Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was s...

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Detalles Bibliográficos
Autores principales: Pessoa-e-Silva, Rômulo, de Oliveira, Priscilla Stela Santana, Gonçalves, Sayonara Maria Calado, Guarines, Klarissa Miranda, Carvalho, Lidiane Vasconcelos do Nascimento, Correia, Maria Andreza Bezerra, da Rosa, Michelle Melgarejo, Rêgo, Moacyr Jesus Barreto de Melo, Pitta, Maira Galdino da Rocha, Pereira, Michelly Cristiny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582969/
https://www.ncbi.nlm.nih.gov/pubmed/34787260
http://dx.doi.org/10.1590/0037-8682-0270-2021
Descripción
Sumario:INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS: Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS: The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

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