Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was s...

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Autores principales: Pessoa-e-Silva, Rômulo, de Oliveira, Priscilla Stela Santana, Gonçalves, Sayonara Maria Calado, Guarines, Klarissa Miranda, Carvalho, Lidiane Vasconcelos do Nascimento, Correia, Maria Andreza Bezerra, da Rosa, Michelle Melgarejo, Rêgo, Moacyr Jesus Barreto de Melo, Pitta, Maira Galdino da Rocha, Pereira, Michelly Cristiny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582969/
https://www.ncbi.nlm.nih.gov/pubmed/34787260
http://dx.doi.org/10.1590/0037-8682-0270-2021
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author Pessoa-e-Silva, Rômulo
de Oliveira, Priscilla Stela Santana
Gonçalves, Sayonara Maria Calado
Guarines, Klarissa Miranda
Carvalho, Lidiane Vasconcelos do Nascimento
Correia, Maria Andreza Bezerra
da Rosa, Michelle Melgarejo
Rêgo, Moacyr Jesus Barreto de Melo
Pitta, Maira Galdino da Rocha
Pereira, Michelly Cristiny
author_facet Pessoa-e-Silva, Rômulo
de Oliveira, Priscilla Stela Santana
Gonçalves, Sayonara Maria Calado
Guarines, Klarissa Miranda
Carvalho, Lidiane Vasconcelos do Nascimento
Correia, Maria Andreza Bezerra
da Rosa, Michelle Melgarejo
Rêgo, Moacyr Jesus Barreto de Melo
Pitta, Maira Galdino da Rocha
Pereira, Michelly Cristiny
author_sort Pessoa-e-Silva, Rômulo
collection PubMed
description INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS: Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS: The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.
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spelling pubmed-85829692021-11-18 Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols Pessoa-e-Silva, Rômulo de Oliveira, Priscilla Stela Santana Gonçalves, Sayonara Maria Calado Guarines, Klarissa Miranda Carvalho, Lidiane Vasconcelos do Nascimento Correia, Maria Andreza Bezerra da Rosa, Michelle Melgarejo Rêgo, Moacyr Jesus Barreto de Melo Pitta, Maira Galdino da Rocha Pereira, Michelly Cristiny Rev Soc Bras Med Trop Short Communication INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS: Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS: The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations. Sociedade Brasileira de Medicina Tropical - SBMT 2021-11-12 /pmc/articles/PMC8582969/ /pubmed/34787260 http://dx.doi.org/10.1590/0037-8682-0270-2021 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Pessoa-e-Silva, Rômulo
de Oliveira, Priscilla Stela Santana
Gonçalves, Sayonara Maria Calado
Guarines, Klarissa Miranda
Carvalho, Lidiane Vasconcelos do Nascimento
Correia, Maria Andreza Bezerra
da Rosa, Michelle Melgarejo
Rêgo, Moacyr Jesus Barreto de Melo
Pitta, Maira Galdino da Rocha
Pereira, Michelly Cristiny
Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_full Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_fullStr Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_full_unstemmed Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_short Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
title_sort enhanced rapid commercial dna extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: easy adaptation to current protocols
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582969/
https://www.ncbi.nlm.nih.gov/pubmed/34787260
http://dx.doi.org/10.1590/0037-8682-0270-2021
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