubMed
description Fruit house microbial communities that are unique from the rest of the plant. While symbiotic microbial communities complete important functions for their hosts, the fruit microbiome is often understudied compared to other plant organs. Fruits are reproductive tissues that house, protect, and facilitate the dispersal of seeds, and thus they are directly tied to plant fitness. Fruit microbial communities may, therefore, also impact plant fitness. In this study, we assessed how bacterial communities associated with fruit of Solanum carolinense, a native herbaceous perennial weed, vary at fine spatial scales (<0.5 km). A majority of the studies conducted on plant microbial communities have been done at large spatial scales and have observed microbial community variation across these large spatial scales. However, both the environment and pollinators play a role in shaping plant microbial communities and likely have impacts on the plant microbiome at fine scales. We collected fruit samples from eight sampling locations, ranging from 2 to 450 m apart, and assessed the fruit bacterial communities using 16S rRNA gene amplicon sequencing. Overall, we found no differences in observed richness or microbial community composition among sampling locations. Bacterial community structure of fruits collected near one another were not more different than those that were farther apart at the scales we examined. These fine spatial scales are important to obligate out-crossing plant species such as S. carolinense because they are ecologically relevant to pollinators. Thus, our results could imply that pollinators serve to homogenize fruit bacterial communities across these smaller scales.
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spelling pubmed-85823022021-11-23 Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales Heminger, Ariel R. Belden, Lisa K. Barney, Jacob N. Badgley, Brian D. Haak, David C. PeerJ Bioinformatics Fruit house microbial communities that are unique from the rest of the plant. While symbiotic microbial communities complete important functions for their hosts, the fruit microbiome is often understudied compared to other plant organs. Fruits are reproductive tissues that house, protect, and facilitate the dispersal of seeds, and thus they are directly tied to plant fitness. Fruit microbial communities may, therefore, also impact plant fitness. In this study, we assessed how bacterial communities associated with fruit of Solanum carolinense, a native herbaceous perennial weed, vary at fine spatial scales (<0.5 km). A majority of the studies conducted on plant microbial communities have been done at large spatial scales and have observed microbial community variation across these large spatial scales. However, both the environment and pollinators play a role in shaping plant microbial communities and likely have impacts on the plant microbiome at fine scales. We collected fruit samples from eight sampling locations, ranging from 2 to 450 m apart, and assessed the fruit bacterial communities using 16S rRNA gene amplicon sequencing. Overall, we found no differences in observed richness or microbial community composition among sampling locations. Bacterial community structure of fruits collected near one another were not more different than those that were farther apart at the scales we examined. These fine spatial scales are important to obligate out-crossing plant species such as S. carolinense because they are ecologically relevant to pollinators. Thus, our results could imply that pollinators serve to homogenize fruit bacterial communities across these smaller scales. PeerJ Inc. 2021-11-08 /pmc/articles/PMC8582302/ /pubmed/34820171 http://dx.doi.org/10.7717/peerj.12359 Text en ©2021 Heminger et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Heminger, Ariel R.
Belden, Lisa K.
Barney, Jacob N.
Badgley, Brian D.
Haak, David C.
Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title_full Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title_fullStr Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title_full_unstemmed Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title_short Horsenettle (Solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
title_sort horsenettle (solanum carolinense) fruit bacterial communities are not variable across fine spatial scales
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582302/
https://www.ncbi.nlm.nih.gov/pubmed/34820171
http://dx.doi.org/10.7717/peerj.12359
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